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Assessment of genetic diversity in Mucuna species of India using randomly amplified polymorphic DNA and inter simple sequence repeat markers

Abstract

Genus Mucuna which is native to China and Eastern India comprises of perennial climbing legume with long slender branches, trifoliate leaves and bear green or brown pod covered with soft or rigid hairs that cause intense irritation. The plants of this genus are agronomically and economically important and commercially cultivated in India, China and other regions of the world. The high degrees of taxonomical confusions exist in Mucuna species that make authentic identification and classification difficult. In the present study, the genetic diversity among the 59 accessions of six species and three varieties of M. pruriens has been assessed using DNA fingerprinting based molecular markers techniques namely randomly amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and combined dataset of RAPD and ISSR. Also, genetic relationship among two endemic species of Mucuna namely M. imbricata and M. macrocarpa and two varieties namely IIHR hybrid (MHR) and Dhanwantari (MD) with other species under study was investigated by using cluster analysis and principal coordinate analysis. The cluster analysis of RAPD, ISSR and combined dataset of RAPD and ISSR clearly demonstrated the existence of high interspecific variation than intra-specific variation in genus Mucuna. The utility and efficacy of RAPD and ISSR for the study of intra species and interspecies genetic diversity was evident from AMOVA and PCoA analysis. This study demonstrates the genetic diversity in Mucuna species and indicates that these markers could be successfully used to assess genetic variation among the accessions of Mucuna species.

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Acknowledgments

This study was funded by Department of Biotechnology, Govt. of India and was carried out as a part of SUK-DBT IPLS program ((Ref. No.: BT/PR4572/INF/22/147/2012.) sanctioned to Department of Biotechnology, Shivaji University, Kolhapur, India. Dr. Kiran D. Pawar gratefully acknowledges SUK-DBT IPLS program for funding his tenure as Assistant Professor. Mr. Ravishankar R. Patil and Ms. Manali R. Rane acknowledge SUK-DBT IPLS program for fellowship. Prof. V. A. Bapat is thankful to Indian National Science Academy, New Delhi, India for senior scientist fellowship.

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Correspondence to Jyoti P. Jadhav.

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Ravishankar R. Patil and Kiran D. Pawar contributed equally (shared first author).

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Patil, R.R., Pawar, K.D., Rane, M.R. et al. Assessment of genetic diversity in Mucuna species of India using randomly amplified polymorphic DNA and inter simple sequence repeat markers. Physiol Mol Biol Plants 22, 207–217 (2016). https://doi.org/10.1007/s12298-016-0361-3

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Keywords

  • DNA fingerprinting
  • Genetic diversity
  • ISSR
  • Mucuna
  • RAPD