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C677T MTHFR Gene Polymorphism is Contributing Factor in Development of Renal Impairment in Young Hypertensive Patients

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Abstract

Homocysteine concentration affected by the activities of the enzymes methylene tetra-hyrdofolate reductase (MTHFR). Polymorphisms in MTHFR gene associated with an impairment of MTHFR activity. Hyperhomocysteinemia is a result of single nucleotide polymorphisms (SNPs) in MTHFR 677 C>T that can cause homocysteine levels in the blood to increase. The purpose of this study is to investigate the relationships between MTHFR C677T (rs1801133) gene polymorphism, changes in homocysteine concentrations and progress of renal impairment in young adult hypertensive patients. Two hundred young hypertensive patients (age 21–24 years) were involved in this study; they were classified into patients with and without renal impairment in addition to 200 age and sex matched healthy controls. All participants were submitted to laboratory investigations as assay of MTHFR gene polymorphism C677T (rs1801133) by PCR/RFLP, determination of lipid profile, homocysteine and folic acid concentrations in addition to urinary albumin creatinine ratio (UACR). The levels of both homocysteine and UACR in the TT genotype patients were higher than those in the CC genotype group. Individuals who carry the T allele were more risky to hypertension and progress to early renal impairment in young age compared with those carrying the C allele [OR 2.02 (1.33–3.08), P < 0.001]. Genetic variants of C677T MTHFR gene and hyperhomocysteinemia may be responsible for rapid progress of renal impairment in Egyptian young age hypertensive patients. TT genotype or T allele may be considered as a predisposing factor for both elevated Hcy levels and the development of renal impairment. This study believed that lowering of homocysteine level can reduce renal impairment of hypertensive patients.

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Acknowledgements

The authors wish to thank all participants of this study.

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Authors and Affiliations

  1. Clinical Pathology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Hanaa H. Elsaid & Saffaa M. Elalawi

  2. Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Khaled A. El-Hefnawy

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  1. Hanaa H. Elsaid
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  2. Khaled A. El-Hefnawy
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  3. Saffaa M. Elalawi
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Contributions

All authors share in design of the study, Khaled A Elhefnawy collected the patients, gathered the clinical data. Hanaa H. Elsaid and Saffa M. Elalawi did the laboratory work of the study. All authors were involved in drafting the article or revising it critically for important intellectual content, and all authors approved the final version to be published.

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Correspondence to Hanaa H. Elsaid.

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The ethical approval was taken from the Zagazig University Ethics Committee guidelines in Egypt according the Declaration of Helsinki.

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Elsaid, H.H., El-Hefnawy, K.A. & Elalawi, S.M. C677T MTHFR Gene Polymorphism is Contributing Factor in Development of Renal Impairment in Young Hypertensive Patients. Ind J Clin Biochem 36, 213–220 (2021). https://doi.org/10.1007/s12291-020-00890-w

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  • DOI: https://doi.org/10.1007/s12291-020-00890-w

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