Skip to main content
Log in

Evaluation of Serum Vitamin B12 Levels and Its Correlation with Anti-Thyroperoxidase Antibody in Patients with Autoimmune Thyroid Disorders

  • Original Article
  • Published:
Indian Journal of Clinical Biochemistry Aims and scope Submit manuscript


Vitamin B12 deficiency has been reported in patients with Autoimmune thyroid disorders. However there is limited data on exact prevalence of low B12 and its correlation with anti-thyroperoxidase antibody (anti-TPO) levels in these patients. The aim of our study was to estimate serum vitamin B12 levels in autoimmune thyroid disorders and to correlate B12 levels with anti-TPO. 350 patients were selected by convenient sampling. Vitamin B12 levels and thyroid parameters were estimated using fully automated chemiluminescence method on Access 2. Results of our study shows that using the manufacturer’s cut-off of 145 pg/mL, the prevalence of low serum vitamin B12 was found to be 45.50 %. Higher prevalence (55 %) was seen based on the published cut-off of 200 pg/mL The study however did not demonstrate any significant correlation between vitamin B12 levels and anti-TPO (r = −0.11 and p value of 0.30).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others


  1. Marcocci C, Chiovato L. Thyroid-directed antibodies. In: Braverman LE, Utiger RD, editors. The thyroid: a fundamental and clinical text. 8th ed. Philadelphia: Lippincott Williams & Wilkins; 2000. p. 414–31.

    Google Scholar 

  2. Fein HG, Rivlin RS. Anemia in thyroid disease. Med Clin N Am. 1975;59:1133–45.

    CAS  PubMed  Google Scholar 

  3. Caplan RH, Davis K, Bengstone B, Smith MJ. Serum folate and vitamin B12 levels in hypothyroid and hyperthyroid patients. Arch Intern Med. 1975;135:701–4.

    Article  CAS  PubMed  Google Scholar 

  4. Ness-Aabramof R, Nabriski DA, Braverman LE, Shilo L, Weiss E, Reshef T, et al. Prevalence and evaluation of B12 deficiency in patients with autoimmune thyroid disease. Am J Med Sci. 2006;332:119–22.

    Article  Google Scholar 

  5. Jabbar A, Yawar A, Waseem S, Islam N, Ul Haque N, Zuberi L, et al. Vitamin B12 deficiency common in primary hypothyroidism. J Pak Med Assoc. 2008;58(5):258–61.

    PubMed  Google Scholar 

  6. Kankonkar SR, Joshi SV, Tijoriwala SJ, Prabhu RV, Raikar SAC, Kankonkar RC et al. Study of vitamin B12 deficiency in different disease.

  7. Lippi G, Montagnan M, Targher G, Salvagno GI, Guidi GC. Prevalence of folic acid and vitamin B12 deficiencies in patients with thyroid disorders. Am J Med Sci. 2008;336(1):50–2.

    Article  PubMed  Google Scholar 

  8. Stabler SP, Allen RH, Savage DG, Lindenbaum J. Clinical spectrum and diagnosis of cobalamin deficiency. Blood. 1990;76:871–81.

    CAS  PubMed  Google Scholar 

  9. Baik HW, Russell RM. Vitamin B12 deficiency in the elderly. Annu Rev Nutr. 1999;19:357–77.

    Article  CAS  PubMed  Google Scholar 

  10. Beckman Coulter Inc. Access vitamin B12 product insert, Fullerton, WB A33437B.

  11. Carmel R. Biomarkers of cobalamin (vitamin B12) status in the epidemiological setting: a critical overview of context, applications and performance characteristics of cobalamin, MMA and holotranscobalamin 11. Am J Clin Nutr. 2011;94:348S–58S.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  12. Beckman Coulter.Inc. Access TPO antibody product insert, Fullerton, WB A35485B.

  13. Perros P, Singh RK, Ludlam CA, Frier BM. Prevalence of pernicious anemia in patients with type 1 diabetes and autoimmune thyroid disorders. Diabet Med. 2000;17:749–51.

    Article  CAS  PubMed  Google Scholar 

  14. Hvas AM, Nexo E. Diagnosis and treatment of vitamin B12 deficiency-an update. Haematologica. 2006;91:1506–12.

    CAS  PubMed  Google Scholar 

Download references


The authors would like to thank the Research Society, St John’s Medical College Hospital for providing the grant for the study.

Author information

Authors and Affiliations


Corresponding author

Correspondence to S. Jaya Kumari.

Electronic Supplementary Material

Below is the link to the electronic supplementary material.

Supplementary material 1 (TIFF 16,451 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jaya Kumari, S., Bantwal, G., Devanath, A. et al. Evaluation of Serum Vitamin B12 Levels and Its Correlation with Anti-Thyroperoxidase Antibody in Patients with Autoimmune Thyroid Disorders. Ind J Clin Biochem 30, 217–220 (2015).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: