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Micropropagation of Araucaria excelsa R. Br. var. glauca Carrière from orthotropic stem explants

Abstract

The objectives of the present work were in vitro propagation of Araucaria excelsa R. Br. var. glauca Carrière (Norfolk Island pine) with focus on the evaluation of the mean number of shoots per explant (MNS/E) and mean length of shoots per explants (MLS/E) produced by different parts of the orthotropic stem of A. excelsa R. Br. var. glauca in response to plant growth regulators. Norfolk Island pine axillary meristems responded very well to the 2-iso-pentenyl adenine (2iP) and thidiazuron (TDZ) levels. Explants taken from stem upper segments in the media containing 2iP had a higher MNS/E (3.47) and MLS/E (6.27 mm) in comparison to those taken from stem lower segments, which were 0.71 and 0.51 mm, respectively. Using 0.045 μM TDZ in the MS medium not only resulted in 4.60 MNS/E with 7.08 mm MLS/E but proliferated shoots showed a good performance as well. Investigating the best position of stem explant on mother plant as well as the best concentrations of growth regulators were performed which were useful for efficient micropropagation of this plant. Thirty three percent of explants were rooted in the MS medium containing 3 % sucrose, supplemented with 7.5 μM of both NAA and IBA for 2 weeks before transferring to a half strength MS medium without any growth regulator. Plantlets obtained were acclimatized and transferred to the greenhouse with less than 20 % mortality. This procedure considered the first successful report for regeneration and acclimatization of A. excelsa R. Br. var. glauca plantlet through main stem explants.

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Abbreviations

2iP:

2-iso-pentenyl adenine

AC:

Activated charcoal

BAP:

6-benzyl-amino-purine

IBA:

Indole-3-butyric acid

Kin:

Kinetin

MLS/E:

Mean length of shoots per explant

MNS/E:

Mean number of shoots per explant

MS:

Murashige and Skoog

NAA:

α-naphthaleneacetic acid

PGRs:

Plant growth regulators

TDZ:

Thidiazuron

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Correspondence to Mostafa Khoshhal Sarmast.

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Sarmast, M.K., Salehi, H. & Khosh-Khui, M. Micropropagation of Araucaria excelsa R. Br. var. glauca Carrière from orthotropic stem explants. Physiol Mol Biol Plants 18, 265–271 (2012). https://doi.org/10.1007/s12298-012-0115-9

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Keywords

  • Abnormality
  • Araucaria excelsa R. Br. var. glauca
  • Axillary meristems
  • Orthotropic stems
  • Proliferation
  • Topophysis