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Isolation of a fruit ripening-related tonoplast aquaporin (GjTIP) gene from Gardenia jasminoides

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Abstract

Aquaporins are membrane water channels that play critical roles in controlling the water content of cells and tissues. In this work, a full-length cDNA encoding putative aquaporins was isolated from Gardenia jasminoides fruit cDNA library. The GjTIP cDNA is 1188 bp, contains a predicted 774 bp open reading frame that encodes 257 amino acids. A phylogenetic analysis conducted with previously characterized aquaporins from other plant species indicates that the cDNA encode putative tonoplast aquaporins (TIPs), and proposed that GjTIP has a tendency to be a mixed function aquaporin similar to the TIP1s from Arabidopsis and Gossypium raimondii. A typical “hourglasses” three-dimensional model of GjTIP was built. The expression of the GjTIP transcripts at fruits during maturation was conducted by RT-PCR analysis. The data revealed that the transcript levels of GjTIP have increased during fruit maturation.

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  1. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, Peoples Republic of China

    Lan Gao & Yi-jun Guo

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  1. Lan Gao
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  2. Yi-jun Guo
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Correspondence to Lan Gao.

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Gao, L., Guo, Yj. Isolation of a fruit ripening-related tonoplast aquaporin (GjTIP) gene from Gardenia jasminoides . Physiol Mol Biol Plants 19, 555–561 (2013). https://doi.org/10.1007/s12298-013-0191-5

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