Protective Effect of Allyl Isothiocyanate on Glycoprotein Components in 7,12-dimethylbenz(a)anthracene Induced Mammary Carcinoma in Rats
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The present study aimed to investigate the protective effect of allyl isothiocyanate (AITC) on glycoprotein components in 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Sprague–Dawley rats. Mammary tumor was induced by a single dose of DMBA (25 mg/rat) injected subcutaneously near mammary gland. The levels of glycoprotein components such as hexose, hexosamine and sialic acid were analyzed colorimetrically in plasma, mammary and liver tissues. We observed an increased levels of glycoprotein components in plasma, mammary and liver tissues in cancer bearing rats. It was further confirmed by Periodic Acid Schiff staining in mammary and liver tissues. Upon oral administration of AITC to DMBA injected rats, the abnormal changes were reverted back to near normal levels and biochemical findings are supported by histological analysis. This could be due to the anti-neoplastic potential of AITC against DMBA-induced mammary carcinogenesis. The result shows that AITC has the potential to inhibit abnormal glycosylation that favors neoplastic transformation.
KeywordsAllyl isothiocyanate 7,12-dimethylbenz(a)anthracene Glycoprotein components Mammary carcinogenesis
We gratefully acknowledged the financial assistance from Indian Council of Medical Research (ICMR), in the form of Senior Research Fellowship (SRF), New Delhi, India to the first author Mr. T. Rajakumar.
This study was funded by Indian Council of Medical Research (ICMR), Government of India (Grant No. 3/2/2/189/2013/NCD-III (OPA-27129)).
Compliance with Ethical Standards
Conflict of interest
The first author (Mr. T. Rajakumar) has received Senior Research Fellowship (SRF), from Indian Council of Medical Research (ICMR), Government of India. The corresponding author (Dr. P. Pugalendhi) and last author (Mrs. S. Thilagavathi) declares that there are no conflicts of interest.
This study was approved by the Institutional Animal Ethics Committee (IAEC), regulated by the Committee for the Purpose of Control and Supervision of Experimental Animals (CPCSEA) (Reg. No. 160/1999/CPCSEA and Proposal No. 983).
This article does not contain any studies with human participants.
- 5.Murray RK. Glycoproteins. Harper’s biochemistry. 24th ed. Appleton and Lange. USA 1996.Google Scholar
- 24.Purushothaman A, Nandhakumar E, Sachdanandam P. Anticancer effect of shemamruthaa (a phytochemical formulation) on 7, 12-Dimethylbenz(a)anthracene induced mammary carcinoma in rats. Asian J Pharm Clin Res. 2012;5:101–7.Google Scholar
- 26.Jagadeesan AJ, Langeswaran K. Gowtham kumar S, Revathy R, Balasubramanian MP. Chemopreventive potential of diosgenin on modulating glycoproteins, TCA cycle enzymes, carbohydrate metabolising enzymes and biotransformation enzymes against N-methyl-N-nitrosourea induced mammary carcinogenesis. Int. J Pharm Pharm Sci. 2013;5:575–82.Google Scholar
- 27.Nandakumar N, Jayaprakash R, Balasubramanian MP. Influence of hesperidin on renal cell surface glycoprotein content, nucleic acids, lysosomal enzymes and macromolecules against 7, 12-dimethylbenz [a] anthracene induced experimental breast carcinoma. J Exp Ther Oncol. 2012;9:265–80.PubMedGoogle Scholar
- 31.Gowtham Kumar S, Ramakrishnan V, Madhusudhanan N, Balasubramanian MP. Antioxidant activity of Allyl isothiocyanate [AITC] against N-nitrosodiethylamine induced experimental liver carcinogenesis. J Pharm Res. 2011;4:3690–4.Google Scholar