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Quantitative Assessment of Expression of Lactate Dehydrogenase and its Isoforms 3 and 4 may Serve as Useful Indicators of Progression of Gallbladder Cancer: A Pilot Study

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Abstract

We have studied the expression of lactate dehydrogenase and its isoforms in gall bladder cancer, cholelithiasis and chronic cholecystitis. Quantitative and qualitative assays of lactate dehydrogenase and its various isoforms were carried out in the blood sera of patients and healthy controls along with parallel estimation of various liver function test enzymes. Statistical analysis was done using the software Graph Pad Prism. Significantly high expression of lactate dehydrogenase along with alkaline phosphatase and total bilirubin (P ≤ 0.05) was observed in all the three clinical conditions as compared to controls. LDH showed an increasing trend from stage I to stage IV of GBC indicating a significant positive association with the disease progression. The levels of LDH 3 and 4 isoforms appeared significantly more elevated in GBC than cholelithiasis or chronic cholecystitis. We suggest that a careful estimation of total LDH and its isoforms 3 and 4 alone or along with alkaline phosphatase and total bilirubin during different clinical stages, like chronic cholecystitis, cholelithiasis and GBC, may prove to be a potentially useful biomarker in the prognostic management of gall bladder diseases, specifically GBC.

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Acknowledgements

We acknowledge financial support of Department of Biotechnology, Government of India to Centre for Genomics, Jiwaji University. We also acknowledge the help extended by Dr. Manisha Kakkar during sample collection. MAB is a recipient of Senior Research Fellowship from Indian Council of Medical Research. TDS is grateful to JU for research fellowship.

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Authors and Affiliations

  1. Centre for Genomics, Molecular and Human Genetics, School of Studies in Zoology, Jiwaji University, Gwalior, 474011, India

    Tekcham Dinesh Singh, Mustafa Ahmed Barbhuiya & Pramod Kumar Tiwari

  2. Department of Pathology, Cancer Hospital and Research Institute (CHRI), Gwalior, 474009, India

    Sanjeev Gupta

  3. Department of Surgery, Cancer Hospital and Research Institute (CHRI), Gwalior, 474009, India

    Braj Raj Shrivastav, Viswajit Jalaj & Nidisha Agarwal

  4. Department of Surgery, Gajra Raja Medical College (GRMC), Gwalior, 474009, India

    Braj Raj Shrivastav

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  1. Tekcham Dinesh Singh
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  2. Mustafa Ahmed Barbhuiya
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Correspondence to Pramod Kumar Tiwari.

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Singh, T.D., Barbhuiya, M.A., Gupta, S. et al. Quantitative Assessment of Expression of Lactate Dehydrogenase and its Isoforms 3 and 4 may Serve as Useful Indicators of Progression of Gallbladder Cancer: A Pilot Study. Ind J Clin Biochem 26, 146–153 (2011). https://doi.org/10.1007/s12291-011-0117-3

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  • DOI: https://doi.org/10.1007/s12291-011-0117-3

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