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Differential Expressions of p53, p53R2, hRRM2 and PBR in Chronic Lymphocytic Leukemia: A Correlation with Intracellular Cholesterol

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Abstract

Regulation of intracellular cholesterol homeostasis exists under balance between intracellular biosynthesis and uptake from extracellular origin by cell surface transport proteins. Expected role of cholesterol on either tumor suppressor gene and/or DNA synthesis has been aimed in the present study to explore intracellular cholesterol homeostasis in CLL subjects. Higher expressions of p53R2 (p53 dependent subunit of ribonucleotide reductase) and p53 were found in lymphocytes of chronic human lymphocytic leukemia as comparison to their normal counterparts. Inverse relation was found with p53 independent R2 subunit (in human hRRM2) of ribonucleotide reductase, which was found to be decreased from its control group. More expression of peripheral type benzodiazepine receptor, a cholesterol transporter, was noticed in isolated nuclear fraction with simultaneous increase of cholesterol concentration in cytoplasmic and nuclear compartments. A parallel increase of cholesterol in cell nucleus with increased p53R2 expression shows priority of the involvement of cholesterol in the process of cell replication.

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Funding

This study was funded by AIIMS internal grant. Grant number: F.6-1/2008-Acad. (PM).

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Author notes

  1. Ankit Verma

    Present address: CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi, India

  2. N. C. Chandra

    Present address: All India Institute of Medical Sciences (AIIMS), Phulwari Sharif, Patna, Bihar, 801507, India

Authors and Affiliations

  1. All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi, 110029, India

    Ankit Verma & N. C. Chandra

Authors
  1. Ankit Verma
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  2. N. C. Chandra
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Correspondence to N. C. Chandra.

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Conflict of interest

Ankit Verma declares that he has no conflict of interest. N. C. Chandra declares that he has no conflict of interest.

Human and Animal Rights

The study was performed using human blood samples. The participants included were chronic lymphocytic leukemia patients and age-matched healthy controls.

Informed Consent

Informed consent was obtained from all individual participants included in the study.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Ethics approval reference number: IESC/T-66/03.02.2012.

Additional information

The work was done completely in Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi-110029.

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Verma, A., Chandra, N.C. Differential Expressions of p53, p53R2, hRRM2 and PBR in Chronic Lymphocytic Leukemia: A Correlation with Intracellular Cholesterol. Ind J Clin Biochem 31, 336–341 (2016). https://doi.org/10.1007/s12291-015-0539-4

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  • DOI: https://doi.org/10.1007/s12291-015-0539-4

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