Skip to main content

Advertisement

SpringerLink
Log in

Improving key enzyme activities and quality of rice under various methods of zinc application

  • Research Article
  • Published:
Physiology and Molecular Biology of Plants Aims and scope Submit manuscript

Abstract

Zinc (Zn) is an important micronutrient for the physiology of plants. It is poorly available to the plants in soil solution. A pot experiment was conducted to evaluate effectiveness of various Zn application methods on key enzyme activities and protein content of two contrasting rice genotypes viz., PD16 (Zn efficient) and NDR359 (Zn inefficient). The treatments were, control (0 mg Zn kg−1 soil), soil application (5 mg Zn kg−1 soil), foliar application (0.5 % ZnSO4 + 0.25 % lime at 30, 60 and 90 days after transplanting), soil (5 mg Zn kg−1 soil) + foliar application of 0.5 % ZnSO4 + 0.25 % lime at 30, 60 and 90 days after transplanting. Among all the methods tested soil+foliar application of Zn fertilizers was found most effective in increasing superoxide dismutase (SOD) and carbonic anhydrase (CA) activities as well as chlorophyll and protein content in both the rice varieties. NDR359, showed higher enzyme activities and more chlorophyll content in leaves than PD16, when Zn was applied either through foliar spray alone or in soil along with foliar application. Regarding the protein content in grains, PD16 showed higher protein content than NDR359, thus showed better translocation of Zn from leaves to grains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Arif M, Shehzad MA, Bashir F, Tasneem M, Yasin G, Iqbal M (2012) Boron, zinc and microtone effects on growth, chlorophyll contents and yield attributes in rice (Oryza sativa L.) cultivar. Afr J Biotechnol 11:10851–10858

    CAS  Google Scholar 

  • Bal HB, Nayak L, Das S, Adhya TK (2012) Isolation of ACC deaminase producing PGPR from rice rhizosphere and evaluating their plant growth promoting activity under salt stress. Plant Soil. doi:10.1007/s11104-012-1402-5

    Google Scholar 

  • Bharti K, Pandey N, Shankhdhar D, Srivastava PC, Shankhdhar SC (2013) Effect of different zinc levels on activity of superoxide dismutases & acid phosphatases and organic acid exudation on wheat genotypes. Physiol Mol Biol Plants. doi:10.1007/s12298-0130201-7

    PubMed Central  PubMed  Google Scholar 

  • Cakmak I (2002) Plant nutrition research: priorities to meet human needs for food in sustainable ways. Plant Soil 247:3–24

    Article  CAS  Google Scholar 

  • Cakmak I (2008) Enrichment of cereal grains with zinc: agronomic or genetic biofortification? Plant Soil 302:1–17

    Article  CAS  Google Scholar 

  • Cakmak I, Kalayci M, Ekizc H, Braund HJ, Kilinc Y, Yilmazc A, Lisbeth B, Meyer AS, Rasmussen SK (1999) Zinc deficiency as a practical problem in plants and human nutrition in Turkey: a NATO-science for stability project. Field Crops Res 60:175–188

    Article  Google Scholar 

  • Chen W, Yang X, He Z, Feng Y, Hu F (2008) Differential changes in photosynthetic capacity, chlorophyll fluorescence and chloroplast ultrastructure between Zn-efficient and Zn-inefficient rice (Oryza sativa L.) genotypes under low zinc stress. Physiol Plant 132:89–101

    Article  CAS  PubMed  Google Scholar 

  • Everson RG, Slack CR (1968) Distribution of carbonic anhydrase in relation to the C4 pathway of photosynthesis. Phytochemistry 7:581–584

    Article  CAS  Google Scholar 

  • Giannopolitis CN, Ries SK (1977) Superoxide dismutases. I. Occurrence in higher plants. Plant Physiol 59:309–314

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gibson TS, Leece DR (1981) Estimation of physiologically active zinc in maize by biochemical assay. Plant Sci 146:241–250

    Google Scholar 

  • Hacisalihoglu G, Hart JJ, Wang YH, Cakmak I, Kochian LV (2003) Zinc efficiency is correlated with enhanced expression and activity of zinc-requiring enzymes in wheat. Plant Physiol 131:595–602

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Hansch R, Mendel RR (2009) Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Curr Opin Plant Biol 12:259–266

    Article  PubMed  Google Scholar 

  • Herschfinkel M, Silverman WF, Sekler I (2007) The zinc sensing receptor, a link between zinc and cell signaling. Mol Med 13:331–336

    Google Scholar 

  • Hiscox JD, Israelstam GF (1979) A method for extraction of chlorophyll from leaf tissues without maceration. Can J Bot 57:1332–1334

    Article  CAS  Google Scholar 

  • Maret W (2009) Molecular aspects of human cellular zinc homeostasis: redox control of zinc potentials and zinc signals. Biometals 22:149–157

    Article  CAS  PubMed  Google Scholar 

  • Millan AFL, Ellis DR, Grusak MA (2005) Effect of zinc and manganese supply on the activities of superoxide dismutase and carbonic anhydrase in Medicago truncatula wild type and raz mutant plants. Plant Sci 168:1015–1022

    Article  Google Scholar 

  • Mirzapour MH, Khoshgoftar AH (2006) Zinc application effects on yield and seed oil content of sunflower grown on soil. J Plant Nutr 29:1719–1727

    Article  CAS  Google Scholar 

  • Morgounov A, Gomez-Becerra HF, Abugalieva A, Dzhunusova M, Yessimbekova M, Muminzanov H, Zelensky Y, Ozturk L, Cakmak I (2007) Iron and zinc grain density in common wheat grown in Central Asia. Euphytica 155:193–203

    Article  Google Scholar 

  • Morshedi A, Farahbakhshb H (2010) Effects of potassium and zinc on grain protein contents and yield of two wheat genotypes under soil and water salinity and alkalinity stresses. Plant Ecophysiol 2:67–72

    Google Scholar 

  • Muthukumararaja T, Sriramachandrasekharan MV (2012) Critical limit of zinc for rice soils of veeranam command area, Tamilnadu, India. ARPN J Agric Biol Sci 7(1):23–34

    CAS  Google Scholar 

  • Reza MIH, Chowdhury AQ, Pasha MK (2005) Characterization of proteins of brown, bran and endosperm of raw and parboiled rice. Res J Agric Biol Sci 1(2):184–189

    Google Scholar 

  • Rickli EE, Ghazanfar SAS, Gibbson BH, Edsall JT (1964) Carbonic anhydrases from human erythrocytes. J Biol Chem 239:1065–1078

    CAS  PubMed  Google Scholar 

  • Roy S, Duttay S, Khanna K, Singla S, Sundar D (2012) Prediction of DNA-binding specificity in zinc finger proteins. J Biosci 37(3):483–491

    Article  CAS  PubMed  Google Scholar 

  • Roy PD, Narwal RP, Malik RS, Saha BN, Kumar S (2014) Impact of zinc application methods on green gram (Vigna radiata L.) productivity and grain zinc fertilization. J Environ Biol 35:851–854

    CAS  PubMed  Google Scholar 

  • Sharma A, Babita P, Shankhdhar D, Shankhdhar SC (2015) Evaluation of different PGPR strains for yield enhancement and higher Zn content in different genotypes of rice (Oryza sativa L.). J Plant Nutr 38(3):456–472

    Article  CAS  Google Scholar 

  • Sinclair SA, Kraemer U (2012) The zinc homeostasis network of land plants. Biochim Biophys Acta Mol Cell Res 1823(9):1553–1567

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The financial assistance provided by NAIP, ICAR, New Delhi, during the period of this study is duly acknowledged.

Author information

Authors and Affiliations

  1. Department of Plant Physiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, 263145, Uttarakhand, India

    Bhupendra Mathpal, Deepti Shankhdhar & Shailesh Chandra Shankhdhar

  2. Department of Soil Science, College of Agriculture, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, 263145, India

    Prakash Chandra Srivastava

Authors
  1. Bhupendra Mathpal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Prakash Chandra Srivastava
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Deepti Shankhdhar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shailesh Chandra Shankhdhar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shailesh Chandra Shankhdhar.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mathpal, B., Srivastava, P.C., Shankhdhar, D. et al. Improving key enzyme activities and quality of rice under various methods of zinc application. Physiol Mol Biol Plants 21, 567–572 (2015). https://doi.org/10.1007/s12298-015-0321-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12298-015-0321-3

Keywords

Search

Navigation