Physiology and Molecular Biology of Plants

, Volume 18, Issue 3, pp 229–236 | Cite as

Physiological and biochemical effect of 24-epibrassinoslide on cold tolerance in maize seedlings

  • Ishwar Singh
  • Upendra Kumar
  • S. K. Singh
  • Charu Gupta
  • Madhulika Singh
  • S. R. Kushwaha
Research Article


Germination and early seedling growth are important for establishment of maize because maize is chilling sensitive crop and low temperature during early period of growth can be detrimental to subsequent crop growth and productivity. Therefore, it is important to protect maize seedling from cold stress. A study was conducted on induced cold tolerance by 24-epibrassinoslide (EBR) at the Indian Agricultural Research Institute, New Delhi, India. Maize seedlings were raised in green house condition (25/18 °C day-night temperatures). Ten days old seedlings were treated with EBR (0.0, 0.01, 0.1, 1.0 and 10 μM) and then divided into two sets, one set was kept in greenhouse (25/18 °C day-night temperatures) and another was transferred to net house (cold stress). Data on various morpho-physiological traits was recorded after 7, 14 and 21 days of treatment. Exogenous application of 1.0 μM EBR had significant effect on growth and morpho-physiological traits under both conditions. The maize seedlings treated with EBR were more tolerant to cold stress than the untreated one. Significant increase in plant height, dry matter accumulation, chlorophyll content, total soluble proteins and starch contents was observed under both conditions, however, the results were more pronounced under cold stress. 1.0 μ M concentration being the most effective under both conditions. Maintenance of high tissue water content, reduced membrane injury index, increased total chlorophyll, soluble sugar and protein content were taken as the possible indicators of EBR induced chilling tolerance.


24-epibrassinoslide Cold tolerance Maize Glycine betaine Total chlorophyll Membrane injury index 


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2012

Authors and Affiliations

  • Ishwar Singh
    • 1
  • Upendra Kumar
    • 1
  • S. K. Singh
    • 1
  • Charu Gupta
    • 1
  • Madhulika Singh
    • 1
  • S. R. Kushwaha
    • 2
  1. 1.Directorate of Maize ResearchNew DelhiIndia
  2. 2.Division of plant physiologyIndian Agricultural Research InstituteNew DelhiIndia

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