Physiology and Molecular Biology of Plants

, Volume 18, Issue 3, pp 253–263 | Cite as

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

  • Mohd. Zahid Rizvi
  • Arun Kumar KukrejaEmail author
  • Narendra Singh Bisht
Research Article


Efficient in vitro propagation of medicinally important endangered plant C. borivilianum has been achieved through somatic embryogenesis. Solid embryogenic medium [Murashige and Skoog medium containing 1.79 mM NH4NO3, 10.72 mM KNO3, 1.13 μM 2,4-dichlorophenoxyacetic acid, 7.38 μM 2-isopentenyladenine and 0.76 mM proline] supplemented with polyethylene glycol and sucrose (3 % each), exhibited 1.88-fold increase in embryo maturation compared to embryogenic medium containing 3 % sucrose. Liquid embryogenic medium supported better somatic embryo production and maturation. Highest total (79) and mature (cotyledonary stage) somatic embryos (38) as well as highest germination (57.5 %) was observed at inoculum density of 0.4 g/40 ml of liquid medium. 5.86 pH level exhibited optimal growth, maturation and germination of somatic embryos. Random amplified polymorphic DNA (RAPD) analysis of C. borivilianum plants regenerated through somatic embryogenesis revealed that they were genetically similar to the mother plant. The protocol established in the present study can be used for rapid mass multiplication of C. borivilianum in bioreactor employing liquid medium.


Chlorophytum borivilianum Genetic fidelity Maturation Polyethylene glycol Somatic embryos 



The necessary facilities provided by Director, Central Institute of Medicinal and Aromatic Plants and financial support of Council of Scientific and Industrial Research, India as Senior Research Fellowship to M. Z. Rizvi are duly acknowledged.


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2012

Authors and Affiliations

  • Mohd. Zahid Rizvi
    • 1
    • 3
  • Arun Kumar Kukreja
    • 1
    Email author
  • Narendra Singh Bisht
    • 2
  1. 1.Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants (CIMAP)LucknowIndia
  2. 2.Department of BotanyHemwati Nandan Bahuguna Garhwal University (Pauri Campus)Garhwal-246174India
  3. 3.Department of BotanyShia Post Graduate CollegeLucknow-226020India

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