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Indian Journal of Clinical Biochemistry

, Volume 31, Issue 1, pp 117–120 | Cite as

Carbohydrate Deficient Transferrin and Interleukin-6 as Predictors of Fibrosis in Alcohol Cirrhosis

  • Hanumanthappa NandeeshaEmail author
  • Medha Rajappa
  • Tamilarasu Kadhiravan
  • Krishnamoorthy Srilatha
  • Kottyen Thazhath Harichandrakumar
  • Durgadevi Thyagarajan
Short Communication

Abstract

The severity of alcoholic cirrhosis depends on the presence of liver inflammation and fibrogenesis. Previous studies have hypothesized that carbohydrate deficient transferrin can be used as marker of liver impairment in alcoholic liver disease patients. The present study was designed to assess whether carbohydrate deficient transferrin is associated with procollagen III peptide and predict fibrosis in alcohol cirrhosis patients. We enrolled 48 patients with alcoholic cirrhosis and 38 healthy controls. Serum carbohydrate deficient transferrin, procollagen III peptide and interleukin-6 levels were estimated in both groups. Serum carbohydrate deficient transferrin, procollagen III peptide and interleukin-6 were significantly increased in alcoholic cirrhosis patients compared to controls. Stepwise regression analysis showed that carbohydrate deficient transferrin (adjusted R2 = 0.313, β = 0.362, p = 0.003) and interleukin-6 (adjusted R2 = 0.194, β = 0.459, p = 0.001) were positively associated with procollagen III peptide when age, duration and amount of alcohol consumption were considered as covariates. We conclude that elevated carbohydrate deficient transferrin and interleukin-6 act as predictors of fibrosis in alcoholic cirrhosis.

Keywords

Carbohydrate deficient transferrin Procollagen III peptide Interleukin-6 Inflammation Fibrogenesis 

Abbreviations

CDT

Carbohydrate deficient transferrin

MELD

Model for end stage liver disease

IL-6

Interleukin-6

INR

International normalized ratio

PCIIIP

Procollagen III peptide

Notes

Acknowledgments

This work was supported by a grant from JIPMER intramural fund sanctioned to the corresponding author.

Compliance with Ethical Standards

Conflict of interest

None.

References

  1. 1.
    Das SK, Balakrishnan V, Vasudevan DM. Alcohol: its health and social impact in India. Natl Med J India. 2006;19:94–9.PubMedGoogle Scholar
  2. 2.
    Miranda-Mendez A, Lugo-Baruqui A, Armendariz-Borunda J. Molecular basis and current treatment for alcoholic liver disease. Int J Environ Res Public Health. 2010;7:1872–88.PubMedCentralCrossRefPubMedGoogle Scholar
  3. 3.
    Frankel E, Szabo G, Lengyel G, et al. Does carbohydrate-deficient transferrin have diagnostic value in non-alcoholic fatty liver disease? Orvosi Hetil. 2009;150(31):1471–5.CrossRefGoogle Scholar
  4. 4.
    DiMartini A, Day N, Lane T, Beisler AT, Dew MA, Anton R. Carbohydrate deficient transferrin in abstaining patients with end-stage liver disease. Alcohol Clin Exp Res. 2001;25(12):1729–33.PubMedCentralCrossRefPubMedGoogle Scholar
  5. 5.
    Poynard T, Mathurin P, Lai CL, et al. A comparison of fibrosis progression in chronic liver diseases. J Hepatol. 2003;38:257–65.CrossRefPubMedGoogle Scholar
  6. 6.
    Lieber CS, Weiss DG, Paronetto F. Value of fibrosis markers for staging liver fibrosis in patients with precirrhotic alcoholic liver disease. Alcohol Clin Exp Res. 2008;32(6):1031–9.CrossRefPubMedGoogle Scholar
  7. 7.
    Gressner OA, Weiskirchen R, Gressner AM. Biomarkers of liver fibrosis: clinical translation of molecular pathogenesis or based on liver-dependent malfunction tests. Clin Chim Acta. 2007;381(2):107–13.CrossRefPubMedGoogle Scholar
  8. 8.
    Kamath PS, Kim WR. The model for end-stage liver disease (MELD). Hepatology. 2007;45(3):797–805.CrossRefPubMedGoogle Scholar
  9. 9.
    Anttlla P, Jarvi K, Latvala J, Blake JE, Niemela O. Characteristics of different carbohydrate deficient transferrin methods for the detection of problem drinking: effects of liver disease and alcohol consumption. Alcohol. 2003;38:415–20.CrossRefGoogle Scholar
  10. 10.
    Welker MW, Printz H, Hackler R, et al. Identification of elevated carbohydrate deficient transferrin serum level as transferrin D-variant by means of isoelectric focusing. Z Gastroenterol. 2004;42:1049–54.CrossRefPubMedGoogle Scholar
  11. 11.
    Renau-Piqueras J, Guasch R, Azorin I. Prenatal alcohol exposure affects galactosyltransferase activity and glycoconjugates in the golgi apparatus of fetal rat hepatocytes. Hepatology. 1997;25:343–50.CrossRefPubMedGoogle Scholar
  12. 12.
    Plebani M, Burlina A. Biochemical markers of hepatic fibrosis. Clin Biochem. 1991;24(3):219–39.CrossRefPubMedGoogle Scholar
  13. 13.
    Lee UE, Friedman SL. Mechanism of hepatic fibrogenesis. Best Pract Res Clin Gastroenterol. 2011;25(2):195–206.PubMedCentralCrossRefPubMedGoogle Scholar
  14. 14.
    Kliaritskaia IL, Stilidi EI. Pathogenetic importance of proinflammatory cytokines in the formation and progression of fibrosis in alcoholichepatitis. Eksp Klin Gastroenterol. 2013;4:13–20.PubMedGoogle Scholar
  15. 15.
    El-Assal O, Hong F, Kim WH, Radaeva S, Gao B. IL-6-deficient mice are susceptible to ethanol induced hepatic steatosis: IL-6 protects against ethanol-induced oxidative stress and mitochondrial permeability transition in the liver. Cell Mol Immunol. 2004;1(3):205–11.PubMedGoogle Scholar
  16. 16.
    Fukumura A, Tsutsumi M, Tsuchishima M, et al. Effect of the inducer of interleukin-6 (ME3738) on rat liver treated with ethanol. Alcohol Clin Exp Res. 2007;31:S49–53.CrossRefPubMedGoogle Scholar
  17. 17.
    Swiatkowska-Stodulska R, Bakowska A. Serum interleukin-6 concentrations in patients with alcoholic liver disease. Pol Merkur Lekarski. 2004;17(99):255–9.PubMedGoogle Scholar
  18. 18.
    Torres-Salinas M, Parés A, Caballería J, Jiménez W, Heredia D, Bruguera M, et al. Serum procollagen type III peptide as a marker of hepatic fibrogenesis in alcoholic hepatitis. Gastroenterology. 1986;90:1241–6.PubMedGoogle Scholar
  19. 19.
    Trinchet JC, Hartmann DJ, Pateron D, Munz-Gotheil C, Callard P, Ville G, et al. Serum type I collagen and N-terminal peptide of type III procollagen in patients with alcoholic liver disease: relationship to liver histology. Alcohol Clin Exp Res. 1992;16(2):342–6.CrossRefPubMedGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2015

Authors and Affiliations

  • Hanumanthappa Nandeesha
    • 1
    Email author
  • Medha Rajappa
    • 1
  • Tamilarasu Kadhiravan
    • 2
  • Krishnamoorthy Srilatha
    • 1
  • Kottyen Thazhath Harichandrakumar
    • 3
  • Durgadevi Thyagarajan
    • 1
  1. 1.Department of BiochemistryJawaharlal Institute of Post Graduate Medical Education and ResearchPuducherryIndia
  2. 2.Department of MedicineJawaharlal Institute of Post Graduate Medical Education and ResearchPuducherryIndia
  3. 3.Department of Medical Biometrics and InformaticsJawaharlal Institute of Post Graduate Medical Education and ResearchPuducherryIndia

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