Skip to main content
SpringerLink
Log in

Study of Association of Leptin and Insulin Resistance Markers in Patients of PCOS

  • Original Article
  • Published:
Indian Journal of Clinical Biochemistry Aims and scope Submit manuscript

Abstract

Polycystic ovary syndrome (PCOS) is a common endocrinological disorder among women of the reproductive age group with long term sequelae which include diabetes mellitus, hypertension and CAD. The present study was conducted to evaluate the association of leptin—an adipokine playing an important role in carbohydrate metabolism and markers of insulin resistance among women with PCOS. Sixty diagnosed cases of PCOS as per Rotterdam criteria were enrolled in this study after informed written consent. Insulin resistance was estimated using the homeostatic model assessment-insulin resistance (HOMA-IR). HOMA-IR was calculated as the product of the fasting plasma insulin value (mU/ml) and the fasting plasma glucose value (mg/dl), divided by 405 and HOMA β was calculated as 360 × [insulin]/([glucose] − 63) % (glucose in mg/dl). Estimation of serum leptin levels was done by ELISA using leptin ELISA kit from (DRG). A positive correlation of serum leptin levels was observed with markers of insulin resistance. Multiple regression analysis with HOMA-IR as dependent variable demonstrated a statistically significant contribution of fasting insulin levels. This study highlights the role of leptin in alterations in carbohydrate metabolism in patients with PCOS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Stein I, Leventhal M. Amenorrhea associated with bilateral polycystic ovaries. Am J Obstet Gynecol. 1935;29:181.

    Article  Google Scholar 

  2. Azziz R, Woods KS, Reyna R, Key TJ, Knochenhauer ES, Yildiz BO. The prevalence and features of the polycystic ovary syndrome in an unselected population. J Clin Endocrinol Metab. 2004;89:2745–9.

    Article  CAS  PubMed  Google Scholar 

  3. Zawadzki J, Dunaif A. Diagnostic criteria for polycystic ovary syndrome: towards a rational approach. In: Dunaif A, Givens JR, Haseltine FP, Merriam GR, editors. Polycystic ovary syndrome. Oxford: Blackwell Scientific Publications; 1992. p. 377–84.

    Google Scholar 

  4. Franks S. Polycystic ovary syndrome. N Engl J Med. 1995;333:853–61.

    Article  CAS  PubMed  Google Scholar 

  5. Hoeger KM. Role of lifestyle modification in the management of polycystic ovary syndrome. Best Pract Res Clin Endocrinol Metab. 2006;20:293.

    Article  PubMed  Google Scholar 

  6. Zhang YY, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homolog. Nature. 1994;372:425–32.

    Article  CAS  PubMed  Google Scholar 

  7. Leroy P, Dessolin S, Villageois P, Moon BC, Friedman JM, Ailhaud G, et al. Expression of ob gene in adipose cells and regulation by insulin. J Biol Chem. 1996;271:2365–8.

    Article  CAS  PubMed  Google Scholar 

  8. Brzechffa PR, Jakımıuk AJ, Agarwal SK, Weitsman SR. Serum immunoreactive leptin concentrations in women with polycystic ovary syndrome. J Clin Endocrinol Metab. 1996;84:4166–9.

    Google Scholar 

  9. Mendonça HC, Montenegro RM, Foss MC, Silva De Sa MF, Ferriani RA. Positive correlation of serum leptin with estradiol levels in patients with polycystic ovary syndrome. Braz J Med Biol Res. 2004;37:729–36.

    Article  PubMed  Google Scholar 

  10. Fritz MA, Speroff L. Clinical gynaecology endocrinology and infertility. 8th ed. New delhi: Wolters Klumer, Lippikott Williams, Wilkins Publisher; 2011.

    Google Scholar 

  11. Godarzi MO, Korenman SG. The importance of insulin resistance in polycystic ovary syndrome. Fertil Steril. 2003;80:255–8.

    Article  Google Scholar 

  12. Mancini F, Cianciosi A, Marchesini RG, Facchinetti F, Cesare B, Aloysio D. Endothelial function and its relationship to leptin, homocysteine, and insulin resistance in lean and overweight eumenorrheic women and polycystic patients. Fertil Steril. 2009;91:2537–44.

    Article  CAS  PubMed  Google Scholar 

  13. Erturk E, Kuru N, Savci Tuncel E, Ersoy C, Imamoglu S. Serum leptin levels correlate with obesity parameters but not with hyperinsulinism in women with polcystic ovary syndrome. Fertil Steril. 2004;82:1364–8.

    Article  PubMed  Google Scholar 

  14. Remsberg KE, Talbott EO, Zborowski JV, Evans RW, Pemu K, et al. Evidence for competing effects of body mass, hyperinsulinemia, insulin resistance, and androgens on leptin levels among lean, overweight, and obese women with polycystic ovary syndrome. Fertil Steril. 2002;78:479–86.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Lady Hardinge Medical College, New Delhi, India

    Huma Nasrat, Surajeet K. Patra, Binita Goswami & Anju Jain

  2. Department of Obstetrics & Gynecology, Lady Hardinge Medical College, New Delhi, India

    Chitra Raghunandan

Authors
  1. Huma Nasrat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Surajeet K. Patra
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Binita Goswami
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anju Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chitra Raghunandan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Binita Goswami.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nasrat, H., Patra, S.K., Goswami, B. et al. Study of Association of Leptin and Insulin Resistance Markers in Patients of PCOS. Ind J Clin Biochem 31, 104–107 (2016). https://doi.org/10.1007/s12291-015-0499-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12291-015-0499-8

Keywords

Search

Navigation