Psidium guajava Leaf Extracts and Their Quercetin Protect HepG2 Cell Lines Against CCL4 Induced Cytotoxicity

  • K. Vijayakumar
  • R. L. Rengarajan
  • R. Radhakrishnan
  • Shilu Mathew
  • Ishtiaq Qadri
  • A. Vijaya Anand
Original Research Article


The present study was carried out to evaluate the in vitro cytoprotective effects of Psidium guajava and their isolated quercetin fraction to reduce the CCl4 (carbon tetrachloride) induced toxicity in HepG2 cell lines (Hepatocellular carcinoma G2). Silymarin was used as a standard drug to compare the protective effects of plant extracts in infected cell lines. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay, cell viability assay, leakage parameters [Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Lactate dehydrogenase (LDH)], lipid peroxidation and reduced glutathione (GSH) levels were used to find out the protection of human derived HepG2 cells against CCl4-induced damage. The levels of cytotoxicity, viability and GSH were reduced. While the activities of AST, ALT, LDH and lipid peroxidation was increased in CCl4-treated groups. The treatment of P. guajava and their isolated quercetin fractions (100, 200, 300 µg/mL) decreased the elevated levels of all these parameters. The results of the present study suggest that the ethanolic extract of P. guajava leaf and their isolated quercetin fractions can able to reduce the CCl4-induced cytotoxicity in HepG2 cell lines.


Carbontetrachloride Psidium guajava Silymarin Hepatocellular carcinoma G2 Aspartate aminotransferase Alanine aminotransferase and Lactate dehydrogenase 


Compliance with Ethical Standards

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Association of Clinical Biochemists of India 2018

Authors and Affiliations

  • K. Vijayakumar
    • 1
  • R. L. Rengarajan
    • 2
  • R. Radhakrishnan
    • 3
  • Shilu Mathew
    • 4
    • 5
  • Ishtiaq Qadri
    • 5
  • A. Vijaya Anand
    • 6
  1. 1.Department of BiochemistryM.I.E.T. Arts and Science CollegeTiruchirappalliIndia
  2. 2.Department of Animal Science, Centre for Pheromone TechnologyBharathidasan UniversityTiruchirappalliIndia
  3. 3.Department of BiotechnologyYeungnam UniversityGyeongsanRepublic of Korea
  4. 4.Centre of Excellence in Genomic Medicine ResearchKing Abdul Aziz UniversityJeddahKingdom of Saudi Arabia
  5. 5.King Fahd Medical Research CentreKing Abdul Aziz UniversityJeddahKingdom of Saudi Arabia
  6. 6.Department of Human Genetics and Molecular BiologyBharathiar UniversityCoimbatoreIndia

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