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Physiology and Molecular Biology of Plants

, Volume 18, Issue 3, pp 253–263 | Cite as

RETRACTED ARTICLE: Plant regeneration in Chlorophytum borivilianum Sant. et Fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis

  • Mohd. Zahid Rizvi
  • Arun Kumar KukrejaEmail author
  • Narendra Singh Bisht
Research Article

Abstract

Efficient in vitro propagation of medicinally important endangered plant C. borivilianum has been achieved through somatic embryogenesis. Solid embryogenic medium [Murashige and Skoog medium containing 1.79 mM NH4NO3, 10.72 mM KNO3, 1.13 μM 2,4-dichlorophenoxyacetic acid, 7.38 μM 2-isopentenyladenine and 0.76 mM proline] supplemented with polyethylene glycol and sucrose (3 % each), exhibited 1.88-fold increase in embryo maturation compared to embryogenic medium containing 3 % sucrose. Liquid embryogenic medium supported better somatic embryo production and maturation. Highest total (79) and mature (cotyledonary stage) somatic embryos (38) as well as highest germination (57.5 %) was observed at inoculum density of 0.4 g/40 ml of liquid medium. 5.86 pH level exhibited optimal growth, maturation and germination of somatic embryos. Random amplified polymorphic DNA (RAPD) analysis of C. borivilianum plants regenerated through somatic embryogenesis revealed that they were genetically similar to the mother plant. The protocol established in the present study can be used for rapid mass multiplication of C. borivilianum in bioreactor employing liquid medium.

Keywords

Chlorophytum borivilianum Genetic fidelity Maturation Polyethylene glycol Somatic embryos 

Notes

Acknowledgements

The necessary facilities provided by Director, Central Institute of Medicinal and Aromatic Plants and financial support of Council of Scientific and Industrial Research, India as Senior Research Fellowship to M. Z. Rizvi are duly acknowledged.

References

  1. Alkhateeb AA (2006) Somatic embryogenesis in date palm (Phoenix dactylifera L) cv sukary in response to sucrose and polyethylene glycol. Biotech 5(4):466–470CrossRefGoogle Scholar
  2. Bennici A, Anzidei M, Vendramin GG (2004) Genetic stability and uniformity of Foeniculum vulgare Mill. regenerated plants through organogenesis and somatic embryogenesis. Plant Sci 166:221–227CrossRefGoogle Scholar
  3. Bindiya K, Kanwar K (2003) Random amplified polymorphic DNA (RAPDs) markers for genetic analysis in micropropagated plants of Robinia pseudoacacia L. Euphytica 132:41–47CrossRefGoogle Scholar
  4. Bordia PC, Joshi A, Simlot MM (1995) Safed musli. In: Chadha KL, Gupta R (eds) Advances in horticulture: medicinal and aromatic plants, vol 11. Malhotra Pub House, New Delhi, pp 429–451Google Scholar
  5. Cardoza V, D’Souza L (1999) Somatic embryogenesis and plantlet regeneration in Mussaenda philippica var Aurorae. Gartenbauwissenschaft 64(1):S6–S8Google Scholar
  6. Cecchini E, Natah L, Cavallini A, Durante M (1992) DNA variation in regenerated plants of pea. Theor Appl Genet 84:874–879PubMedGoogle Scholar
  7. Eapen S, George L (1990) Influence of phytohormones, carbohydrates, amino acids, growth supplements and antibiotics on somatic embryogenesis and plant differentiation in finger millet. Plant Cell Tissue Organ Cult 22:87–93CrossRefGoogle Scholar
  8. Elmeer KMS, Gallagher TF, Hennerty MJ (2009) RAPD-based detection of genomic instability in cucumber plants derived from somatic embryogenesis. African J Biotechnol 8(14):3219–3222Google Scholar
  9. Fujimura T, Komamine A (1979) Synchronization of somatic embryogenesis in a carrot suspension culture. Plant Physiol 64:162–164CrossRefPubMedPubMedCentralGoogle Scholar
  10. Gavish H, Vardi A, Fluhr R (1992) Suppression of somatic embryogenesis in Citrus cell cultures by extracellular proteins. Planta 186:511–517CrossRefPubMedGoogle Scholar
  11. Gerdakaneh M, Mozafari AA, Khalighi A, Sioseh-mardah A (2009) The effect of carbohydrate source and concentration on somatic embryogenesis of strawberry (Fragaria x ananassa Duch). Am Eurasian J Agric Environ Sci 6(1):76–80Google Scholar
  12. Gray DJ, Conger BV (1985) Influence of dicamba and casein hydrolysate on somatic embryo number and culture quality in cell suspensions of Dactylis glomerata (Gramineae). Plant Cell Tissue Organ Cult 4(2):123–133CrossRefGoogle Scholar
  13. Gupta R, Chadha KL (1995) Medicinal and aromatic plants research in India. In: Chadha RK, Gupta R (eds) Advances in horticulture, vol XI. Malhotra Pub House, New Delhi, pp 1–43Google Scholar
  14. Ho CW, Jian WT, Lai HC (2006) Plant regeneration via somatic embryogenesis from suspension cell cultures of Lilium x formolongi Hort using a bioreactor system. in vitro Cell Dev Biol Plant 42:240–246Google Scholar
  15. Hong PI, Chen JT, Chang WC (2008) Promotion of direct somatic embryogenesis of Oncidium by adjusting carbon sources. Biol Plant 52(3):597–600CrossRefGoogle Scholar
  16. Jayanthi M, Mandal PK (2001) Plant regeneration through somatic embryogenesis and RAPD analysis of regenerated plants in Tylophora indica (Burm F Merrill). in vitro Cell Dev Biol Plant 37:576–580Google Scholar
  17. Khanuja SPS, Shasanay AK, Darokar MP, Kumar S (1999) Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils. Plant Mol Biol Rep 17:74CrossRefGoogle Scholar
  18. Khanuja SPS, Shasany AK, Srivastava A, Kumar S (2000) Assessment of genetic relationships in Mentha species. Euphytica 111:121–125CrossRefGoogle Scholar
  19. Kim EK, Hahn EJ, Murthy HN, Paek KY (2004) Enhanced shoot and bulblet proliferation of garlic (Allium sativam L) in bioreactor systems. J Hortic Sci 79:818–822Google Scholar
  20. Kirtikar KR, Basu BD (1975) Liliaceae: Chlorophytum. In: Kirtikar KR, Basu BD (eds) Indian medicinal plants. Bishan Singh Mahendra Pal Singh, Dehradun, pp 2508–2509Google Scholar
  21. Kosky RG, de Feria Silva MD, Perez LP, Gilliard T, Martinez FB, Vega MR, Milian MC, Mendoza EQ (2002) Somatic embryogenesis of the banana hybrid cultivar FHIA-(AAAB) in liquid medium and scaled-up in a bioreactor. Plant Cell Tissue Organ Cult 68:21–26CrossRefGoogle Scholar
  22. Kumar N, Modi AR, Singh AS, Gajera BB, Patel AR, Patel MP, Subhash N (2010) Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L) plants by RAPD and ISSR markers assay. Physiol Mol Biol Plants 16(2):207–213CrossRefPubMedPubMedCentralGoogle Scholar
  23. Malabadi RB, da Silva JAT, Nataraja K, Kumar SV, Mulgund GS (2011) Induction of somatic embryogenesis in mature coniferous forest trees. Res Biotech 2(5):8–33Google Scholar
  24. Martin KP (2003) Plant regeneration through somatic embryogenesis on Holostemma adakodien, a rare medicinal plant. Plant Cell Tissue Organ Cult 72:79–82CrossRefGoogle Scholar
  25. Mitra A, Bhattacharaya PS, Dey S, Sawarker SK, Bhattacharayya BC (1998) Photoautotrophic in vitro culture of Chrysanthemum under CO2 enrichment. Biotechnol Tech 12:335–337Google Scholar
  26. Murashige T, Skoog FA (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  27. Nadha HK, Kumar R, Sharma RK, Anand M, Sood A (2011) Evaluation of clonal fidelity of in vitro raised plants of Guadua angustifolia Kunth using DNA-based markers. J Med Plants Res 5(23):5636–5641Google Scholar
  28. Nasir IA, Jahangir GZ, Qamar Z, Rahman Z, Hasnain T (2011) Maintaining the regeneration potential of sugarcane callus for longer span. Afr J Agric Res 6(1):113–119Google Scholar
  29. Nayar MP, Sastry ARK (1988) Chlorophytum borivilianum. In: Nayar MP, Sastry ARK (eds) Red data book of Indian plants, vol 2. Botanical survey of India, Calcutta, p 142Google Scholar
  30. Nhut DT, Vinh BVT, Hien TT, Huy NP, Nam NB, Chien HX (2011) Effects of spermidine, proline and carbohydrate sources on somatic embryogenesis from main root transverse thin cell layers of Vietnamese ginseng (Panax vietnamensis Ha et Grushv). Afr J Biotechnol 11(5):1084–1091Google Scholar
  31. Nomura K, Komamine A (1985) Identification and isolation of single cells that produce somatic embryos at a high frequency in a carrot suspension culture. Plant Physiol 79:988–991CrossRefPubMedPubMedCentralGoogle Scholar
  32. Othmani A, Bayoudh C, Drira N, Trifi M (2009) in vitro cloning of date palm Phoenix dactylifera L, cv Deglet Bey by using embryogenic suspension and temporary immersion bioreactor (TIB). Biotechnol Biotechnol Equip 23:1181–1188Google Scholar
  33. Paek KY, Chakrabarty D, Hahn EJ (2005) Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell Tissue Organ Cult 81:287–300CrossRefGoogle Scholar
  34. Patel S, Jasrai YT, Adiyecha R (2011) Induction of somatic embryogenesis and genetic fidelity of endangered medicinal herb Curculigo orchioides Gaertn. Res Plant Biol 1(3):48–52Google Scholar
  35. Peyvandi M, Farahzadi HN, Arbabian S, Noormohammadi Z, Hosseini-Mazinani M (2010) Somaclonal variation among somatic embryo derived plants of Olea europaea L “cv Kroneiki”. J IR Iran 21(1):7–14Google Scholar
  36. Purohit SD, Dave A, Kukda G (1994) Somatic embryogenesis and plantlet regeneration in ‘safed musli’ (Chlorophytum borivilianum). Indian J Plant Genet Resour 7(1):65–71Google Scholar
  37. Ramawat KG, Jain S, Suri SS, Arora DK (1988) Aphrodisiac plants of Aravalli Hills with special reference to safed musli. In: Khan I, Khanum A (eds) Role of biotechnology in medicinal and aromatic plants. Ukaz Pub, Hyderabad, pp 210–223Google Scholar
  38. Rani V, Raina SN (1998) Genetic analysis of enhanced axillary branching derived Eucalyptus tereticornis Smith and E. camaldulensis Dehn plants. Plant Cell Rep 17:236–242CrossRefGoogle Scholar
  39. Rani V, Parida A, Raina SN (1995) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoides Marsh. Plant Cell Rep 14:459–462CrossRefPubMedGoogle Scholar
  40. Rizvi MZ, Kukreja AK, Bisht NS (2010) in vitro propagation of an endangered medicinal herb Chlorophytum borivilianum Sant et Fernand through somatic embryogenesis. Physiol Mol Biol Plants 16(3):249–257Google Scholar
  41. Smith DL, Krikorian AD (1990) Somatic embryogenesis of carrot in hormone-free medium: external pH control over morphogenesis. Am J Bot 77:1634–1647CrossRefPubMedGoogle Scholar
  42. Tandon M, Shukla YN (1995) Phytoconstituents of Asparagus adscendens, Chlorophytum arundinaceum and Curculigo orchioides: a review. Curr Res Med Aromat Plants 17:42–50Google Scholar
  43. Tyagi RK, Agarwal A, Mahalakshmi C, Hussain Z, Tyagi H (2007) Low-cost media for in vitro conservation of turmeric (Curcuma longa L) and genetic stability assessment using RAPD markers. in vitro Cell Dev Biol Plant 43:51–58Google Scholar
  44. Walker DR, Parrott WA (2001) Effect of polyethylene glycol and sugar alcohols on soybean somatic embryo germination and conversion. Plant Cell Tissue Organ Cult 64:55–62CrossRefGoogle Scholar
  45. Williams JGK, Kubelick AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531CrossRefPubMedPubMedCentralGoogle Scholar
  46. Yang XM, An LZ, Xiong YC, Zhang JP, Li Y, Xu SJ (2008) Somatic embryogenesis from immature zygotic embryos and monitoring the genetic fidelity of regenerated plants in grapevine. Biol Plant 52:209–214CrossRefGoogle Scholar

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2012

Authors and Affiliations

  • Mohd. Zahid Rizvi
    • 1
    • 3
  • Arun Kumar Kukreja
    • 1
    Email author
  • Narendra Singh Bisht
    • 2
  1. 1.Plant Tissue Culture Division, Central Institute of Medicinal and Aromatic Plants (CIMAP)LucknowIndia
  2. 2.Department of BotanyHemwati Nandan Bahuguna Garhwal University (Pauri Campus)Garhwal-246174India
  3. 3.Department of BotanyShia Post Graduate CollegeLucknow-226020India

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