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Psidium guajava Leaf Extracts and Their Quercetin Protect HepG2 Cell Lines Against CCL4 Induced Cytotoxicity

Indian Journal of Clinical Biochemistry volume 34, pages 324–329 (2019)Cite this article

Abstract

The present study was carried out to evaluate the in vitro cytoprotective effects of Psidium guajava and their isolated quercetin fraction to reduce the CCl4 (carbon tetrachloride) induced toxicity in HepG2 cell lines (Hepatocellular carcinoma G2). Silymarin was used as a standard drug to compare the protective effects of plant extracts in infected cell lines. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay, cell viability assay, leakage parameters [Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Lactate dehydrogenase (LDH)], lipid peroxidation and reduced glutathione (GSH) levels were used to find out the protection of human derived HepG2 cells against CCl4-induced damage. The levels of cytotoxicity, viability and GSH were reduced. While the activities of AST, ALT, LDH and lipid peroxidation was increased in CCl4-treated groups. The treatment of P. guajava and their isolated quercetin fractions (100, 200, 300 µg/mL) decreased the elevated levels of all these parameters. The results of the present study suggest that the ethanolic extract of P. guajava leaf and their isolated quercetin fractions can able to reduce the CCl4-induced cytotoxicity in HepG2 cell lines.

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Authors and Affiliations

  1. Department of Biochemistry, M.I.E.T. Arts and Science College, Tiruchirappalli, Tamil Nadu, 620 007, India

    K. Vijayakumar

  2. Department of Animal Science, Centre for Pheromone Technology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

    R. L. Rengarajan

  3. Department of Biotechnology, Yeungnam University, Gyeongsan, Republic of Korea

    R. Radhakrishnan

  4. Centre of Excellence in Genomic Medicine Research, King Abdul Aziz University, P.O. Box 80216, Jeddah, 21589, Kingdom of Saudi Arabia

    Shilu Mathew

  5. King Fahd Medical Research Centre, King Abdul Aziz University, P.O. Box 802160, Jeddah, 21589, Kingdom of Saudi Arabia

    Shilu Mathew & Ishtiaq Qadri

  6. Department of Human Genetics and Molecular Biology, Bharathiar University, Coimbatore, Tamil Nadu, 641 046, India

    A. Vijaya Anand

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  1. K. Vijayakumar
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  2. R. L. Rengarajan
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  3. R. Radhakrishnan
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  4. Shilu Mathew
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Correspondence to A. Vijaya Anand.

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Vijayakumar, K., Rengarajan, R.L., Radhakrishnan, R. et al. Psidium guajava Leaf Extracts and Their Quercetin Protect HepG2 Cell Lines Against CCL4 Induced Cytotoxicity. Ind J Clin Biochem 34, 324–329 (2019). https://doi.org/10.1007/s12291-018-0752-z

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  • DOI: https://doi.org/10.1007/s12291-018-0752-z

Keywords

  • Carbontetrachloride
  • Psidium guajava
  • Silymarin
  • Hepatocellular carcinoma G2
  • Aspartate aminotransferase
  • Alanine aminotransferase and Lactate dehydrogenase