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NAD pattern and NADH oxidase activity in pea (Pisum sativum L.) under cadmium toxicity

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Abstract

Seeds of pea (Pisum sativum L.) were germinated for 5 days by soaking in distilled water or 5 mM cadmium chloride. Compared to the control, cadmium (Cd) caused a reduction in percent germination and embryo growth. Pyridine nucleotide coenzyme concentrations were determined in cotyledons and embryonic axis. Nicotinamide adenine dinucleotide (NADH) oxidase activity was examined. Cd treatment caused a restriction in levels of reduced coenzyme form in the mitochondria and the post-mitochondrial fraction of cotyledons, and embryonic axis. The oxidized coenzyme form has been accumulated by Cd-treated mitochondria of both tissues. It was also found that NADH oxidase activity was stimulated. The relationship between coenzyme levels, seed germination, pea growth, and Cd stress has been reported.

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Acknowledgements

We are thankful to three anonymous reviewers for helpful comments on the manuscript. Financial support for this work was received from the Tunisian Ministry of Higher Education, Scientific Research, and Technology and INRA-Henri Poincaré University (France).

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Authors and Affiliations

  1. Bio-Physiologie Cellulaires, Faculté des Sciences de Bizerte, 7021, Zarzouna, Tunisie

    Moêz Smiri, Abdelilah Chaoui & Ezzedine El Ferjani

  2. Unité Mixte de Recherches, 1136 Interaction arbres-microorganismes INRA-Université Henri-Poincaré, IFR110, Faculté des Sciences, BP 239, 54506, Vandoeuvre cedex, France

    Moêz Smiri, Nicolas Rouhier, Eric Gelhaye & Jean-Pierre Jacquot

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  1. Moêz Smiri
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  2. Abdelilah Chaoui
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  3. Nicolas Rouhier
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  4. Eric Gelhaye
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  5. Jean-Pierre Jacquot
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  6. Ezzedine El Ferjani
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Correspondence to Moêz Smiri.

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Smiri, M., Chaoui, A., Rouhier, N. et al. NAD pattern and NADH oxidase activity in pea (Pisum sativum L.) under cadmium toxicity. Physiol Mol Biol Plants 16, 305–315 (2010). https://doi.org/10.1007/s12298-010-0033-7

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