Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers


Chlorophytum borivilianum is a traditional medicinal plant distributed throughout the tropics and subtropics. In the present investigation, AFLP analysis was used to assess the genetic similarity among 34 accessions. Nine primer sets of AFLP amplified 612 fragments, of which 246 fragments were found to be polymorphic. The average number of polymorphic bands per AFLP primer pair was 27.33. The amplified fragments ranged from 50 base pairs to 600 base pairs. Significant correlation was observed between total number of amplified fragments and polymorphic bands (p > 0.05) per primers. Cluster analysis based on AFLP data revealed limited genetic variation within the thirty four accessions collected from various parts of Central Indian forests.

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Fig. 1
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Fig. 3



Simple sequence repeat


Amplified fragment length polymorphism


Random amplified polymorphic DNA


Inter simple sequence repeats


Restriction fragment length polymorphism


Polymorphic information content


Deoxyribonucleic acid


Polymerase chain reaction


Unweighted pair group method with the arithmetic averaging algorithm


Principal component analysis


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We are thankful for grants from Madhya Pradesh Minor Forest Produce Cooperative Ltd., Bhopal, MP, India and Department of Plant Physiology, Jawaharlal Nehru Agricultural University, Jabalpur, India for providing accessions of C. borivilianum.

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Correspondence to Sharad Tiwari.

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Tripathi, N., Saini, N., Nair, P. et al. Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers. Physiol Mol Biol Plants 18, 161–167 (2012).

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  • Accessions
  • Genetic variation
  • Polymorphic
  • Primer