RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers assay were employed to validate the genetic stability of date palm (Phoenix dactylifera L.) plants multiplied through somatic embryogenesis with upto forty two in vitro subcultures. Out of the 160 RAPD and 21 ISSR primers screened, 30 RAPD and 12 ISSR primers produced a total of 347 (246 RAPDs + 101 ISSRs) clear, distinct and reproducible amplicons, which were monomorphic across all micropropagated plants (27) studied. Thus, a total 8592 bands (number of plants analysed x number of amplicons with all the primers) were generated which exhibited homogeneous banding patterns with both RAPD and ISSR markers. These results indicate that the micropropagation protocol developed by us for rapid in vitro multiplication is appropriate and suitable for clonal propagation of date palm and corroborated the fact that somatic embryogenesis can also be used as one of the safest modes for production of true-to-type plants.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price includes VAT (USA)
Tax calculation will be finalised during checkout.
Ahmed O, Chokri B, Noureddine D, Mohamed M and Mokhtar T (2009). Regeneration and molecular analysis of date palm (Phoenix dactylifera L.) plantlets using RAPD markers. African J.Biotech. 8: 813–820
Andrea B, Maria A and Giovanni V (2004). Genetic stability and uniformity of Foeniculum vulgare Mill. regenerated plants through organogenesis and somatic embryogenesis. Plant sci. 166: 221–227
Bekheet SA, Saker MM, Taha HS and Moursy HA (2001). Plant regeneration via somatic embryogenesis in date palm (Phoenix dactylifera L.). Arab J. Biotechnol. 4: 111–118
Dass HC, Kaul RK, Joshi SP and Bhansali RR (1989). In vitro regeneration of date palm plantlets. Curr. Sci. 58: 22–24
Devarumath R, Nandy S, Rani V, Marimuthu S, Muraleedharan N and Raina S (2002). RAPD, ISSR and RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type) and C. assamica ssp. assamica (Assam-India type). Plant Cell Rep. 21: 166–173
Doyle JJ and Doyle JL (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13–15
Eke CR, Akomeah P and Asemota O (2005). Somatic embryogenesis in date palm (Phoenix dactylifera L.) from apical meristem tissues from ‘zebia’and ‘loko’ landraces. Afr. J. Biotechnol. 4: 244–246
Goto S, Thakur RC and Ishii K (1998). Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers. Plant Cell Rep. 18: 193–197
Hassan S, Bakhsh K, Gill ZA., Maqbool A and Ahmad W (2006). Economics of growing date palm in Punjab, Pakistan. Int. J. Agri. Biol. 8: 788–792
Hirochika H (1993). Activation of tobacco retrotransposons during tissue culture. EMBO J. 12: 2521–2528
Huang WJ., Ning GG, Liu GF and Bao MZ (2009). Determination of genetic stability of long-term micropropagated plantlets of Platanus acerifolia using ISSR markers. Biol. Plant. 53: 159–163
Joshi P and Dhavan V (2007). Assessment of genetic fidelity of micropropagated Swertia chirayita plantlets by ISSR marker assay. Biol. Plant. 51: 22–26.
Lakshman V, Venkataramareddy SR and Neelwarne B (2007). Molecular analysis of genetic stability in long-term micropropagated shoots of banana using RAPD and ISSR markers. Electronic J. of Biotechnol. 10: 1–8
Leroy XJ, Leon K, Charles G and Branchard M (2000). Cauliflower somatic embryogenesis and analysis of regenerant stability by ISSRs. Plant Cell Rep. 19: 1102–1107
Lopes T, Pinto G, Loureiro G, Costa A and Santos C (2006). Determination of genetic stability in cork oak long term somatic embryogenic cultures and derived plantlets using microsatellite markers. Tree Physiol. 26: 1145–1152
Martins M, Sarmento D and Oliveira MM (2004). Genetic stability of micropropagated almond plantlets as assessed by RAPD and ISSR markers. Plant Cell Rep. 23: 492–496
Matthes M, Singh R, Cheah SC and Karp A (2001). Variation in oil palm (Elaeis guineensis Jacq.) tissue culturederived regenerants revealed by AFLPs with methylationsensitive enzymes. Theor. Appl. Genet. 102: 971–979
Murashige T and Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497
Phillips RL, Kaeppler SM and Olhoft P (1994). Genetic instability of plant tissue cultures: Breakdown of normal controls. Proc. Natl. Acad. Sci. USA. 91: 5222–5226
Rahman MH and Rajora OP (2001). Microsatellite DNA somaclonal variation in micropropagated trembling aspen (Populus tremuloides). Plant Cell Rep. 20: 531–536
Reddy MP, Sarla N and Siddiq EA (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica. 128: 9–17
Santos MDM, Buso GCS and Torres AC (2008). Evaluation of genetic variability in micropropagated propagules of ornamental pineapple [Ananas comosus var. bracteatus (Lindley) Coppens and Leal] using RAPD markers. Genet. Mol. Res. 7: 1097–1105
Sharma DR, Dawra S and Chowdhury JB (1984). Somatic embryogenesis and plant regeneration in Date palm (Phoenix dactylifera L.) cv Khadravi through tissue culture. Ind. J. Exp. Biol. 22: 596–598
Sharma SD, Ghosh SA, Mondal BB and Srivastava PS (2004). Somatic embryogenesis in a range of genotypes and genetic stability of the plants derived from somatic embryos using morphological and RAPD markers in Sweet potato. J. Biotech. 6: 119–124
Taha HS, Bakheet SA and EL-Bahr (2003). Alternative approach for micropropagation of the date palm c.v. Zaghlool. Arab J. of Biotechnol. 6: 103–112
Thakur R, Goto S, Ishi K and Jain SM (1999). Monitoring Genetic Stability in Quercus serata Thunb. Somatic Embryogenesis Using RAPD Markers. J. For. Res. 4: 157–160
Tisserat B (1979). Propagation of date palm (Phoenix dactylifera L) in vitro. J. Exp. Bot. 30: 1275–1283
Vasil IK (1987). Developing cell and tissue culture systems for improvement of cereal and grass crops. J. Plant Physiol. 128: 193–218.
Vendrame WA, Kochertm G and Wetzstein HY (1999). AFLP analysis of variation in pecan somatic embryos. Plant Cell Rep. 18: 853–857
Venkatachalam L, Sreedha RV, Bhagyalakshmi N (2007). Micropropagation in banana using high levels of cytokinins does not involve any genetic changes as revealed by RAPD and ISSR markers. Plant Growth Reg. 51: 193–205
About this article
Cite this article
Kumar, N., Modi, A.R., Singh, A.S. et al. Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay. Physiol Mol Biol Plants 16, 207–213 (2010). https://doi.org/10.1007/s12298-010-0023-9
- Date palm
- genetic stability
- molecular marker