Indian Journal of Clinical Biochemistry

, Volume 25, Issue 4, pp 393–397 | Cite as

Effect of Turmeric and its Active Principle Curcumin on T3-Induced Oxidative Stress and Hyperplasia in Rat Kidney: A Comparison

  • Luna SamantaEmail author
  • Jogamaya Panigrahi
  • Shravani Bhanja
  • Gagan B. N. Chainy
Original Article


The present study was designed to compare the potential of turmeric and its active principle curcumin on T3-induced oxidative stress and hyperplasia. Adult male Wistar strain rats were rendered hyperthyroid by T3 treatment (10 μg · 100 g−1 · day−1 intraperitoneal for 15 days in 0.1 mM NaOH) to induce renal hyperplasia. Another two groups were treated similarly with T3 along with either turmeric or curcumin (30 mg kg−1 body weight day−1 orally for 15 days). The results indicate that T3 induces both hypertrophy and hyperplasia in rat kidney as evidenced by increase in cell number per unit area, increased protein content, tubular dilation and interstitial edema. These changes were accompanied by increased mitochondrial lipid peroxidation and superoxide dismutase activity without any change in catalase activity and glutathione content suggesting an oxidative predominance. Both turmeric and curcumin were able to restore the level of mitochondrial lipid peroxidation and superoxide dismutase activity in the present dose schedule. T3-induced histo-pathological changes were restored with turmeric treatment whereas curcumin administration caused hypoplasia. This may be due to lower concentration of curcumin in the whole turmeric. Thus it is hypothesized that regulation of cell cycle in rat kidney by T3 is via reactive oxygen species and curcumin reveres the changes by scavenging them. Although the response trends are comparable for both turmeric and curcumin, the magnitude of alteration is more in the later. Turmeric in the current dose schedule is a safer bet than curcumin in normalizing the T3-induced hyperplasia may be due to the lower concentration of the active principle in the whole spice.


Oxidative stress Kidney Curcumin Turmeric Hyperplasia Thyroid 



Financial assistance to the Departments from the Department of Biotechnology and University Grants Commission, Government of India is gratefully acknowledged.


  1. 1.
    Brouet I, Oshimima H. Curcumin, an antitumor promoter and anti-inflammatory agent, inhibits induction of nitric oxide synthase in activated macrophages. Biochem Biophys Res Commun. 1995;206:533–40.CrossRefPubMedGoogle Scholar
  2. 2.
    Duvoix A, Blassius R, Delhalle S, Schnekenburger M, Morceau F, Henry E, Dicato M, Diederich M. Chemopreventive and therapeutic effects of curcumin. Cancer Lett. 2005;233:181–90.CrossRefGoogle Scholar
  3. 3.
    Cohly HHP, Taylor A, Angel MF, Saahudeen AK. Effect of turmeric, turmerin and curcumin on H2O2 induced renal epithelia (LLC-PK1) cell injury. FRBM. 1988;24:49–54.CrossRefGoogle Scholar
  4. 4.
    Mano T, Sinohara R, Sawai Y, Oda N, Nishida Y, Mokunu T, Kotake M, Hamada M, Masunaga R, Nakai A, Nagasaka A. Effects of thyroid hormne on coenzyme Q and other free radical scavengers in rat heart muscle. J Endocrinol. 1995;145:131–6.CrossRefPubMedGoogle Scholar
  5. 5.
    Sahoo DK, Roy A, Bhanja S, Chainy GBN. Experimental hypothyroidism induced oxidative stress and impairment of antioxidant defence system in rat testis. Indian J Exp Biol. 2005;43:1058–67.PubMedGoogle Scholar
  6. 6.
    Vendilti P, Balestrieri M, Dimeo S, Deleo T. Effect of thyroid state on lipid peroxidation, antioxidant defences and susceptibility to oxidative stress in rat tissues. J Endocrinol. 1997;155:151–7.CrossRefGoogle Scholar
  7. 7.
    Wilcox CS. Reactive oxygen species: roles in blood pressure and kidney function. Curr Hyperten Rep. 2002;4:160–6.CrossRefGoogle Scholar
  8. 8.
    Stephen F, Reville P, de Laharpe F, Koll-Back MH. Impairment of renal hypertrophy by hypothyroidism in the rat. Life Sci. 1982;30:623–31.CrossRefGoogle Scholar
  9. 9.
    Ohmura T, Katyal SL, Locker J, Ledda-Columbano GM, Columbano A, Shinozuka H. Induction of cellular DNA synthesis in the pancreas and kidneys of rats by peroxisome proliferators, 9-cis retinoic acid, and 3,3′,5-triiodo-lthyronine. Cancer Res. 1997;57:795–8.PubMedGoogle Scholar
  10. 10.
    Samanta L, Chainy GBN. Comparison of hexachlorocyclohexane induced oxidative stress in testis of immature and adult rats. Comp Biochem Physiol. 1997;118C:319–27.Google Scholar
  11. 11.
    Dorothy APL, Reinhard LL. Heart mitochondrial function in acute and in chronic hyperthyroidism in rats. Circ Res. 1969;25:171–81.Google Scholar
  12. 12.
    Lowry OH, Rrosebrough NJ, Farr AL, Randall RJ. Protein measurement with Folin phenol reagent. J Biol Chem. 1951;193:265–75.PubMedGoogle Scholar
  13. 13.
    Okhawa H, Ohisi N, Yagi K. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anals Biochem. 1979;95:351–8.CrossRefGoogle Scholar
  14. 14.
    Das K, Samanta L, Chainy GBN. A modified spectrophotometric assay of superoxide dismutase using nitrite formation by superoxide radicals. Indian J Biochem Biophys. 2000;37:201–4.Google Scholar
  15. 15.
    Aebi H. Catalase, In: Bergmeyer HU, editor. Methods in enzymatic analysis vol. 2 New York: Academic Press; 1974:673–8.Google Scholar
  16. 16.
    Cohen G, Dembiec D, Marcus J. Measurement of catalase activity in tissue extracts. Anals Biochem. 1970;34:30–8.CrossRefGoogle Scholar
  17. 17.
    Ellman GL. Tissue sulfydryl groups. Arch Biochem Biophys. 1959;82:70–7.CrossRefPubMedGoogle Scholar
  18. 18.
    Nakamura RM, Miyada DSM, Cockett AT, Moyer DL. Thyroid and pituitary gland activity during compensatory renal hypertrophy. Experientia, 1964;20:694–6.Google Scholar
  19. 19.
    Bradley SE, Stephen F, Coelho JB, Reville P. The thyroid and the kidney. Kidney Int. 1974;6:346–65.CrossRefPubMedGoogle Scholar
  20. 20.
    Mori T, Cowley AW. Renal oxidative stress in medullary twick ascending limbs produced by elevated NaCl and glucose. Hypertension. 2004;43:341–6.CrossRefPubMedGoogle Scholar
  21. 21.
    Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. New York: Oxford University Press; 2001.Google Scholar
  22. 22.
    Moreno JM, Rodrigue-Gomez I, Wangensteen R, Osuna A, Bueno P, Vragas F. Cardiac and renal antioxidant enzymes and effects of tempol in hyperthyroid rats. Am J Physiol 2005;289:E776–83.Google Scholar
  23. 23.
    Behrend L, Henderson G, Zwacka RM. Reactive oxygen species in oncogenic transformation. Biochem Soc Transact. 2003;31:1441–4.CrossRefGoogle Scholar
  24. 24.
    Apel K, Hirt H. Reactive oxygen species: metabolism, oxidative stress and signal transduction. Ann Rev Plant Biol. 2004;55:373–99.CrossRefGoogle Scholar
  25. 25.
    Sikora E, Bielak-Zmijewska A, Piwockq K, Skienski J, Radziszewska E. Inhibition of proliferation and apoptosis of human and rat T lymphocytes by curcumin, a curry pigment. Bio-chem Pharmacol. 1997;54:899–907.Google Scholar
  26. 26.
    Piwocka K, Jaruga EI, Skierski J, Gradzka I, Sikora E. Effect of glutathione depletion on caspase-3 independent apoptotic pathway induced by curcumin in Jurkat cells. FRBM. 2001;31:670–8.CrossRefGoogle Scholar
  27. 27.
    Soudamini KK, Unnikrishnan MC, Soni KB, Kuttan R. Inhibition of lipid peroxidation and cholesterol levels in mice by curcumin. Ind J Physiol Pharmacol. 1992;36:239–43.Google Scholar
  28. 28.
    Unnikrishnan MK, Rao MN. Inhibition of nitric oxide-induced oxidation of hemoglobin by curcuminoids. Pharmazie. 1995;50:490–2.PubMedGoogle Scholar
  29. 29.
    Sreejayan Rao MN. Curcuminoids as potent inhibitors of lipid peroxidation. J Pharmacol. 1994;46:1013–6.Google Scholar
  30. 30.
    Reddy ACP, Lokesh BR. Effect of dietary turmeric (Curcuma longa) on iron induced lipid peroxidaton in the rat liver. Food Chem Toxicol. 1994;32:279283.Google Scholar
  31. 31.
    Gilani AH, Shah AJ, Ghayur MN, Majeed K. Pharmacological basis for the use of turmeric in gastrointestinal and respiratory disorders. Life Sci. 2005;79:3089–105.CrossRefGoogle Scholar

Copyright information

© Association of Clinical Biochemists of India 2010

Authors and Affiliations

  • Luna Samanta
    • 1
    • 2
    Email author
  • Jogamaya Panigrahi
    • 2
  • Shravani Bhanja
    • 2
  • Gagan B. N. Chainy
    • 2
    • 3
  1. 1.Department of ZoologyRavenshaw UniversityCuttackIndia
  2. 2.Departments of Zoology and BiotechnologyUtkal UniversityBhubaneswarIndia
  3. 3.Imgenex India Pvt. Ltd., E-5, InfocityBhubaneswarIndia

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