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In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant


In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered species of Stemonaceae, was established. Nodal and internodal explants were cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) alone or in combination with 1-naphthaleneacetic acid (NAA). When cultured on MS medium supplemented with 18.16 μM TDZ with 0.54 μM NAA for 8 weeks and then on MS medium without plant growth regulator (PGR) for 8 weeks, nodal explants demonstrated a responding frequency of 91.67 % and a shoot regeneration rate of 5.46 shoots/responding explant. Furthermore, internodal explants demonstrated a responding frequency of 17.17 % with 11.17 shoots/responding explant. The regenerated shoots were rooted using a two-step protocol by culturing for 4 weeks on MS medium supplemented with various concentrations of NAA and Indole-3-butyric acid (IBA) and 8 weeks on MS medium without PGR. When 5.40 μM NAA was used as a PGR, the maximum root induction rate was 69.45 %. Regenerated plantlets were successfully transferred to soil.

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Indole-3-butyric acid

MS medium:

Murashige and Skoog (Murashige and Skoog 1962) medium


1-Naphthaleneacetic acid


Plant growth regulator




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Financial support from Mahidol University is gratefully acknowledged.

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Correspondence to Sompop Prathanturarug.

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Prathanturarug, S., Pheakkoet, R., Jenjittikul, T. et al. In vitro propagation of Stemona hutanguriana W.Chuakul, an endangered medicinal plant. Physiol Mol Biol Plants 18, 281–286 (2012).

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  • Conservation
  • Medicinal plant
  • Stemonaceae
  • Stemona hutanguriana
  • Tissue culture