Abstract
The purpose of this study was to investigate the Bcl2, P53 and apoptosis changes against skin cancer in experimental animals. Skin cancer is the most common form of human cancer. It is estimated that over 1 million new cases occur annually. The annual rates of all forms of skin cancer are increasing each year, representing a growing public concern. It has also been estimated that nearly half of all Americans who live to age 65 are likely to develop skin cancer at least once. Skin cancer was induced in rats by Di Methyl Benz (a) Anthracene at the dosage of DMBA (5 µg) per animal, three times a week for 28 weeks after conformation of skin cancer treated with Paclitaxel and Di allyl sulfide for 30 days. The levels of Bcl2 gene expression were significantly decreased and P53gene expression were markedly increased in Paclitaxel and Di allyl sulfide treated animals when compared with cancer bearing animals. The treatment with combination of Paclitaxel and Di allyl sulfide effectively reduced Bcl2 protein expression and also increased P53gene expression. Moreover, the levels of Bcl2 and P53 a good indicators of restoring the skin architecture, were also reversed in skin damage subjects after treatment with the herbal compounds preparation. So, from the obtained results it is concluded that a combination of Paclitaxel and Di allyl sulfide is capable of restoring the skin architecture and can also increase the apoptosis activities in skin cancer rats.
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References
Khanolkar VR, Suryabhai B. Cancer in relation to usages. Arch Path. 1945;40:351–61.
Ambros-Rudolph CM, Hofmann-Wellenhof R, Richtig E, et al. Malignant melanoma in marathon runners. Arch Dermatol. 2006;142:1471–4.
Sims P, Grover, PL. Polycyclic hydrocarbons and cancer. vol. 3, New York: Academic Press; 1981. pp. 117–81.
Masaaki Miyata, Masayuki Furukawa, Koichi Takahashi, Frank J, Gonzalez and Yasushi Yamazoe. Mechanism of 7, 12 Dimethyl benz (a) anthracene induced munotoxicity. Role of metabolic activation at the target organ. Jpn. J. Pharmacol. 2001;80:x302–9.
Ali M, Thomson M, Afzal M. Garlic and onions: their effect on eicosanoid metabolism and its clinical relevance. Prostaglandins Leukot Essent Fatty Acids. 2000;62:55–73.
Adams JM, Cory S. The Bcl-2 protein family: arbiters of cell survival. Science. 1998;281:1322–6.
Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol. 2008;9:47–59.
Creagh EM, Conroy H, Martin SJ. Caspase-activation pathways in apoptosis and immunity. Immunol Rev. 2003;193:10–21.
Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998;281:1312–6.
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin’s phenol reagent. J Biol Chem. 1951;193:265–76.
Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:156–9.
Laemmli UK. Cleavage of structural protein during the assembly of the Head of Bacteriophage T4. Nature. 1970;227:680–5.
Zar JH. Biostatistical analysis. Englewood Cliffs: Prentice-Hall; 1984.
Jon Geske F, Gerschenson LE. The biology of apoptosis. Hum Pathol. 2001;32(10):1029–38.
Koty PP, Zhang H, Franklin WA, Samuel A, Landreneau R, et al. In vivo expression of p53 and BCL-2 and their role in pro-grammed cell death in premalignant and malignant lung lesions. Lung Cancer. 2002;35:155–63.
Balasenthil S, Rao KS, Nagini S. Garlic induce apoptosis during DMBA induced hamster buccal pouch carcinogenesis. Oral Oncol. 2002;38:431–6.
Pierce GB, Speers WC. Tumors as caricatures of the process of tissue renewal: prospects for therapy by directing differentiation. Cancer Res. 1988;48:1996–2004.
Nancy A. Thornberry and Yuri Lazebnik (1998). Caspases: Enemies within. Science, 281:1312–21.
Eerola A-K, Ruokolainen H, Soini Y, Raunio H, Paakkol P. Accelerated apoptosis and low Bcl2 expression associated with neuroendocrine differentiation predict shortened survival in operated large cell carcinoma of the lung. Pathol Oncol Res. 1999;5(3):179–86.
Chaudhary KS, Abel PD, Lalani EN. Role of Bcl-2 gene family in prostate cancer progression and its implications for therapeutic intervention. Environ. Health. Perspect 1999;107(Suppl 1):49–57.
Sgadari C, Toschi E, Palladino C, Barillari G, Carlei D, Cereseto A, et al. Mechanism of paclitaxel activity in Kaposi’s sarcoma. J Immunol. 2000;165:509–17.
Torres K, Horwitz SB. Mechanisms of Taxol-induced cell death are concentration dependent. Cancer Res. 1998;58(16):3620–6.
Saito M, Korsmeyer SJ, Schlesinger PH. BAX-dependent trans- port of cytochrome C reconstituted in pure liposomes. Nat Cell Biol. 2000;2:553–5.
Lu W, et al. Nuclear exclusion of p53 in a subset of tumors requires MDM2 function. Oncogene. 2000;19:232–40.
Burns PA. Loss of heterozygosity and mutational alterations of the p53 gene in skin tumors of interspecific hybrid mice. Onco-gene. 1991;6:2363–9.
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Muninathan, N. Amelioration of Combination of Paclitaxel and Di Allyl Sulfide on the Alterations of Bcl2, P53 and Apoptosis Changes Against 7,12 Di Methyl Benz (A) Anthracene Induced Skin Cancer in Experimental Animals. Ind J Clin Biochem 36, 143–150 (2021). https://doi.org/10.1007/s12291-019-0817-7
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DOI: https://doi.org/10.1007/s12291-019-0817-7