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Isolation and characterization of ripening related pectin methylesterase inhibitor gene from banana fruit

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Abstract

Identification of ethylene-regulated and ripening-related genes from banana (Musa acuminata Var. Harichaal) fruits using DDRT-PCR led to the isolation of differentially expressed partial cDNA of pectin methylesterase inhibitor (MaPMEI) gene. Its full-length cDNA sequence consisted of a 567 bp ORF, encoding a protein of 189 aa with deduced molecular mass 19.6 kDa. Expression pattern of MaPMEI gene revealed that upon ethylene treatment, this gene is up-regulated initially giving maximum expression in post-climacteric stage then decreases slightly in later stages of ripening. 1-MCP, a known ethylene perception inhibitor, inhibits both fruit ripening as well as the transcript level of this gene. Also, the transcripts of MaPMEI gene were not detected during the short time ethylene treatment suggesting this gene appears to be not directly induced by ethylene. Interestingly, MaPMEI gene showed fruit specific expression that indicates its possible role in the regulations of PMEs in fruits. In silico analysis revealed a predicted signal peptide sequence necessary for localization of MaPMEI in the cell wall. Furthermore, the four Cys residues involved in disulfide bridges are conserved in MaPMEI similar to other PMEIs and invertase inhibitors. Phylogenetic analysis further suggests that the MaPMEI identified in this study is more closely related to PMEIs than to invertase inhibitors.

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References

  • Asif MH, Dhawan P, Nath P (2000) A simple procedure for the isolation of high quality RNA from ripening banana fruit. Plant Mol Biol Rep 18:109–115

    Article  CAS  Google Scholar 

  • Balestrieri C, Castaldo D, Giovane A, Quagliuolo L, Servillo L (1990) A glycoprotein inhibitor of pectin methylesterase in kiwi fruit. Eur J Biochem 193:183–187

    Article  PubMed  CAS  Google Scholar 

  • Camardella L, Carratore V, Ciardiello MA, Servilo L, Balestrieri C, Giovane A (2000) Kiwi protein inhibitor of pectin methylesterase. Eur J Biochem 267:4561–4565

    Article  PubMed  CAS  Google Scholar 

  • Carpita NC, Gibeaut DM (1993) Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J 3:1–30

    Article  PubMed  CAS  Google Scholar 

  • Castaldo D, Loboi A, Quagliuolo L, Servillo L, Balestrieri C, Giovane A (1991) Orange juices and concentrates stabilization by a protein inhibitor of pectin methylesterase. J Food Sci 56:1632–1634

    Article  CAS  Google Scholar 

  • Di Matteo A, Giovane A, Raiola A, Camardella L, Bonivento D, De Lorenzo G, Cervone F, Bellincampi D, Tsernoglou D (2005) Structural basis for the interaction between pectin methyl esterase and a specific inhibitor protein. Plant Cell 17:849–858

    Article  PubMed  Google Scholar 

  • Gaffe J, Tiznado ME, Handa AK (1997) Characterization and functional expression of a ubiquitously expressed tomato pectin methylesterase. Plant Physiol 114:1547–1556

    Article  PubMed  CAS  Google Scholar 

  • Giovane A, Servillo L, Balestrieri C, Quagliuolo L, La Ratta B, Loiudice R, Castaldo D (1994) Purification and characterization of three forms of pectin methylesterase from tomato fruit. J Food Biochem 17:339–341

    Article  CAS  Google Scholar 

  • Giovane A, Servillo L, Balestrieri C, Raiola A, D’Avino R, Tamburrini M, Ciardiello MA, Camardella L (2004) Pectin methylesterase inhibitor. Biochim Biophys Acta 1696:245–252

    PubMed  CAS  Google Scholar 

  • Gupta SM, Srivastava S, Sane AP, Nath P (2006) Differential expression of genes during banana fruit development, ripening and 1-MCP treatment: presence of distinct fruit specific, ethylene induced and repressed expression. Postharvest Biol Technol 42:16–22

    Article  CAS  Google Scholar 

  • Gupta SM, Srivastava S, Gupta S, Ahmed Z (2008) Genetic manipulation of fruit ripening: using Antisense mRNA strategies. J Appl Biosci 34:115–123

    Google Scholar 

  • Hao Y, Huang X, Mei X, Li R, Zhai Z, Yin S, Huang Y, Luo Y (2008) Expression, purification and characterization of pectin methylesterase inhibitor from kiwi fruit in Escherichia coli. Protein Expr Purif 60:221–224

    Article  PubMed  CAS  Google Scholar 

  • Hong MJ, Kim DY, Lee TG, Jeon WB, Seo YW (2010) Functional characterization of pectin methyl esterase inhibitor (PMEI) in wheat. Genes Genet Syst 85:97–106

    Article  PubMed  CAS  Google Scholar 

  • Hothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K (2004) Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins. Plant Cell 16:3437–3447

    Article  PubMed  CAS  Google Scholar 

  • Irifune K, Nishida T, Egawa H, Nagatani A (2004) Pectin methylesterase inhibitor cDNA from kiwi fruit. Plant Cell Rep 23:333–338

    Article  PubMed  CAS  Google Scholar 

  • Jiang CM, Li CP, Chang JC, Chang HM (2002) Characterization of pectinesterase inhibitor in jelly fig (Ficus awkeotsang Makino) achenes. J Agric Food Chem 50:4890–4894

    Article  PubMed  CAS  Google Scholar 

  • Jolie RP, Duvetter T, Houben K, Clynen E, Sila DN, Van Loey AM, Hendrickx ME (2009) Carrot pectin methylesterase and its inhibitor from kiwi fruit: Study of activity, stability and inhibition. Innov Food Sci Emerg Technol 10:601–609

    Article  CAS  Google Scholar 

  • Jolie RP, Duvetter T, Van Loey AM, Hendrickx ME (2010) Pectin methylesterase and its proteinaceous inhibitor: a review. Carbohydr Res 345:2583–2595

    Article  PubMed  CAS  Google Scholar 

  • Liang P, Pardee AB (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967–971

    Article  PubMed  CAS  Google Scholar 

  • Lohani S, Trivedi PK, Nath P (2004) Changes in activities of cell wall hydrolases during ethylene induced ripening in banana: effect of 1-MCP, ABA and IAA. Postharvest Biol Technol 31:119–126

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Srivastava S, Gupta SM (2008) Role of differentially expressed ripening related genes and promoters in banana fruit: identified by mRNA DDRT-PCR. Res Environ Life Sci 1:81–90

    Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimo-ny methods.

  • Vandevenne E, Christiaens S, Buggenhout SV, Jolie RP, González-Vallinas M, Duvetter T, Declerck PJ, Hendrickx ME, Gils A, Loey AV (2011) Advances in understanding pectin methylesterase inhibitor in kiwi fruit: an immunological approach. Planta 2:287–298

    Article  Google Scholar 

  • Wu MC, Tseng KC, Huang TH, Chang HM (2002) Pectinesterase Inhibitor in Rubbery Banana (Musa sapientum L.). J Food Sci 67:1337–1340

    Article  CAS  Google Scholar 

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Acknowledgement

Authors are thankful to Department of Biotechnology (DBT), Government of India for funding the project and Council of Scientific and Industrial Research (CSIR), India for providing the Senior Research Fellowship to SS. Director, National Botanical Research Institute (NBRI) is also acknowledged for providing research facilities to carrying out the present work.

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Authors and Affiliations

  1. Plant Gene Expression Laboratory, National Botanical Research Institute, Lucknow, 226001, India

    Sudhakar Srivastava, Aniruddha P. Sane & Pravendra Nath

  2. Molecular Biology and Genetic Engineering Laboratory, Defence Institute of Bio-Energy Research, DRDO, Goraparao, PO. Arjunpur, Haldwani, 263139, India

    Sanjay Mohan Gupta

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  1. Sudhakar Srivastava
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  2. Sanjay Mohan Gupta
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  3. Aniruddha P. Sane
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  4. Pravendra Nath
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Correspondence to Sanjay Mohan Gupta.

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Srivastava, S., Gupta, S.M., Sane, A.P. et al. Isolation and characterization of ripening related pectin methylesterase inhibitor gene from banana fruit. Physiol Mol Biol Plants 18, 191–195 (2012). https://doi.org/10.1007/s12298-012-0102-1

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  • DOI: https://doi.org/10.1007/s12298-012-0102-1

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