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Characterization and Classification of Mesenchymal Stem Cells in Several Species Using Surface Markers for Cell Therapy Purposes

Indian Journal of Clinical Biochemistry volume 33, pages 46–52 (2018)Cite this article

Abstract

Mesenchymal stem cells are multipotent cells capable of replicating as undifferentiated cells, and have the potential of differentiating into mesenchymal tissue lineages such as osteocytes, adipocytes and chondrocytes. Such lineages can then be used in cell therapy. The aim of present study was to characterize bone marrow derived mesenchymal stem cells in four different species, including: sheep, goat, human and mouse. Human bone-marrow mesenchymal stem cells were purchased, those of sheep and goat were isolated from fetal bone marrow, and those of mouse were collected by washing bone cavity of femur and tibia with DMEM/F12. Using flow-cytometry, they were characterized by CD surface antigens. Furthermore, cells of third passage were examined for their osteogenic and adipogenic differentiation potential by oil red and alizarin red staining respectively. According to the results, CD markers studied in the four groups of mesenchymal stem cells showed a different expression. Goat and sheep expressed CD44 and CD166, and weakly expressed CD34, CD45, CD105 and CD90. Similarly, human and mouse mesenchymal cells expressed CD44, CD166, CD105 and CD90 whereas the expression of CD34 and CD45 was negative. In conclusion, although all mesenchymal stem cells display plastic adherence and tri-lineage differentiation, not all express the same panel of surface antigens described for human mesenchymal stem cells. Additional panel of CD markers are necessary to characterize regenerative potential and possible application of these stem cells in regenerative medicine and implantology.

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Abbreviations

MSCs:

Mesenchymal stem cells

CD:

Cluster of differentiation

ESCs:

Embryonic stem cells

iPSCs:

Induced pluripotent stem cells

BM-MSCs:

Bone marrow mesenchymal stem cells

DPBS:

Dulbecco’s phosphate buffered saline

FBS:

Fetal bovine serum

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Author information

Affiliations

  1. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Hori Ghaneialvar & Abbas Sahebghadam Lotfi

  2. Department of Animal Sciences, Faculty of Agriculture, Razi University, Kermanshah, Iran

    Leila Soltani

  3. Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran

    Hamid Reza Rahmani

  4. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Masoud Soleimani

Authors
  1. Hori Ghaneialvar
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  2. Leila Soltani
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  3. Hamid Reza Rahmani
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  4. Abbas Sahebghadam Lotfi
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  5. Masoud Soleimani
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Correspondence to Abbas Sahebghadam Lotfi.

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Ghaneialvar, H., Soltani, L., Rahmani, H.R. et al. Characterization and Classification of Mesenchymal Stem Cells in Several Species Using Surface Markers for Cell Therapy Purposes. Ind J Clin Biochem 33, 46–52 (2018). https://doi.org/10.1007/s12291-017-0641-x

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  • DOI: https://doi.org/10.1007/s12291-017-0641-x

Keywords

  • Mesenchymal stem cells
  • Surface marker
  • Multi potential differentiation