Skip to main content

Potential Effect of Bacopa monnieri on Nitrobenzene Induced Liver Damage in Rats

Indian Journal of Clinical Biochemistry volume 25, pages 401–404 (2010)Cite this article

Abstract

The study was designed to evaluate the hepatoprotective activity of ethanolic extract of Bacopa monnieri in acute experimental liver injury induced by Nitrobenzene in rats. The extract at the dose of 200 mg/kg body weight was administered orally once every day for 10 days. The increased serum marker enzymes, Aspartate transaminase, Alanine transaminase and alkaline phosphatase were restored towards normalization significantly by the extract. Significant increase in SOD, CAT and GPx was observed in extract treated liver injured experimental rats. Histopathological examination of the liver tissues supported the hepatoprotection. It is concluded that the ethanolic extract of Bacopa monieri plant possess good hepatoprotective activity.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. World Health Organization. WHO Traditional Medicine Strategy 2002–2005. Geneva: World Health Organization; 2002.

    Google Scholar 

  2. Zang X. Traditional medicine: its importance and protection. In: Twarog S, Kapoor P, editors. Protecting and promoting traditional knowledge: systems, national experiences and international dimensions. Part I. The role of traditional knowledge in health care and agriculture. New York: United Nations; 2004. p. 3–6.

    Google Scholar 

  3. Kapoor LD. Handbook of ayurvedic medicinal plants. Baca Raton, FL: CRC Press; 1990. p. 317–29.

    Google Scholar 

  4. Chopra RN, Chopra IC, Verma BS. Supplementary to glossary of Indian medicinal plants. New Delhi: National Institute of Science Communication, Council of Scientific and Industrial Research; 1998. p. 10–1.

    Google Scholar 

  5. Russo A, Borelli F. Bacopa monniera, a reputed nootropic plant: an overview. Phytomedicine. 2005;12:305–17.

    Article  CAS  PubMed  Google Scholar 

  6. Vohora D, Pal SN, Pillai KK. Protection from phenytoin-induced cognitive deficit by Bacopa monniera, a reputed Indian nootropic plant. J Ethnopharmacol. 2000;71:383–90.

    Article  CAS  PubMed  Google Scholar 

  7. Kishore K, Singh M. Effect of bacosides, a alcoholic extract of Bacopa monnieri Linn. (brahmi), on experimental amnesia in mice. Indian J Exp Biol. 2005;43:640–5.

    PubMed  Google Scholar 

  8. Anbarasi K, Vani G, Balakrishna K, Desai CS. Creatine kinase isoenzyme patterns upon exposure to cigaratte smoke: protective effect of bacoside A. Vasc Pharmacol. 2005;42:57–61.

    Article  CAS  Google Scholar 

  9. Deepak M, A Amit. The need for establishing identities of bacoside A, and B, the putative major bioactive saponins of Indian medicinal plant Bacopa monnieri. Phytomedicine. 2004;11:264–8.

    Article  CAS  PubMed  Google Scholar 

  10. Cattley RC, Everitt JI, Gross EA. Carcinogenicity and toxicity of inhaled nitrobenzene in B6C3F1 mice and F344 and CD rats. Toxicol Sci. 1994;22:328–40.

    Article  CAS  Google Scholar 

  11. Howard PC, Beland FA, Cerniglia CE. Reduction of the carcinogen 1-nitropyrene to 1-aminopyrene by the rat intestinal bacteria. Carcinogenesis. 1983;4:985–90.

    Article  CAS  PubMed  Google Scholar 

  12. International Programme on Chemical Safety (IPCS). Nitrobenzene environmental health criteria 230. Geneva: WHO; 2003.

    Google Scholar 

  13. King EJ. Practical clinical Enzymol. London: D. Van Nostrand Co.; 1965.

    Google Scholar 

  14. King EJ, Armstrong AR. Calcium, magnesium, phosphorus and phosphatase. In: Varley B, Gowenlock AH, Bell M, editors. Practical clinical biochemistry, vol. 1. London: Heinemann; 1980. p. 850–2.

    Google Scholar 

  15. Misra HP, Fridovich I. The role of superoxide anion in the antioxidation of epinephrine and simple assay of superoxide dismutase. J Biol Chem. 1972;247:3170–5.

    CAS  PubMed  Google Scholar 

  16. Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972;47:389–94.

    Article  CAS  PubMed  Google Scholar 

  17. Rotruck JT, Pope AL, Ganther HE. Selenium, biochemical role as a component of glutathione peroxidase purification and assay. Science. 1973;179:588–90.

    Article  CAS  PubMed  Google Scholar 

  18. Hogberg J, Larson RE, Kristoferson A, Orrhenices S. NADPH-dependent reductase solubilised from microsomes by peroxidation and its activity. Biochem Biophys Res Commun. 1974;56:836–42.

    Article  CAS  PubMed  Google Scholar 

  19. Janani P, Sivakumari K, Parthasarathy C. Hepatoprotective activity of bacoside A against N-nitrosodiethylamine-induced liver toxicity in adult rats. Cell Biol Toxicol. 2008;24:471–4.

    Article  Google Scholar 

  20. Holder JW. Nitrobenzene potential human cancer risk based on animal studies. Toxicol Ind Health. 1999;15:458–63.

    CAS  PubMed  Google Scholar 

  21. Mason RP, Holtzman JL. The role of catalytic superoxide formation in O2 inhibition of nitroreductase. Biochem Biophys Res Commun. 1975;67:1267–74.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We, the authors are thankful to our Chancellor, Chief Executive Officer, Vice Chancellor and Registrar of Karpagam University for providing facilities and encouragement.

Author information

Authors and Affiliations

  1. Department of Biochemistry, Karpagam University, Coimbatore, 641021, India

    B. Rajalakshmy Menon, M. A. Rathi, L. Thirumoorthi & V. K. Gopalakrishnan

Authors
  1. B. Rajalakshmy Menon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Rathi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Thirumoorthi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. K. Gopalakrishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. K. Gopalakrishnan.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Menon, B.R., Rathi, M.A., Thirumoorthi, L. et al. Potential Effect of Bacopa monnieri on Nitrobenzene Induced Liver Damage in Rats. Ind J Clin Biochem 25, 401–404 (2010). https://doi.org/10.1007/s12291-010-0048-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12291-010-0048-4

Keywords

  • Hepatoprotective effect
  • Antioxidants
  • Liver marker enzymes