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Association of Mbo I-RFLP at the Renin Locus (rs2368564) with Essential Hypertension

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Abstract

Several lines of experimental and clinical evidence have alluded a pivotal role of renin in blood pressure homeostasis and therefore a relevance of molecular variants of the renin gene and essential hypertension have been speculated. This study was designed to evaluate the pattern, alliance and risk of renin Mbo I (10631A>G; rs2368564) polymorphism at the locus intron 9 for a possible role in modulating essential hypertension in adult population from Gujarat (India). A total of 257 consecutively enrolled essential hypertensive patients and 270 controls were genotyped using polymerase chain reaction-restriction fragment length polymorphism method for the selected marker. Suitable descriptive statistics was used for different variables. Genotypic (x2 10.43, p 0.0054) and allelic (x2 11.46, p 0.0007) distribution of this SNP displayed significant differences between cases and controls with an increased frequency of the A allele (x2 6.275; p 0.0122) and A/A geno-type (x2 8.247; p 0.0041) in hypertensive individuals. However, it showed no deviation from Hardy–Weinberg equilibrium in either affected or control group. A significant association was found in the A/A variant of rs2368564 with essential hypertension (p 0.0032), along with a statistically significant increase in odds of hypertension (OR 1.69; CI 1.46–2.28; p 0.02), even after confounding factors were adjusted in multiple logistic regression analysis and is substantiated by inter-genotypic variations in the mean systolic and diastolic blood pressure in patients. In conclusion, renin 10631A>G gene mutation at the ninth intron play critical roles in BP (dys)regulation and can be implicated in an individual’s susceptibility for hypertension.

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Authors and Affiliations

  1. GMERS Medical College, Himmatnagar, Gujarat, India

    Deepak N. Parchwani

  2. Gujarat Adani Institute of Medical Sciences, Bhuj, Gujarat, India

    Digisha D. Patel

  3. SN Medical College, Jodhpur, Rajasthan, India

    Jairam Rawtani

  4. GCS Medical College, Ahmedabad, Gujarat, India

    Nirupama Dikshit

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  1. Deepak N. Parchwani
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  2. Digisha D. Patel
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  3. Jairam Rawtani
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  4. Nirupama Dikshit
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Correspondence to Deepak N. Parchwani.

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Parchwani, D.N., Patel, D.D., Rawtani, J. et al. Association of Mbo I-RFLP at the Renin Locus (rs2368564) with Essential Hypertension. Ind J Clin Biochem 31, 431–438 (2016). https://doi.org/10.1007/s12291-015-0546-5

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  • DOI: https://doi.org/10.1007/s12291-015-0546-5

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