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Direct adventitious shoot bud formation on hypocotyls explants in Millettia pinnata (L.) Panigrahi- a biodiesel producing medicinal tree species

Physiology and Molecular Biology of Plants volume 21, pages 287–292 (2015)Cite this article

Abstract

A reproducible protocol developed for in vitro regeneration of Milletia pinnata using hypocotyl segments. Multiple shoots were induced from hypocotyl explants through direct adventitious shoot bud regeneration. The proximal end of hypocotyls was responsive for shoot bud induction. Silver nitrate and adenine sulphate had a positive effect on shoot bud induction and elongation. The maximum response and number of shoot bud produced in media supplemented with 8.88 μM BAP with 108.6 μM adenine sulphate and 11.84 μM silver nitrate. Elongated shoots were harvested and successful rooting of microshoots achieved on MS media supplemented with 9.84 μM IBA, with 81.1 % rooting. Remaining shoot buds sub-cultured for further multiplication and elongation. Each subculture produced eight to nine elongated microshoots up to four subcultures. The rooted microshoots were successfully hardened and transferred to field.

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Acknowledgments

SKJ acknowledges the NOVOD board, Government of India for providing financial help. All authors are thankful to Dean, ASPEE College of Horticulture & Forestry, NAU, Navsari, and Director of Research, NAU, Navsari for extending facility and support.

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Affiliations

  1. Department of Forest Biology and Tree Improvement, College of Forestry, Navsari Agricultural University, Navsari, Gujarat, 396 450, India

    Durga Singh Nagar, Suman Kumar Jha & Jigar Jani

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  1. Durga Singh Nagar
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  2. Suman Kumar Jha
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  3. Jigar Jani
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Correspondence to Suman Kumar Jha.

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Nagar, D.S., Jha, S.K. & Jani, J. Direct adventitious shoot bud formation on hypocotyls explants in Millettia pinnata (L.) Panigrahi- a biodiesel producing medicinal tree species. Physiol Mol Biol Plants 21, 287–292 (2015). https://doi.org/10.1007/s12298-015-0293-3

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  • DOI: https://doi.org/10.1007/s12298-015-0293-3

Keywords

  • In vitro
  • Hypocotyl
  • Direct adventitious bud
  • Silver nitrate
  • Adenine sulphate
  • Milletia pinnata