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Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers

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Abstract

Iran has a long history of acid lime cultivation and propagation. In this study, genetic variation in 28 acid lime accessions from five regions of south of Iran, and their relatedness with other 19 citrus cultivars were analyzed using Simple Sequence Repeat (SSR) and Inter-Simple Sequence Repeat (ISSR) molecular markers. Nine primers for SSR and nine ISSR primers were used for allele scoring. In total, 49 SSR and 131 ISSR polymorphic alleles were detected. Cluster analysis of SSR and ISSR data showed that most of the acid lime accessions (19 genotypes) have hybrid origin and genetically distance with nucellar of Mexican lime (9 genotypes). As nucellar of Mexican lime are susceptible to phytoplasma, these acid lime genotypes can be used to evaluate their tolerance against biotic constricts like lime “witches’ broom disease”.

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Authors and Affiliations

  1. Biotechnology Incubator Center of National Institute of Genetic Engineering and Biotechnology, Novin Giti Gene Biotech. Company (NGene Biotech. Co.), P. O. Box 1417863171, Tehran, Iran

    Ata Allah Sharafi

  2. Branch of North Region of Iran, Agricultural Biotechnology Research Institute of Iran (ABRII), P. O. Box 41635-4115, Rasht, Iran

    Ata Allah Sharafi, Asad Asadi Abkenar & Mohammad Masaeli

  3. Zanjan Pharmaceutical Biotechnology Research Center, School of Pharmacy, Zanjan University of Medical Sciences, P. O. Box 45195-1338, Zanjan, Iran

    Ali Sharafi

  4. Pharmaceutical Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran

    Ali Sharafi

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  1. Ata Allah Sharafi
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  2. Asad Asadi Abkenar
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  3. Ali Sharafi
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  4. Mohammad Masaeli
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Correspondence to Ali Sharafi.

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Sharafi, A.A., Abkenar, A.A., Sharafi, A. et al. Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers. Physiol Mol Biol Plants 22, 87–95 (2016). https://doi.org/10.1007/s12298-016-0336-4

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  • DOI: https://doi.org/10.1007/s12298-016-0336-4

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