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In vitro evaluation of excess copper affecting seedlings and their biochemical characteristics in Carthamus tinctorius L. (variety PBNS-12)

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Abstract

The present study was focused to recognize the changes in the Safflower (Carthamus tinctorius L. variety PBNS-12), when exposed to different concentration of copper (25, 50 and 100 μM) along with control (0.5 μM) for 10 and 20 days. This experiment used Hoagland’s nutrient solution to meet the external nutrient conditions, which includes micro and macronutrients equivalent to soil solution with copper sulphate (CuSO4. 5H2O) as a metal stress. The plant samples were harvested after 10 and 20 days. The effect of increased concentrations of copper was indicated by the reduction in overall growth with reduced fresh and dry weight. Copper stress caused significant increase in the non- enzymatic antioxidants (polyphenols and flavonoids) in leaves of treated safflower seedlings as compared to the control. Also, enhanced accumulation of proline was observed in the safflower leaves. In response to excess copper concentration, the level of MDA content was found to be increased. The results showed that the copper has time and dose-dependent effects on safflower seedlings.

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Abbreviations

ROS:

Reactive oxygen species

MDA:

Malondialdehyde

TI:

Tolerance Index

FW:

Fresh weight

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Acknowledgment

Authors are thankful to Prof. Aditya Shastri, Hon. Vice Chancellor, Banasthali University, Banasthali (Rajasthan), India for providing facilities for experimental work. Also we are thankful to Directorate of Oilseed Research (ICAR), Rajendranagar, Hyderabad (A.P.) India, for providing seeds to conduct this study.

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Authors and Affiliations

  1. Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, 304022, India

    Sanskriti Gautam & Nidhi Srivastava

  2. Directorate of Oilseed Research Institute, Hyderabad, India

    K. Anjani

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  1. Sanskriti Gautam
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  2. K. Anjani
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  3. Nidhi Srivastava
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Correspondence to Nidhi Srivastava.

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Gautam, S., Anjani, K. & Srivastava, N. In vitro evaluation of excess copper affecting seedlings and their biochemical characteristics in Carthamus tinctorius L. (variety PBNS-12). Physiol Mol Biol Plants 22, 121–129 (2016). https://doi.org/10.1007/s12298-016-0339-1

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  • DOI: https://doi.org/10.1007/s12298-016-0339-1

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