Physiology and Molecular Biology of Plants

, Volume 18, Issue 4, pp 381–386 | Cite as

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

  • Seemab Mukhtar
  • Naseem Ahmad
  • Md Imran Khan
  • Mohammad AnisEmail author
  • Ibrahim M. Aref
Short Communication


A comparative performance of two explants types (CN and Nodal) for their efficiency to induce multiple shoot regeneration in Clitoria ternatea has been carried out. Thidiazuron (TDZ) in different concentrations (0.05–2.5 μM) was used as a supplement to the Murashige and Skoog’s (MS) basal media. Explant type apart, two factors viz. concentration and exposure duration to TDZ played an important role in affecting multiple shoot regeneration. Cotyledonary node explants produced the best results at 0.1 μM TDZ, while in nodal explants the highest rate of shoot formation was achieved on MS medium supplemented with 1.0 μM TDZ. In both the explants, shoot multiplication increased when the regenerated shoots were subcultured on hormone free MS medium after 4 weeks of exposure to TDZ. Among the two, cotyledonary node explants produced considerably higher number of shoots at a comparatively lower concentration of TDZ than nodal explants. The regenerated shoots rooted best on MS medium containing 1.0 μM indole-3-butyric acid (IBA) and were successfully established in pots containing garden soil with 88 % survival rate. All the regenerated plants showed normal morphology and growth characteristics.


Thidiazuron Acclimatization Butterfly pea Cotyledonary node Node 



Authors appreciate the financial assistance provided by the University Grants Commission, Govt. of India, under UGC-SAP (DRS-I) program (2009) and Department of Science and Technology (DST) Govt. of India, in the form of DST-FIST program (2011) to the Department of Botany, Aligarh Muslim University.


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2012

Authors and Affiliations

  • Seemab Mukhtar
    • 1
  • Naseem Ahmad
    • 1
  • Md Imran Khan
    • 1
  • Mohammad Anis
    • 1
    • 2
    Email author
  • Ibrahim M. Aref
    • 2
  1. 1.Plant Biotechnology laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Plant Production, College of Food and Agriculture SciencesKing Saud UniversityRiyadhSaudi Arabia

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