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Effect of different plant growth regulators on micro-tuber induction and plant regeneration of Pinellia ternate (Thunb) Briet

Physiology and Molecular Biology of Plants volume 15, Article number: 359 (2009) Cite this article

Abstract

An efficient micropropagation system for Pinellia ternate (Thunb) Briet, a traditional Chinese medicinal plant, has been developed. Petiole and lamina of P. ternate were used as explants and cultured on Murashige and Skoog (MS) medium containing different concentrations of different plant growth regulators. The results indicated that low concentration of 2,4-dicholorophenoxy acetic acid (2,4-D), indole-3-acetic acid (IAA) and α-naphthalene acetic acid (NAA) were suitable for micro-tuber induction, but callus induction rate increased with increasing concentrations of growth regulators. Tubers induction rates of petiole and leaf were (81.8 %–100 %) and (89.4 %–96.0 %) respectively, when 0.2 mg l−1 2, 4-dicholorophenoxy acetic acid, indole-3-acetic acid or α-naphthalene acetic acid were present in the medium. Tubers induction rates of petiole and leaf cultured on MS medium supplemented with 0.2–0.5 mg l−1 6-benzyl amino purine (6-BAP) were (94.1 %–100 %) and (96.0 %–100 %) respectively. When the concentration of 2,4-dicholorophenoxy acetic acid, α-naphthalene acetic acid and 6-benzyl amino purine was increased to 2.0 mg l−1, callus induction rates of petiole and leaf were 100 % and 98.2 %, 91.0 % and 36.0 %, 62.3 % and 70.0 %, respectively. Different concentration of kinetin (KT) and zeatin (ZT) had no significant effect on micro-tuber induction of petiole. Most petioles showed polarity during the cultivation of explants, when supplemented with different concentrations of auxin or cytokinin in the MS medium.

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References

  • Chang L, Xu YM and Xue JP (2007). Formation of microtubers from the petiole of Pinellia ternate (Thunb.) Berit. in vitro and change of endogenous hormones content (Chinese). Journal of Huazhong Agricultural University, 26(5): 612–615.

    CAS  Google Scholar 

  • Chen FQ, Fu Y, Wang DL, Gao X and Wang L (2007). The effect of plant growth regulators and sucrose on the micropropagation and microtuberization of Dioscorea nipponica Makino. Journal of Plant Growth Regulation, 26: 38–45.

    Article  Google Scholar 

  • Chen JH, Cui GY, Liu JY and Tan RX (2003). Pinelloside, an antimicrobial cerebroside from Pinellia ternate. Phytochemistry, 64: 903–906.

    Article  CAS  PubMed  Google Scholar 

  • Dhandapani M, Doo Hwan Kim and Seung Beom Hong (2008). Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus. In vitro Cells Dev. Biol.-Plant, 44: 18–25.

    Article  CAS  Google Scholar 

  • Drugs Institute of Chinese Academy of Medical Sciences (1993). History of Chinese Medicine (book 2) (Chinese). People’s Health Press, Beijing 28–29.

    Google Scholar 

  • Fan MH, Zhou JY, Peng Y and Liu SN (2005). Effect of induction and proliferation of callus of Pinellia ternate (Chinese). Journal of Shandong Chinese Medicine, 24(3): 168–171.

    Google Scholar 

  • Han MH, Yang XW, Zhang M and Zhong GY (2006). Phytochemical study of the rhizome of Pinellia ternate and quantification of phenylpropanoids in commercial Pinellia tuber by RP-LC. Chromatographia, 64: 647–653.

    Article  CAS  Google Scholar 

  • Lin J, Yao JH, Zhou XW, Sun XF and Tang KX (2003). Expression and purification of a novel mannose-binding lectin from Pinellia ternate. Molecular Biotechnology, 25: 215–221.

    Article  CAS  PubMed  Google Scholar 

  • Jarvis BC, Shannon PRM and Yasmin S (1983). Influence IBA and cordyeepin on rooting and RNA synthesis in stem cutting of Phaseolus arureus Roxb. Plant Cell Physiol., 24: 139–142.

    CAS  Google Scholar 

  • Lee YI and Lee N (2003). Plant regeneration from protocorm-derived callus of Cypripedium formosanum. In Vitro Cell. Dev. Biol.-Plant, 39: 475–479.

    Article  Google Scholar 

  • Li XL and Wang XM (2008). Study on the rapid-propagation technical system with the petioles of Pinellia ternate Breit as explants. Journal of Anhui Agricultural Sciences, 36(6): 2247–2248.

    Google Scholar 

  • Luo CK and Peng ZS (2005). Induction of pultaceous callus in Pinellia ternate (Chinese). Journal of China West Normal University (Natural Science), 26(1): 37–43.

    CAS  Google Scholar 

  • Luo GM, Liu XW, Yao ZS, Lai XW and Ge F (2000). Tissue culture and regeneration of Pinellia ternate (Chinese). Journal of Jiangxi College of Traditional Chinese Medicine, 12(3): 125–126.

    Google Scholar 

  • Makunga NP and Staden JV (2008). An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L. Plant Cell Tiss Organ Cult., 92: 63–72.

    Article  Google Scholar 

  • Murugesan D, Doo HK and Seung-Beom H (2008). Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus. In Vitro Cell. Dev. Biol.-Plant, 44: 18–25.

    Google Scholar 

  • Mao ZC and Peng ZS (2003). Progress on research of rapid propagation system of Pinellia ternate (Chinese). China Journal of Chinese Materia Medica, 28(3): 193–195.

    PubMed  Google Scholar 

  • Murashige T and Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant, 15: 473–497.

    Article  CAS  Google Scholar 

  • Peng ZS, Luo CK, Cai P, Mao ZC, Kang CY and Yang J (2007). Rapid propagation of the medicinal plant Pinellia ternate by in vitro leaves culture. Bulgarian Journal of Agricultural Science, 13: 1–6.

    Google Scholar 

  • Ren JH, Chen KR and Xu QF (1983). Organ induction of trefoil Pinellia ternate (Chinese). Plant Physiology Communications, (4): 44–45.

  • Shoyama Y, Hatano K and Nishioka I (1983). Clonal Multiplication of Pinellia ternate by Tissue Culture. Planta Med., 49(1): 14–l6.

    Article  CAS  PubMed  Google Scholar 

  • Wang JL, Liao XR, Zhang HM, Du JF and Chen PL (2003). Accumulation of chlorogenic acid in cell suspension cultures of Eucommia ulmoides Oliv. Plant Cell Tiss Organ Cult., 74(2): 193–195.

    Article  CAS  Google Scholar 

  • Xu XM, Lu DX, Sun M, Zhang M and Chen M (2005). Clustered shoot induction and rapid propagation on Pinellia ternate (Chinese). Journal of Chinese Medicinal Materials, 28(12): 1052–1053.

    PubMed  Google Scholar 

  • Xue JP, Zhang AM, Ge HL and Sheng W (2004). Technique on artificial seeds of Pinellia ternate (Chinese). China Journal of Chinese Materia Medica, 29(5): 402–405.

    PubMed  Google Scholar 

  • Yu Y and Wei ZM (2008). Increased oriental armyworm and aphid resistance in transgenic wheat stably expressing Bacillus thuringiensis (Bt) endotoxin and Pinellia ternate agglutinin (PTA). Plant Cell Tiss Organ Cult., 94: 33–44.

    Article  CAS  Google Scholar 

  • Zeng LB, Zhou JY, Zhao J and Xiong L (1995). Effects of different plant growth regulator on morphogenetic in tissue culture of Pinellia ternate (Chinese). Journal of Anhui Normal University, 18(3): 42–46.

    Google Scholar 

  • Zhang AM, Yang SY, Xue JP, Sheng W, Gao X and Zhu YF (2005). Effects of different factors on direct induction of microtubers from explants in Pinellia ternate (Chinese). China Journal of Chinese Materia Medica, 30(8): 576–579.

    PubMed  Google Scholar 

  • Zhang XW, Wang XF and Zhang XC (2006). Research advances on Pinellia ternate (Chinese). Research and Practice on Chinese Medicines, 20(6): 57–61.

    Google Scholar 

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Affiliations

  1. College of Life and Environment Sciences, Minzu University of China, Beijing, 100081, People’s Republic of China

    Junli Wang, Qian Wang, Jue Wang, Yuan Lu, Xuan Xiao & Weizhen Gong

  2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming, 650204, People’s Republic of China

    Jikai Liu

  3. College of Life and Environment Sciences, Minzu University of China, Zhong Guan Cun South Street 27, Beijing, 100081, People’s Republic of China

    Junli Wang

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  1. Junli Wang
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  2. Qian Wang
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  3. Jue Wang
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  4. Yuan Lu
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  5. Xuan Xiao
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  6. Weizhen Gong
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  7. Jikai Liu
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Correspondence to Junli Wang.

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Wang, J., Wang, Q., Wang, J. et al. Effect of different plant growth regulators on micro-tuber induction and plant regeneration of Pinellia ternate (Thunb) Briet. Physiol Mol Biol Plants 15, 359 (2009). https://doi.org/10.1007/s12298-009-0040-8

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  • DOI: https://doi.org/10.1007/s12298-009-0040-8

Keywords

  • Pinellia ternate (Thunb) Briet
  • Plant growth regulator
  • Micro-tuber
  • Regeneration