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Expression of FOXP3 and GATA3 Transcription Factors Among Bronchial Asthmatics in Northern Population

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Abstract

Asthma is a common chronic airways inflammatory disorder in which the expression of genes for the transcription factors FoxP3 and GATA3 plays crucial roles in activation of specific T cells population and pathogenesis of asthma. Recent data have shown that Hb, Eosinophils, total leucocytes count (TLC), absolute eosinophil count (AEC), and IgE, may be involved in adversely influencing the status of several chronic diseases including asthma. In this communication, we have carried out a case control study in order to evaluate the expression of FoxP3, GATA-3 genes in 80 bronchial asthmatic patients using real time polymerase chain reaction technique, and also to analyse and compare the values of Hb, TLC, AEC, and IgE in asthmatics with 80 control subjects. The numbers of eosinophils and total leucocytes and the level of serum IgE were higher in asthmatics compared to healthy subjects. The relative expressions of FoxP3 and GATA-3 genes in control versus asthmatics were 12.42 ± 1.413 versus 5.79 ± 0.260 (P value = < 0.0001) and 4.731 ± 0.350 versus 8.415 ± 0.359 (P value = 0.0043), respectively. The asthmatics displayed comparatively decreased level of FoxP3 expression and higher level of GATA-3 expression. There was a positive and significant correlation between the level of IgE and expression of GATA-3 in asthmatics. Relatively lower level of FoxP3 mRNA expression in bronchial asthmatics may be linked with the sustained inflammatory process and decreased immune tolerance by asthmatics. A positive correlation of GATA-3 expression with the increase in IgE level shows it to be a characteristic of asthma. However, extensive work is required to delineate the targets involved in the pathogenesis of asthma for adequate therapeutic interventions.

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References

  1. National Institutes of Health; National Heart, Lung and Blood Institute Global Initiative for Asthma; Global strategy for asthma management and prevention. Washington, DC: National Institutes of Health; National Heart, Lung and Blood Institute, 2003 Updated 2004. Available at http://www.ginasthma.com. NIH publication No. 02-3659, 2009.

  2. Pandey R, Sharma B. Therapeutic applications of antiasthmatics, consequences and remedies. EC Pharmacol Toxicol. 2018;6(7):580–9.

    Google Scholar 

  3. Kupczyk M. Long-term deterioration of lung function in asthmatic outpatients. Respiration. 2004;71:233–40.

    Article  Google Scholar 

  4. Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM. Asthma From bronchoconstriction to airways inflammation and remodeling. Am J Respir Crit Care Med. 2000;161:1720–45.

    Article  CAS  Google Scholar 

  5. Akdis M, Akdis CA. Therapeutic manipulation of immune tolerance in allergic disease. Nat Rev Drug Discov. 2009;8:645–60.

    Article  CAS  Google Scholar 

  6. Pyzik M, Piccirillo CA. TGF-beta1 modulates FoxP3 expression and regulatory activity in distinct CD41 T cell subsets. J Leukoc Biol. 2007;82:335–46.

    Article  CAS  Google Scholar 

  7. Trzonkowski P, Szmit E, Myœliwska J, Myœliwski A. CD41CD251 T regulatory cells inhibit cytotoxic activity of CTL and NK cells in humans—impact of immunosenescence. Clin Immunol. 2006;119:307–16.

    Article  CAS  Google Scholar 

  8. Seroogy CM, Gern JE. The role of T regulatory cells in asthma. J Allergy Clin Immunol. 2005;116:996–9.

    Article  CAS  Google Scholar 

  9. Klunker S, Chong MM, Mantel PY. Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. J Exp Med. 2009;206:2701–15.

    Article  CAS  Google Scholar 

  10. Moorman JE, Rudd RA, Johnson CA. National surveillance for asthma–United States, 1980–2004. MMWR Surveill Summ. 2007;56:1–54.

    PubMed  Google Scholar 

  11. Gregg R, Smith CM, Clark FJ. The number of human peripheral blood CD4+ CD25high regulatory T cells increases with age. Clin Exp Immunol. 2005;140:540–6.

    Article  CAS  Google Scholar 

  12. Knudson R, Slavin R, Lebowitz M, Burrows B. The maximal expiratory flow-volume curve. Normal standards, variability, and effects of age. Am Rev Respir Dis. 1976;113:587–600.

    CAS  PubMed  Google Scholar 

  13. Broide DH. Immunologic and inflammatory mechanisms that drive asthma progression to remodeling. J Allergy Clin Immunol. 2008;121:560–70.

    Article  CAS  Google Scholar 

  14. Robinson DS. The role of the T cell in asthma. J Allergy Clin Immunol. 2010;126(6):1081–91.

    Article  CAS  Google Scholar 

  15. Martinis M, Benedetto MC, Mengoli LP, Ginaldi L. Senile osteoporosis: is it an immune-mediated disease? Inflamm Res. 2006;55:399–404.

    Article  Google Scholar 

  16. OShea JJ, Paul WE. Mechanisms underlying lineage commitment and plasticity of helper CD4 + T cells. Science. 2010;327:1098–102.

    Article  CAS  Google Scholar 

  17. Zhu J, Yamane H, Paul WE. Differentiation of effector CD4 T cell populations. Annu Rev Immunol. 2010;28:445–89.

    Article  CAS  Google Scholar 

  18. Mantel PY, Kuipers H, Boyman O. GATA3-driven Th2 responses inhibit TGF-beta1-induced FOXP3 expression and the formation of regulatory T cells. PLoS Biol. 2007;5:2847–61.

    Article  CAS  Google Scholar 

  19. Lin Y-L, Shieh C-C, Wang J-Y. The functional insufficiency of human CD41 CD25 high T-regulatory cells in allergic asthma is subjected to TNF-a modulation. Allergy. 2008;63:67–74.

    Article  CAS  Google Scholar 

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Funding

This research was funded by the UPCST-Lucknow, India (Grant No. CST/SERPD/D-290).

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Authors and Affiliations

  1. Departments of Pulmonary and Critical Care Medicine, KGMU, Lucknow, India

    Rashmi Pandey & Ved Prakash

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  1. Rashmi Pandey
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  2. Ved Prakash
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Contributions

RP designed and executed the experiments, analysed the data and wrote the manuscript. VP developed the hypothesis, provided clinical samples, generated facilities and funding acquisition and edited the manuscript.

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Correspondence to Ved Prakash.

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Pandey, R., Prakash, V. Expression of FOXP3 and GATA3 Transcription Factors Among Bronchial Asthmatics in Northern Population. Ind J Clin Biochem 36, 88–93 (2021). https://doi.org/10.1007/s12291-019-00853-w

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  • DOI: https://doi.org/10.1007/s12291-019-00853-w

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