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Emerging Trend of Mutation Profile of rpoB Gene in MDR Tuberculosis, North India

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Abstract

The present study was conducted on North Indian population to observe rpoB gene mutation profile in multidrug resistant Mycobacterium tuberculosis. This was an observational study. 30 cases of MDR-TB proven by culture and drug sensitivity were selected. DNA sequencing of 81 bp (codon 507–533) long RRDR of Mycobacterium tuberculosis was done to detect the sites of mutation. Out of 30 cases, 24 showed a single mutation in the RRDR region of rpoB gene in which 16 (53.33 %) showed mutation in codon 531(TCG→TTG), 5 cases (16.66 %) showed mutation in codon 526(CAC→TAC), mutation in codon 516(GAC→GTC, AAC) was present in 3 cases (10 %). It was also observed that mutation in more than one codon was present in 4 cases (13.33 %), which included deletion at codon 509(AGC→–GC), mutation at 513(CAA→CTA), 516, 526, 529(CGA→CTA) and 531. No mutation was detected in RRDR in 2 cases (6.66 %). Our finding of 13.33 % cases with multiple sites of mutation in RRDR region is in contrast to earlier studies done in North India which showed single mutation detected in RRDR of rpoB gene that highlights the emerging change in the trend of mutation profile of rpoB gene in rifampicin resistant Mycobacterium tuberculosis.

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Acknowledgments

The study was supported by grant from Tapedic Unmolan Samiti, Government of NCT, New Delhi, India.

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Authors and Affiliations

  1. Department of Biochemistry, Lady Hardinge Medical College, New Delhi, India

    Jemil S. Makadia, Anju Jain & Surajeet Kumar Patra

  2. Department of Microbiology, Lady Hardinge Medical College, New Delhi, India

    B. L. Sherwal

  3. Department of Chest Clinic, Lok Nayak Hospital, New Delhi, India

    Ashwani Khanna

Authors
  1. Jemil S. Makadia
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  2. Anju Jain
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  3. Surajeet Kumar Patra
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  4. B. L. Sherwal
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  5. Ashwani Khanna
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Correspondence to Anju Jain.

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Makadia, J.S., Jain, A., Patra, S.K. et al. Emerging Trend of Mutation Profile of rpoB Gene in MDR Tuberculosis, North India. Ind J Clin Biochem 27, 370–374 (2012). https://doi.org/10.1007/s12291-012-0228-5

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  • DOI: https://doi.org/10.1007/s12291-012-0228-5

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