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In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.)

Physiology and Molecular Biology of Plants volume 19, pages 261–268 (2013)Cite this article

Abstract

A system for in vitro selection of drought tolerant callus lines in sugarcane was developed. High molecular weight PEG was used as selective agent. Selected callus line grew better than non-selected callus when grown on different concentrations of PEG. The activity of antioxidant enzymes like CAT, POX, APX and SOD were high in selected callus than in non-selected callus. Osmolytes like proline and ascorbic acid were at higher levels in selected callus than in non-selected callus, however at higher concentrations (20–30 %) of PEG, levels of proline and ascorbic acid decreased. The frequency of organogenesis and number of plantlets decreased in selected callus than in non-selected callus. The results can be used for in vitro screening and manipulations of sugarcane for improvement of drought tolerance

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Fig. 1
Fig. 2

Abbreviations

2,4-D:

2,4-dichloro phenoxy acitic acid

BAP:

6-Benzyl aminopurin

NAA:

α-Napthalene acitic acid

Kn:

Kinetin

CAT:

Catalase

POX:

Peroxidase

APX:

Ascorbate peroxidase

SOD:

Superoxide dismutase

MS:

Murashige and Skoog

ROS:

Reactive Oxygen Species

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Acknowledgements

The authors thank head department of Botany Gulbarga University Gulbarga for the facilities provided during the course of the investigation,

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  1. Department of Botany, Gulbarga University, Gulbarga, India

    Srinath Rao & Jabeen FTZ

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  1. Srinath Rao
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  2. Jabeen FTZ
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Correspondence to Srinath Rao.

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Rao, S., FTZ, J. In vitro selection and characterization of polyethylene glycol (PEG) tolerant callus lines and regeneration of plantlets from the selected callus lines in sugarcane (Saccharum officinarum L.). Physiol Mol Biol Plants 19, 261–268 (2013). https://doi.org/10.1007/s12298-013-0162-x

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  • DOI: https://doi.org/10.1007/s12298-013-0162-x

Keywords

  • Antioxidant enzymes
  • Callus
  • PEG
  • Selection
  • Proline
  • Sugarcane