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Antioxidants from defatted Indian Mustard (Brassica Juncea) protect biomolecules against in vitro oxidation

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Abstract

Indian mustard seeds were defatted by distillation with hexane and the residue extracted with methanol was analyzed for potential antioxidants; ascorbate, riboflavin, and polyphenols. Gallic acid (129.796 μg), caffeic acid (753.455 μg), quercetin (478.352 μg) and kaempferol (48.060 μg)/g dry seeds were identified by HPLC analysis of the extract. DPPH free radical scavenging activity and protection of lipids, proteins and DNA against metal induced oxidation was examined. Defatted mustard seed remnant had excellent free radical scavenging activity and protects biomolecules with IC50 value 2.0–2.25 mg dry seed weight. Significant content of polyphenols in methanol extract of defatted seeds accounts for high antioxidant potential. We are the first to report the detailed analysis of antioxidant composition and protection of biomolecules against oxidative damage by methanol extract of mustard seed remnant after oil extraction.

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Acknowledgements

The authors thank Dr. Dhiraj Singh, Head-Oil Seed Section, CCS Haryana Agricultural University, Hisar, India, for providing the seed material.

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Authors and Affiliations

  1. Department of Biochemistry, University College, Kurukshetra University, Kurukshetra, India

    Anita Dua

  2. Department of Genetics and Plant Breeding, Oil Seed Section, CCS Haryana Agricultural University, Hisar, India

    Subhash Chander

  3. Department of Biotechnology, Kurukshetra University, Kurukshetra, India

    Sharad Agrawal & Ritu Mahajan

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  1. Anita Dua
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  2. Subhash Chander
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  3. Sharad Agrawal
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  4. Ritu Mahajan
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Correspondence to Anita Dua.

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Dua, A., Chander, S., Agrawal, S. et al. Antioxidants from defatted Indian Mustard (Brassica Juncea) protect biomolecules against in vitro oxidation. Physiol Mol Biol Plants 20, 539–543 (2014). https://doi.org/10.1007/s12298-014-0260-4

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  • DOI: https://doi.org/10.1007/s12298-014-0260-4

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