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Monotonic Dose Effect of Bisphenol-A, an Estrogenic Endocrine Disruptor, on Estrogen Synthesis in Female Sprague-Dawley Rats

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Abstract

Bisphenol-A (BPA) is a ubiquitous environmental chemical that produces adverse effect on reproduction system due to its potent estrogenic endocrine disruptive activity. The present study was aimed to investigate the monotonic dose effect of BPA on estrogen synthesis in female Sprague-Dawley rats. For this purpose, we administered three different doses of BPA (10, 50, 100 µg/kg bw/day) into rats and analyzed various biochemical, hormonal, molecular and histological parameters. 10 µg BPA treated rats showed significantly decreased levels of phase I detoxification agents (CYP450, Cyt-b5). Overexpression of eNOS with decreased expression of StAR and steroidogenic enzymes (CYP11A1, aromatase) indicate decreased production of estrogen. Increased levels of serum gonadotropins (FSH, LH) and decreased levels of estradiol suggest mimetic action of BPA and its feedback inhibition. Increased body weight, lipid profile status of 10 µg BPA treated rats and histological analysis of ovary and mammary tissue support the study. Overall, our results suggest that BPA exerts its estrogen mimetic effects in a monotonic manner.

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Abbreviations

BPA:

Bisphenol-A

TC:

Total cholesterol

TG:

Triglycerides

HDL-C:

High density lipoprotein-cholesterol

TBARS:

Thiobarbituric acid substances

GSH:

Reduced glutathione

StAR:

Steroidogenic acute regulatory protein

eNOS:

Endothelial nitric oxide synthase

CYP11A1:

Cytochrome P450 monooxygenase

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Acknowledgements

The authors grateful to Prof. J. Thanka, Head, Department of Pathology, Sri Ramachandra Medical College and RI, Sri Ramachandra University, Porur, Chennai for their help in histopathological studies. We gratefully acknowledge the financial assistance from University Grants Commission (UGC), New Delhi, India in the form of Major Research Project.

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Authors and Affiliations

  1. Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Chidambaram, Tamil Nadu, 608 002, India

    Subbaiyan Thilagavathi, Pachaiappan Pugalendhi & Thangarasu Rajakumar

  2. Department of Zoology (DDE), Annamalai University, Annamalainagar, Chidambaram, Tamil Nadu, 608 002, India

    Krishnamoorthy Vasudevan

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  1. Subbaiyan Thilagavathi
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Correspondence to Pachaiappan Pugalendhi.

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Thilagavathi, S., Pugalendhi, P., Rajakumar, T. et al. Monotonic Dose Effect of Bisphenol-A, an Estrogenic Endocrine Disruptor, on Estrogen Synthesis in Female Sprague-Dawley Rats. Ind J Clin Biochem 33, 387–396 (2018). https://doi.org/10.1007/s12291-017-0696-8

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