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The Nephroprotective Effect of Lycopene on Renal Ischemic Reperfusion Injury: A Mouse Model

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Abstract

Acute kidney injury (AKI) is characterized by fast decline in renal function within a short period of time. Renal ischemic–reperfusion (I–R) injury is the main cause of AKI. This study aims to investigate the possible nephroprotective effect of lycopene on renal ischemic–reperfusion injury in mice model. Forty Swiss Albino adult male mice were randomly allocated onto one of the four study groups: sham group: mice had median laparotomy under anesthesia with no procedures performed, renal tissues and blood samples were collected. ischemic–reperfusion group (I–R-control): mice underwent median laparotomy under anesthesia, followed by 30 min bilateral renal ischemia. Renal tissues and blood samples were collected after 2 h from reperfusion. Vehicle-treated group: mice were pretreated with intra 1% dimethyl sulfoxide 30 min before inducing ischemia. Lycopene-treated group: mice were pretreated with 10 mg/kg intraperitoneal injection of lycopene 30 min before inducing renal ischemia. Renal tissues, and blood samples were collected after 2 h from reperfusion. Blood and tissue samples were collected to look for evidence of inflammation and necrosis. Blood urea nitrogen, serum creatinine as well as plasma NGAL levels were significantly increased in the active control group (P ≤ 0.05), when compared to the sham group. Similarly, renal levels of Notch2/Hes 1, TLR 2, IL-6, Bax, and F2-isoprostane were significantly increased in the active control group as compared to the sham group (P ≤ 0.05). Moreover, lycopene treatment was found to be significantly effective in reducing the increased levels of these markers after I–R injury (P ≤ 0.05).

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Acknowledgements

We acknowledge the support of technical staff at Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Kufa.

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This work received no external funds.

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Authors and Affiliations

  1. College of Pharmacy, Al-Kafeel University, Al-Najaf, Iraq

    Yasmeen A. Hussien

  2. Faculty of Medicine, University of Kufa, Al-Najaf, Iraq

    Hussien Abdalkadim, Waddah Mahbuba & Najah R. Hadi

  3. School of Life Sciences, College of Science, Health and Engineering, La Trobe University, Bundoora, VIC, 3086, Australia

    Dina A. Jamil & Hayder A. Al-Aubaidy

Authors
  1. Yasmeen A. Hussien
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  2. Hussien Abdalkadim
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  3. Waddah Mahbuba
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  4. Najah R. Hadi
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  5. Dina A. Jamil
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  6. Hayder A. Al-Aubaidy
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Correspondence to Hayder A. Al-Aubaidy.

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Hussien, Y.A., Abdalkadim, H., Mahbuba, W. et al. The Nephroprotective Effect of Lycopene on Renal Ischemic Reperfusion Injury: A Mouse Model. Ind J Clin Biochem 35, 474–481 (2020). https://doi.org/10.1007/s12291-019-00848-7

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  • DOI: https://doi.org/10.1007/s12291-019-00848-7

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