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Effects of dual stress (high salt and high temperature) on the photochemical efficiency of wheat leaves (Triticum aestivum)

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Abstract

In this study, we have focused on those components of Photosystem (PS) II which are significantly affected by dual stress (high salt and temperature) on wheat as measured by Plant Efficiency Analyser (PEA). It was observed that some of the chlorophyll a fluorescence parameters were temperature dominated, while some other parameters were salt dominated. We have also observed additive effects for parameters like antenna size heterogeneity. An important observation was that in high temperature alone, the K-step was observed at 40 °C, while in case of dual stress, the K-step was observed at 45 °C, while the Chl a fluorescence transient of 40 °C + 0.5 M NaCl was quite similar to 35 °C transient curve. In the presence of salt, K-step was observed at higher temperature suggesting a protection of OEC by salt. Plants are under dual stress, but effect of temperature stress is less severe in presence of salt stress. Thus, we can say that salt stress caused partial prevention from high temperature stress but it did not cause complete protection of PS II.

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Abbreviations

ABS:

Absorbance

Chl:

Chlorophyll

CSo:

Cross section of tested sample at t = 0, proportional to Fo

DCMU:

3-(3, 4-dichlorophenyl)-1, 1-dimethylurea

DIo/RC:

Dissipation per reaction center

ETo/RC:

Electron transport per reaction center

Fm:

Maximal Chl a fluorescence

Fo:

Minimal Chl a fluorescence

FR:

Fluorescence rise

Fv = Fm-Fo:

Variable fluorescence

K, J, I:

Intermediate steps of Chl a fluorescence rise between Fo and P

LHC:

Light harvesting complex

OEC:

Oxygen evolving complex

PEA:

Plant efficiency analyzer

PI:

Performance index

PS II:

Photosystem II

PQ:

Plastoquinone

QA :

Primary plastoquinone

QB :

Secondary plastoquinone

RC:

Reaction center

TRo/RC:

Trapping per reaction center

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Acknowledgement

Financial support for the project (INT/RFBR/P-98) to AJ by Department of Science and Technology (DST), New Delhi, India is thankfully acknowledged. SM thanks Council of Scientific and Industrial Research (CSIR), India for the Senior Research Fellowship [09/301/(0119)/2010/EMR-I]. PM thanks Council of Scientific and Industrial Research (CSIR), India for the Senior Research Fellowship [09/301/(0019)09/EMR-I]. We are also thankful to Prof. Reto J. Strasser and Ronaldo Maldonado-Rodriguez for gifting Biolyzer HP 3 Software.

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  1. School of Life Science, Devi Ahilya University, Indore, 452017, Madhya Pradesh, India

    Sonal Mathur, Pooja Mehta & Anjana Jajoo

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  1. Sonal Mathur
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  2. Pooja Mehta
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  3. Anjana Jajoo
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Correspondence to Anjana Jajoo.

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Mathur, S., Mehta, P. & Jajoo, A. Effects of dual stress (high salt and high temperature) on the photochemical efficiency of wheat leaves (Triticum aestivum). Physiol Mol Biol Plants 19, 179–188 (2013). https://doi.org/10.1007/s12298-012-0151-5

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