Physiology and Molecular Biology of Plants

, Volume 16, Issue 4, pp 333–341 | Cite as

Gibberellic acid mediated co-ordination of calcium and magnesium ameliorate physiological activities, seed yield and fibre yield of Linum usitatissimum L.—a dual-purpose crop

  • Mohammad Nasir Khan
  • Firoz Mohammad
  • Manzer H. Siddiqui
  • M. Naeem
Research Article
First Online:
  • 85 Downloads

Abstract

Commercial cultivation of linseed for both seed and fibre is not keeping pace with increasing demand for linseed products. Although, different strategies are being adopted to produce a dual-purpose linseed crop with good yield of seed and fibre, little progress has been achieved. The present study was carried out to investigate whether application of gibberellic acid (GA3) along with CaCl2 and/or MgSO4 could ameliorate the seed yield in three linseed genotypes ‘Parvati’, ‘Shekhar’ and ‘Shubhra’ without compromising the fibre production. Before sowing the seeds of linseed genotypes were soaked for 8 h in 10−6 M GA3. Forty days after sowing (DAS), the plants were sprayed with 10−6 M GA3 along with 2 kg Ca/ha (Ca2) and/or 0.5 kg Mg/ha (Mg0.5). Treatments comprised of (1) 10−6 M GA3 + Ca0Mg0 (control, T0); (2) 10−6 M GA3 + Ca2Mg0 (T1); (3) 10−6 M GA3 + Ca0Mg0.5 (T2) and (4) 10−6 M GA3 + Ca2Mg0.5 (T3). Performance of the crop was assessed in terms of growth characteristics, physiological and biochemical parameters at 60 and 75 DAS and yield and quality attributes at harvest. Treatment T3 proved best, it enhanced dry weight per plant by 38.2 and 20.6%, PN by 20.7 and 19.1% and gs by 18.2 and 8.8% at 60 and 75 DAS, respectively and seed yield by 39.6%, oil yield by 46.9% and fibre yield by 36.9% at harvest. Further, a decrease in lodging by 13.9% was recorded. Of the three genotypes tested, all exhibited significant difference for all the parameters studied, except for leaf-N content, biological yield and iodine value which showed no difference. However, ‘Shubhra’ performed better than ‘Parvati’.

Keywords

Linseed Fibre yield Lodging Photosynthesis Carbonic anhydrase 

References

  1. Achard P, Gusti A, Cheminant S, Alioua M, Dhondt S, Coppens F, Beemster GTS, Genschik P (2009) Gibberellin signalling controls cell proliferation rate in Arabidopsis. Curr Biol 19:1188–1193CrossRefPubMedGoogle Scholar
  2. Afroz S, Mohammad F, Hayat S, Siddiqui MH (2005) Exogenous application of gibberellic acid counteracts the ill effect of sodium chloride in mustard. Turk J Biol 29:233–236Google Scholar
  3. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15CrossRefPubMedGoogle Scholar
  4. Arteca RN (1996) Plant growth substances: principles and applications. Chapman and Hall, New YorkGoogle Scholar
  5. Badger MR, Price GD (1994) The role of carbonic anhydrase in photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 45:369–392CrossRefGoogle Scholar
  6. Cakmak I, Kirkby EA (2008) Role of magnesium in carbon partitioning and alleviating photooxidative damage. Physiol Plant 133:692–704CrossRefPubMedGoogle Scholar
  7. Dwivedi RS, Randhawa NS (1974) Evaluation of rapid test for hidden hunger of zinc in plants. Plant Soil 40:445–451CrossRefGoogle Scholar
  8. Easterwood GW (2002) Calcium’s role in plant nutrition. Fluid J 10:16–19Google Scholar
  9. Eastin EF (1978) Total nitrogen determination for plant material containing nitrate. Anal Biochem 85:591–594CrossRefPubMedGoogle Scholar
  10. Fiske CH, Subba Row Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400Google Scholar
  11. Gardner FP, Pearce RB, Mitchell RL (2003) Physiology of crop plants (Reprint). Scientific, JodhpurGoogle Scholar
  12. Hepler PK (2005) Calcium: a central regulator of plant growth and development. Plant Cell 71:2142–2155CrossRefGoogle Scholar
  13. Hesse PR (1971) A text book of soil chemical analysis. John Murray, LondonGoogle Scholar
  14. Hille B (1992) Ionic channels of excitable membranes, 2nd edn. Sinauer Association, Inc, SunderlandGoogle Scholar
  15. Hirschi KD (2004) The calcium conundrum. Both versatile nutrient and specific signal. Plant Physiol 136:2438–2442CrossRefPubMedGoogle Scholar
  16. Hugouvieux V, Murata Y, Young JJ, Kwak JM, Mackesy DZ, Schroeder JI (2002) Localization, ion channel regulation, and genetic interactions during abscisic acid signalling of the nuclear mRNA cap-binding protein, ABH1. Plant Physiol 130:1276–1287CrossRefPubMedGoogle Scholar
  17. Huttly AK, Phillips AL (1995) Gibberellin regulated plant genes. Physiol Plant 95:310–317CrossRefGoogle Scholar
  18. Khan MN (2008) Response of Linum usitatissimum L. to the application of GA3, N, P, Ca and Mg. Ph.D. Thesis. Aligarh Muslim University, Aligarh, IndiaGoogle Scholar
  19. Khan NA, Samiullah (2003) Comparative effect of modes of gibberellic acid application on photosynthetic biomass distribution and productivity of rapeseed-mustard. Physiol Mol Biol Plants 9:141–145Google Scholar
  20. Khan MN, Mohammad F, Manzer HS (2009) Pre-sowing seed treatment and foliar application of gibberellic acid improve seed and fibre yield by inducing net photosynthetic rate and carbonic anhydrase activity of linseed genotypes. Int J Plant Dev Biol 3:34–38Google Scholar
  21. Khan MN, Siddiqui MH, Mohammad F, Naeem M, Khan MMA (2010) Calcium chloride and gibberellic acid protect linseed (Linum usitatissimum L.) from NaCl stress by inducing antioxidative defence system and osmoprotectant accumulation. Acta Physiol Plant 32:121–132CrossRefGoogle Scholar
  22. Lindner RC (1944) Rapid analytical methods for some of the more common inorganic constituents of plant tissues. Plant Physiol 19:76–89CrossRefPubMedGoogle Scholar
  23. Marschner H (2002) Mineral nutrition of higher plants, 2nd edn. Academic, New YorkGoogle Scholar
  24. Mengel K, Kirkby EA (1996) Principles of plant nutrition (Reprint). Panima, New DelhiGoogle Scholar
  25. Mukherji S, Ghosh AK (2005) Plant physiology. New Central Book Agency Pvt. Ltd, KolkataGoogle Scholar
  26. Naeem M, Idrees M, Khan MMA (2009) Calcium ameliorates photosynthetic capacity, nitrate reductase, carbonic anhydrase, nitrogen assimilation, yield and quality of Cassia sophera L.—a medicinal legume. Physiol Mol Biol Plants 15:237–247CrossRefGoogle Scholar
  27. Portis AR (1992) Regulation of ribulose 1, 5-bisphosphate carboxylase oxygenase activity. Annu Rev Plant Physiol Plant Mol Biol 43:415–437CrossRefGoogle Scholar
  28. Samba Murty AVSS, Subrahmanyam NS (1989) A textbook of economic botany. Wiley Eastern, New DelhiGoogle Scholar
  29. Shabala S, Hariadi Y (2005) Effects of magnesium availability on the activity of plasma membrane ion transporters and light-induced responses from broad bean leaf mesophyll. Planta 221:56–65CrossRefPubMedGoogle Scholar
  30. Siddiqui MH, Khan MN, Mohammad F, Khan MMA (2008) Role of nitrogen and gibberellin (GA3) in the regulation of enzyme activities and in osmoprotectant accumulation in Brassica juncea L. under salt stress. J Agron Crop Sci 194:214–224CrossRefGoogle Scholar
  31. Stein WD (1990) Channels, carriers and pumps: an introduction to membrane transport. Academic, New YorkGoogle Scholar
  32. Tiwari HS, Agarwal RM, Bhatt PK (1998) Photosynthesis, stomatal resistance, and related characteristics as influenced by potassium under normal water supply and water stress condition in rice. Indian J Plant Physiol 3:314–316Google Scholar
  33. Verma VS, Raghavaiah CV, Kumar V, Prasad J (2005) Identification of linseed. Linum usitatissimum L. genotypes for high salinity tolerance. J Oilseeds Res 22:75–78Google Scholar
  34. Walker CJ, Weinstein JD (1991) Further characterization of the magnesium chelatase in isolated developing cucumber chloroplasts. Plant Physiol 95:1189–1196CrossRefPubMedGoogle Scholar
  35. Watson DJ (1958) The dependence of net assimilation rate on leaf area index. Ann Bot 22:37–54Google Scholar
  36. Wedding RT, Black MK (1988) Role of magnesium in the binding substrate and effectors to phosphoenolpyruvate carboxylase from a CAM plant. Plant Physiol 87:443–446CrossRefPubMedGoogle Scholar

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2010

Authors and Affiliations

  • Mohammad Nasir Khan
    • 1
  • Firoz Mohammad
    • 1
  • Manzer H. Siddiqui
    • 1
    • 2
  • M. Naeem
    • 1
  1. 1.Plant Physiology Section, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Botany & Microbiology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia

Personalised recommendations