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Physiology and Molecular Biology of Plants

, Volume 18, Issue 4, pp 381–386 | Cite as

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

  • Seemab Mukhtar
  • Naseem Ahmad
  • Md Imran Khan
  • Mohammad Anis
  • Ibrahim M. Aref
Short Communication

Abstract

A comparative performance of two explants types (CN and Nodal) for their efficiency to induce multiple shoot regeneration in Clitoria ternatea has been carried out. Thidiazuron (TDZ) in different concentrations (0.05–2.5 μM) was used as a supplement to the Murashige and Skoog’s (MS) basal media. Explant type apart, two factors viz. concentration and exposure duration to TDZ played an important role in affecting multiple shoot regeneration. Cotyledonary node explants produced the best results at 0.1 μM TDZ, while in nodal explants the highest rate of shoot formation was achieved on MS medium supplemented with 1.0 μM TDZ. In both the explants, shoot multiplication increased when the regenerated shoots were subcultured on hormone free MS medium after 4 weeks of exposure to TDZ. Among the two, cotyledonary node explants produced considerably higher number of shoots at a comparatively lower concentration of TDZ than nodal explants. The regenerated shoots rooted best on MS medium containing 1.0 μM indole-3-butyric acid (IBA) and were successfully established in pots containing garden soil with 88 % survival rate. All the regenerated plants showed normal morphology and growth characteristics.

Keywords

Thidiazuron Acclimatization Butterfly pea Cotyledonary node Node 

Notes

Acknowledgements

Authors appreciate the financial assistance provided by the University Grants Commission, Govt. of India, under UGC-SAP (DRS-I) program (2009) and Department of Science and Technology (DST) Govt. of India, in the form of DST-FIST program (2011) to the Department of Botany, Aligarh Muslim University.

References

  1. Ahmad N, Anis M (2007) Rapid plant regeneration protocol for cluster bean (Cyamopsis tetragonoloba L. Taub.). J Hort Sci Biotechnol 82:585–589Google Scholar
  2. Ahmad N, Siddique I, Anis M (2006) Improved plant regeneration in Capsicum annuum from nodal segment. Biol Plant 50:701–704CrossRefGoogle Scholar
  3. Anonymous (1988) The wealth of India: a dictionary of Indian raw materials and industrial products. Vol. II. New Delhi: Publication and Information Directorate. CSIRGoogle Scholar
  4. Barik DP, Naik SK, Mudgal A, Chand PK (2007) Rapid plant regeneration through in vitro axillary shoot proliferation of butterfly pea (Clitoria ternatea L.)—a twinning legume. In Vitro Cell Dev Biol Plant 43:144–148CrossRefGoogle Scholar
  5. Capelle SC, Mok DWS, Kirchner SC, Mok MC (1983) Effects of thidiazuron on cytokinin autonomy and the metabolism of N6 (2-isopentenyl)[8-'4C]adenosine in callus tissue of Phaseolus lunatus L. Plant Physiol 73:796–802CrossRefPubMedPubMedCentralGoogle Scholar
  6. Eapen S, Tivarekar S, George L (1998) Thidiazuron induced shoot regeneration in pigeon pea (Cajanus cajan L.). Plant Cell Tissue Organ Cult 53:217–220CrossRefGoogle Scholar
  7. Faisal M, Anis M (2006) Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia. Biol Plant 50:437–440CrossRefGoogle Scholar
  8. Faisal M, Ahmad N, Anis M (2005) Shoot multiplication in Rauvolfia tetraphylla L. using thidiazuron. Plant Cell Tissue Organ Cult 80:187–190CrossRefGoogle Scholar
  9. Faisal M, Siddique I, Anis M (2006) In vitro rapid regeneration of plantlets from nodal explants of Mucuna pruriens—a valuable medicinal plant. Ann Appl Biol 148:1–6CrossRefGoogle Scholar
  10. Fracaro F, Echiverrigaray S (2001) Micropropagation of Cunila galioides, a popular medicinal plant of South Brazil. Plant Cell Tissue Organ Cult 64:1–4CrossRefGoogle Scholar
  11. Gomez SM, Kalamani A (2003) Butterfly pea (Clitoria ternatea): a nutritive multipurpose forage legume for the tropics—an overview. Pak J Nutr 2:374–379CrossRefGoogle Scholar
  12. Gubiš J, Lajchová Z, Faragó J, Jureková Z (2003) Effect of genotype and explant type on shoot regeneration in tomato (Lycopersicon esculentum Mill.) in vitro. Czech J Genet Plant Breed 39:9–14Google Scholar
  13. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119CrossRefGoogle Scholar
  14. Ishag S, Osman MG, Khalafalla MM (2009) Effects of growth regulators, explant and genotype on shoot regeneration in tomato (Lycopersicon esculentum c.v. Omdurman). Int J Sustain Crop Prod 4:7–13Google Scholar
  15. Ismail N, Rani U, Batra A (2011) Crucial role of TDZ in the quick regeneration of multiple shoots of Clitoria ternatea L. Int J Pharma Sci Rev Res 6:23–26Google Scholar
  16. Jain MN, Dhal CC, Shroff RH, Bhutada RH, Somani RS, Kasture VS, Kasture SB (2003) Clitoria ternatea and the CNS. Pharmacol Biochem Behav 75:529–536CrossRefPubMedGoogle Scholar
  17. Kanyand N, Dessai AP, Prakash SC (1994) Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogea) plants in vitro. Plant Cell Rep 14:1–5CrossRefPubMedGoogle Scholar
  18. Khan MI, Ahmad N, Anis M (2011) The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.: a tree of ecological importance. Trees 25:577–584CrossRefGoogle Scholar
  19. Lakshmanan KK, Dhanalakshmi S (1990) Callus, organogenesis and plantlet formation in tissue cultures of Clitoria ternatea. Ann Bot 66:451–455Google Scholar
  20. Malabadi RB, Nataraja K (2001) Shoot regeneration in leaf explants of Clitoria ternatea L. cultured in vitro. Phytomorphology 51:169–171Google Scholar
  21. Malik KA, Saxena PK (1992) Regeneration in Phaseolus vulgaris L. high frequency induction of direct shoot formation in intact seedlings by N6-benzylamino purine and thidiazuron. Planta 186:384–389CrossRefPubMedGoogle Scholar
  22. Mohamed N, Taha RM (2011) Plant regeneration of Clitoria ternatea from leaf explants cultured in vitro. J Food Agric Environ 9:268–270Google Scholar
  23. Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamoto T (1982) Cytokinin activity of N-phenyl-N’-1,2,3-thidiazol-5-ylurea (thidiazuron). Phytochemistry 21:1509–1511CrossRefGoogle Scholar
  24. Morris JB (1999) Legume genetic resources with novel ‘value added’ industrial and pharmaceutical use. In: Janick J (ed) Perspectives on new crops and new uses. ASHS, Alexandria, pp 196–201Google Scholar
  25. Mukhtar S, Anis M, Ahmad N (2010) In vitro optimization of phytohormones on micropropagation in Butterfly pea (Clitoria ternatea L.). J Herbs Spices Med Plants 16:98–105CrossRefGoogle Scholar
  26. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tiossue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  27. Murch SJ, Saxena PK (2001) Molecular fate of thidiazuron and its effects on auxin transport in hypocotyls tissues of Pelargonium x hortorum Bailey. Plant Growth Regul 35:269–275CrossRefGoogle Scholar
  28. Panday NK, Tewari KC, Tewari RN, Joshi GC, Pande VN, Pandey G (1993) Medicinal plants of Kumaon Himalaya: strategies for conservation. In: Dhar U (ed) Himalayan biodiversity conservation strategies, no. 3. Himavikas, Nanital, pp 293–302Google Scholar
  29. Pattnaik S, Chand PK (1996) In vitro propagation of the medicinal herbs Ocimum americanum L. Syn. O. canum Sims. (Hoary bsil) and Ocimum sanctum L. (Holy basil). Plant Cell Rep 15:846–850CrossRefPubMedGoogle Scholar
  30. Rajeswari V, Paliwal K (2008) In vitro plant regeneration of red sanders (Pterocarpus santalinus L.f.) from cotyledonary nodes. Ind J Biotechnol 7:541–546Google Scholar
  31. Rout GR (2005) Micropropagation of Clitoria ternatea Linn. (Fabaceae): an important medicinal plant. In vitro Cell Dev Biol-Plant 41:516–519CrossRefGoogle Scholar
  32. Sanago MHM, Shataluk VI, Stromme J (1996) Rapid plant regeneration of pea using thidiazuron. Plant Cell Tissue Org Cult 45:165–168CrossRefGoogle Scholar
  33. Shahzad A, Faisal M, Anis M (2007) Micropropagation through excised root culture of Clitoria ternatea L. and comparison between in vitro regenerated plants and seedling. Ann Appl Biol 150:341–349CrossRefGoogle Scholar
  34. Siddique I, Anis M (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.)—a medicinal plant. Acta Physiol Plant 29:333–338CrossRefGoogle Scholar
  35. Singh J, Tiwari KN (2010) High-frequency in vitro multiplication system for commercial propagation of pharmaceutically important Clitoria ternatea L.—a valuable medicinal plant. Ind Crop Prod 32:534–538CrossRefGoogle Scholar
  36. Singh J, Tiwari KN (2012) In vitro plant regeneration from decapitated embryonic axes of Clitoria ternatea L.—an important medicinal plant. Ind Crop Prod 35:224–229CrossRefGoogle Scholar
  37. Sudha CG, Seeni S (1994) In vitro multiplication and field establishment Adhotoda beddomei GB, Clarke, a rare medicinal plant. Plant Cell Rep 13:203–207PubMedGoogle Scholar
  38. Tiwari V, Tiwari KN, Singh BD (2001) Comparitive studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue Org Cult 66:9–16CrossRefGoogle Scholar
  39. Victor JMR, Murthy BNS, Murch SJ, Krihnaraj S, Saxena PK (1999) Role of endogenous purine metabolism in thidiazuron-induced somatic embryogenesis of peanut (Arachis hypogea). Plant Growth Regul 28:41–47CrossRefGoogle Scholar

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2012

Authors and Affiliations

  • Seemab Mukhtar
    • 1
  • Naseem Ahmad
    • 1
  • Md Imran Khan
    • 1
  • Mohammad Anis
    • 1
    • 2
  • Ibrahim M. Aref
    • 2
  1. 1.Plant Biotechnology laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Plant Production, College of Food and Agriculture SciencesKing Saud UniversityRiyadhSaudi Arabia

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