Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers

Abstract

Chlorophytum borivilianum is a traditional medicinal plant distributed throughout the tropics and subtropics. In the present investigation, AFLP analysis was used to assess the genetic similarity among 34 accessions. Nine primer sets of AFLP amplified 612 fragments, of which 246 fragments were found to be polymorphic. The average number of polymorphic bands per AFLP primer pair was 27.33. The amplified fragments ranged from 50 base pairs to 600 base pairs. Significant correlation was observed between total number of amplified fragments and polymorphic bands (p > 0.05) per primers. Cluster analysis based on AFLP data revealed limited genetic variation within the thirty four accessions collected from various parts of Central Indian forests.

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Abbreviations

SSR:

Simple sequence repeat

AFLP:

Amplified fragment length polymorphism

RAPD:

Random amplified polymorphic DNA

ISSR:

Inter simple sequence repeats

RFLP:

Restriction fragment length polymorphism

PIC:

Polymorphic information content

DNA:

Deoxyribonucleic acid

PCR:

Polymerase chain reaction

UPGMA:

Unweighted pair group method with the arithmetic averaging algorithm

PCA:

Principal component analysis

References

  1. Bradley KF, Rieger MA, Collins GG (1996) Classification of Australian garlic cultivars by DNA fingerprinting. Aust J Exp Agric 36:613–618

    Article  CAS  Google Scholar 

  2. Chen Y, Wang L, Ma Y, Xiao H, Tang L, An Q (2010) Genetic diversity of Ligusticum chuanxiong based on inter simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) analyses. Afr J Biotechnol 9:8290–8295

    CAS  Google Scholar 

  3. Doyle LJ, Doyle JJ (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–14

    Google Scholar 

  4. Dwivedi R, Sharma DK (2011) Assessment of genetic diversity among collected genotypes of Chlorophytum borivilianum using random amplified polymorphic DNA (RAPD) markers. Afr J Biotechnol 10:5268–5272

    CAS  Google Scholar 

  5. Geetha K, Maiti S (2002) Biodiversity in Chlorophytum borivilianum Santapau and Fernandes. PGR Newsletter 129:52–53

    Google Scholar 

  6. Gepts P (2004) Crop domestication as a long-term selection experiment. In: Janick J (ed) Plant Breed Rev 24:1–44

  7. Hosokawa K, Minami M, Kawahara K, Nakamura I, Shibata T (2000) Discrimination among three species of medicinal Scutellaria plants using RAPD markers. Planta Med 66:270–272

    PubMed  Article  CAS  Google Scholar 

  8. Ipek M, Ipek A, Simon P (2003) Comparison of AFLPs, RAPD markers, and isozymes for diversity assessment of garlic and detection of putative duplicates in germplasm collections. J Am Soc Hort Sci 128:246–252

    CAS  Google Scholar 

  9. Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

    Google Scholar 

  10. Jatav DS, Tiwari S, Kumar S, Saini N (2011) Study of polymorphism among mother stocks and genetic fidelity of regenerated plants in Chlorophytum spp. using RAPD markers. J Tropic Biodivers 19 (in press).

  11. Katoch M, Kumar R, Pal S, Ahuja A (2010) Identification of Chlorophytum species (C. borivilianum, C. arundinaceum, C. laxum, C. capense and C. comosum) using molecular markers. Indus Crop Prod 32:389–393

    Article  CAS  Google Scholar 

  12. Khan S, Mirza KJ, Abdin MZ (2009) Development of RAPD markers for authentication of medicinal plant Cuscuta reflexa. EurAsia J BioSci 4:1–7

    CAS  Google Scholar 

  13. Kothari SK, Singh K (2001) Evaluation of Safed musli (Chlorophytum borivilianum Santapau and Fernandes) germplasm. J Spices Arom Crops 10:147–149

    Google Scholar 

  14. Koul AK, Gohil RN, Langer A (1997) Prospects of breeding improved garlic in the light of its genetic and breeding systems. Euphytica 28:457–464

    Article  Google Scholar 

  15. Kumar B, Verma AK, Singh HP, Mishra HO, Lal RK (2008) Genetic divergence and correlations study in Chlorophytum borivilianum. J New Seeds 9:321–329

    Google Scholar 

  16. Li W, Li QX, Jian QL, Guang XW (2004) Genetic diversity of Potamogeton maackianus in the Yangtze River. Aquat Bot 80:227–240

    Article  CAS  Google Scholar 

  17. Li WG, Wang BG, Wang JB (2006) Lack of genetic variation of an invasive clonal plant Eichhornia crassipes in China revealed by RAPD and ISSR markers. Aquat Bot 84:176–180

    Article  CAS  Google Scholar 

  18. Maiti S, Geetha KA (2005) Characterization, genetic improvement and cultivation of Chlorophytum borivilianum—an important medicinal plant of India. Pl Genet Res 3:264–272

    Article  Google Scholar 

  19. Mehetre SS, Gomes M, Eapen S (2004) RAPD analysis of hybrid nature of the offspring of Gossypium hirsutum x G. raimondii. Curr Sci 84:24–28

    Google Scholar 

  20. Misra A, Shasany AK, Shukla AK, Darokar MP, Singh SC, Sundaresan V, Singh J, Bagchi GD, Jain SP, Saikia D, Khanuja SP (2010) AFLP markers for identification of Swertia species (Gentianaceae). Genet Mol Res 9:1535–1544

    PubMed  Article  CAS  Google Scholar 

  21. Nayar MP, Shastry ARK (1988) Chlorophytum borivilianum. In: Nayar MP, Sastry ARK (eds) Red data book of Indian plants. Botanical Survey of India, Calcutta, India 2:142

  22. Negi MS, Sabharwal V, Wilson N, Lakshmi kumaran MS (2006) Comparative analysis of the efficiency of SAMPL and AFLP in assessing genetic relationships among Withania somnifera genotypes. Curr Sci 91:464–471

    CAS  Google Scholar 

  23. Ordoñez A, Torres LE, Hidalgo MG, Muñoz JO (2002) Análisis citológico de una variante genetic somática de ajo (Allium sativum L.) tipo rosado. Agriscientia 19:37–43

    Google Scholar 

  24. Oudhia P (2001) Problem perceived by Safed Musli (Chlorophytum borivilianum) grower of Chhattisgarh (India) region: a study. J Med Arom Plant Sci 22(4A)/23 (1A):396–399

    Google Scholar 

  25. Paredes MC, Becerra VV, María I, González A (2008) Low genetic diversity among garlic (Allium sativum L.) accessions detected using random amplified polymorphic DNA (RAPD). Chilean J Agric Res 68:3–12

    Google Scholar 

  26. Pejic I, Ajmone-Marsan P, Morgante M, Kozumplick V, Castiglioni P, Taramino G, Motto M (1998) Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs and AFLPs. Theor Appl Genet 97:1248–1255

    Article  CAS  Google Scholar 

  27. Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Mol Breed 2:225–238

    Article  CAS  Google Scholar 

  28. Rohlf FJ (2000) NYSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter publications, Setauket

    Google Scholar 

  29. Russi L, Moretti C, Raggi L, Albertini E, Falistocco E (2009) Identifying commercially relevant Echinacea species by AFLP molecular markers. Genome 52:912–918

    PubMed  Article  CAS  Google Scholar 

  30. Sarwat M, Das S, Srivastava PS (2008) Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal herb. Plant Cell Rep 27:519–528

    PubMed  Article  CAS  Google Scholar 

  31. Thakur GS, Bag M, Sanodiya BS, Debnath M, Zacharia A, Bhadauriya P, Prasad GB, Bisen PS (2009) Chlorophytum borivilianum: a white gold for biopharmaceuticals and neutraceuticals. Curr Pharm Biotechnol 10:650–666

    PubMed  Article  CAS  Google Scholar 

  32. Tripathi N, Saini N, Tiwari S (2011) Assessment of genetic diversity among Aloe vera accessions using amplified fragment length polymorphism. Int J Med Arom Plants 1:115–121

    Google Scholar 

  33. Vijaya NK, Chavan PD (2009) Chlorophytum borivilianum (Safed musli): a review. Phcog Rev 3:154–169

    Google Scholar 

  34. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Paleman J, Kuiper M, Zabeau M (1995) AFLP a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    PubMed  Article  CAS  Google Scholar 

  35. Wuang YY, Zhou J (1995) Blood relationship analysis and breeding procedures comparison between Chinese and American main tobacco cultivars. Chin Tobacco Sin 3:11–22

    Google Scholar 

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Acknowledgements

We are thankful for grants from Madhya Pradesh Minor Forest Produce Cooperative Ltd., Bhopal, MP, India and Department of Plant Physiology, Jawaharlal Nehru Agricultural University, Jabalpur, India for providing accessions of C. borivilianum.

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Correspondence to Sharad Tiwari.

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Tripathi, N., Saini, N., Nair, P. et al. Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in Central India revealed by AFLP markers. Physiol Mol Biol Plants 18, 161–167 (2012). https://doi.org/10.1007/s12298-012-0108-8

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Keywords

  • Accessions
  • Genetic variation
  • Polymorphic
  • Primer