Physiology and Molecular Biology of Plants

, Volume 20, Issue 2, pp 151–159

Arabidopsis mutant plants with diverse defects in polyamine metabolism show unequal sensitivity to exogenous cadaverine probably based on their spermine content

  • Taibo Liu
  • Hayato Dobashi
  • Dong Wook Kim
  • G. H. M. Sagor
  • Masaru Niitsu
  • Thomas Berberich
  • Tomonobu Kusano
Research Article

DOI: 10.1007/s12298-014-0227-5

Cite this article as:
Liu, T., Dobashi, H., Kim, D.W. et al. Physiol Mol Biol Plants (2014) 20: 151. doi:10.1007/s12298-014-0227-5

Abstract

Arabidopsis plants do not synthesize the polyamine cadaverine, a five carbon-chain diamine and structural analog of putrescine. Mutants defective in polyamine metabolic genes were exposed to exogenous cadaverine. Spermine-deficient spms mutant grew well while a T-DNA insertion mutant (pao4-1) of polyamine oxidase (PAO) 4 was severely inhibited in root growth compared to wild type (WT) or other pao loss-of-function mutants. To understand the molecular basis of this phenomenon, polyamine contents of WT, spms and pao4-1 plants treated with cadaverine were analyzed. Putrescine contents increased in all the three plants, and spermidine contents decreased in WT and pao4-1 but not in spms. Spermine contents increased in WT and pao4-1. As there were good correlations between putrescine (or spermine) contents and the degree of root growth inhibition, effects of exogenously added putrescine and spermine were examined. Spermine mimicked the original phenomenon, whereas high levels of putrescine evenly inhibited root growth, suggesting that cadaverine-induced spermine accumulation may explain the phenomenon. We also tested growth response of cadaverine-treated WT and pao4-1 plants to NaCl and found that spermine-accumulated pao4-1 plant was not NaCl tolerant. Based on the results, the effect of cadaverine on Arabidopsis growth and the role of PAO during NaCl stress are discussed.

Keywords

Arabidopsis Cadaverine NaCl response Polyamine oxidase Putrescine Spermidine Spermine 

Abbreviations

ACL5

T-Spm synthase

ADC

Arginine decarboxylase

Cad

Cadaverine

PAO

Polyamine oxidase

PAs

Polyamines

Put

Putrescine

SAMDC

S-adenosylmethionine decarboxylase

Spd

Spermidine

SPDS

Spd synthase

Spm

Spermine

SPMS

Spm synthase

T-Spm

Thermospermine

Supplementary material

12298_2014_227_Fig9_ESM.jpg (126 kb)
Figure S1

Schematic structure of AtPAO4 gene and the T-DNA insertion site in SALK_133599 line. A. The position of T-DNA insertion of pao4-1 (SALK_133599) is shown as a white rectangle. Box, exon; line, intron; white box, untranslated region; black box, coding region. B. Transcript levels of AtPAO4 in WT (Col-0) and pao4-1 plants. AtPAO4 transcripts were detected by qRT-PCR using total RNAs extracted from 14-day-old seedlings and normalized using CBP20 as an internal control. (JPEG 126 kb)

12298_2014_227_MOESM1_ESM.docx (16 kb)
Table S1The primers used for qRT-PCR analysis. (DOCX 16 kb)

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2014

Authors and Affiliations

  • Taibo Liu
    • 1
  • Hayato Dobashi
    • 1
  • Dong Wook Kim
    • 1
  • G. H. M. Sagor
    • 1
  • Masaru Niitsu
    • 2
  • Thomas Berberich
    • 3
  • Tomonobu Kusano
    • 1
  1. 1.Graduate School of Life SciencesTohoku UniversitySendaiJapan
  2. 2.Faculty of Pharmaceutical SciencesJosai UniversitySakadoJapan
  3. 3.Biodiversity and Climate Research Center (BiK-F)Frankfurt am MainGermany

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