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Chronomics of Circulating Plasma Lipid Peroxides and Antioxidant Enzymes in Renal Stone Formers

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Abstract

The chronome of lipid peroxidation and anti-oxidant defense mechanisms may relate to the efficacy and management of time qualified preventive therapeutic and dietary interventions. One hundred renal stone patients, 20–60 years of age, and 50 clinically healthy volunteers, 21–45 years, were synchronized for 1 week with diurnal activity from 06:00 to 22:00 and nocturnal rest. All subjects took their usual meals three times daily (breakfast around 08:30, lunch around 13:00, and dinner around 20:30) with usual fluid intake. Drugs known to affect free radical system were not taken. Blood samples were collected at 6-h intervals for 24-h under standardized, presumably 24-h synchronized conditions. Determinations included plasma lipid peroxides, in terms of malondialdehyde (MDA) and blood superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) activities. A marked circadian variation was demonstrated for each studied variable by population-mean cosinor in renal stone patients and healthy participants (p < 0.001). By comparison to healthy subjects, parameter tests indicate that the stone formers had a higher MESOR of MDA, but a lower MESOR of SOD, GPx, GR and CAT. Furthermore, the patients also differed from the healthy controls in terms of their circadian amplitude and acrophase (tested jointly) of all variables (p < 0.001). Mapping the broader time structure with multifrequency circadian characteristics of oxidants and anti-oxidants is needed for exploring their role as marker in the treatment and management of urolithiasis.

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Acknowledgements

The authors are grateful to Hon. Chairman, Shri Guru Ram Rai Education Mission for his constant support and guidance in pursuing such studies in our laboratory. We are also indebted to the staff of the Department of Biochemistry, SGRR Institute of Medical and Health Sciences for their technical assistance. Our special thanks to Dr Rachit Garg for patient enrolment.

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Authors and Affiliations

  1. Department of Biochemistry, NIMS Medical College and Hospital, Shobha Nagar, Jaipur, Rajasthan, 303121, India

    Rajeev Singh Kushwaha & R. C. Gupta

  2. Department of Biochemistry, SGRR Institute of Medical and Health Sciences, Dehradun, 248001, India

    Rajeev Singh Kushwaha, Sumita Sharma & Tariq Masood

  3. Department of Surgery, SGRR Institute of Medical and Health Sciences and Shri Mahant Indiresh Hospital, Dehradun, UK, 248001, India

    J. P. Sharma

  4. Department of Biochemistry, TS Misra Medical College and Hospital, Amausi, Lucknow, UP, 226008, India

    Rajesh K. Singh & Raj K. Singh

  5. Halberg Chronobiology Center, University of Minnesota, Minneapolis, MN, 55455, USA

    Cathy L. Gierke & Germaine Cornelissen

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  1. Rajeev Singh Kushwaha
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  2. R. C. Gupta
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  3. Sumita Sharma
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  5. J. P. Sharma
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Correspondence to Raj K. Singh.

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Kushwaha, R.S., Gupta, R.C., Sharma, S. et al. Chronomics of Circulating Plasma Lipid Peroxides and Antioxidant Enzymes in Renal Stone Formers. Ind J Clin Biochem 34, 195–200 (2019). https://doi.org/10.1007/s12291-017-0726-6

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  • DOI: https://doi.org/10.1007/s12291-017-0726-6

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