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Oxidative stress in cases of chronic fluoride intoxication

Abstract

This study was conducted to find out the level of oxidative stress and effect of supplementation of vitamin C, D and Calcium on levels of SOD, serum and urinary fluoride in children residing in endemic fluorosis area. For this the fluoride belt of Jaipur district was selected. The parameters selected were Super oxide dismutase, serum fluoride and urinary fluoride. The study was conducted on one hundred children, selected from four areas (25 from each area) consuming water containing 1.2, 2.4, 5.6 and 13.6 mg/l of fluoride. Drinking water fluoride, serum and urinary fluoride were measured by Ion selective electrode method. Serum SOD by Xanthine oxidase method using kit of Ransod (kit cat. No. SD125). The post treatment values showed a significant reduction in serum fluoride and SOD. Urinary fluoride levels increased significantly in post treatment stage. The results revealed a normal SOD levels in all groups but an increasing trend was observed with increasing fluoride concentration. Treatment with Calcium, Vitamin D and Vitamin C showed a significant reduction in serum fluoride and SOD and increase in urinary fluoride. A high positive correlation between pretreatment and post treatment group was observed in serum fluoride, SOD and urinary fluoride (P < 0.05). The study indicated an increasing oxidative stress in cases of fluorosis with increasing drinking water fluoride concentration. Treatment with Calcium, Vitamin D and Vitamin C resulted a significant reduction in serum fluoride and SOD and increase in urinary fluoride.

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References

  1. YiamouvyIannis I. Fluoride: The ageing factor. Health Action Press, Delaware, OH, Chapter 14, 1993.

    Google Scholar 

  2. Waldbott GL, Burgstahler AW, McKinney HL. Fluoridation: The great dilemma. Coronado Press, Lawrence, Kensas 1978:pp 423.

    Google Scholar 

  3. Shivarajashankara YM, Shivashankara AR, Rao SH, Bhat PG. Oxidative stress in children with endemic skeletal fluorosis, Research Report 103. Fluoride 2001;34(2):103–107.

    Google Scholar 

  4. Akdogan M, Eraslan G, Gultekein F, Sahindokuyucu, Essizd D, Ankara, Isparta. Effect of fluoride on lipid peroxidation in rabbits, Fluoride Research report 185. Fluoride 2004;37(3):185–189.

    CAS  Google Scholar 

  5. Reddy GB, Khandare AL, Reddy PY, Rao GS, Balakrishna N, Srivalli I. Antioxidant defense system and lipid peroxidation in patients with skeletal fluorosis and in fluoride intoxicated rabbits. Toxicol Sci 2003;72(2):363–368.

    Article  CAS  PubMed  Google Scholar 

  6. Vani ML, Reddy KP. Effects of fluoride accumulation on some enzymes of brain and gastrocnemeus muscle of mice, Fluoride Research report 17. Fluoride 2000;33(1):17–26.

    CAS  Google Scholar 

  7. Li J, Cao S. Recent studies on endemic fluorosis in China. Fluoride 1994;27(4):125–128.

    Google Scholar 

  8. Reddy GS, Srikantia SG. Effect of dietary calcium, vitamin C and protein in development of experimental skeletal fluorosis, I growth, serum chemistry and changes in composition and radiological appearance of bone. Metabolism 1971;20:642–656.

    Article  CAS  PubMed  Google Scholar 

  9. Ming HO YU, Huey Lin Hwang. Influence of protein and ascorbic acid on fluoride induced changes in blood composition and skeletal fluoride deposition in mice. In: Fluoride Research 1985, Studies in environmental science vol. 27, Elsevier Science publisher BV, Amsterdam. 1988:203–210.

    Google Scholar 

  10. Gupta SK, Gupta RC, Seth AK, Gupta A. Reversal of fluorosis in children. Acta Peadiatrica Japonica 1996;38:513–519.

    CAS  Google Scholar 

  11. Susheela AK, Bhatnagar M. Reversal of fluoride induced cell injury through elimination of fluoride and consumption of diet rich in essential nutrients and antioxidants. Mol Cell Biochem 2002;234–235(1–2):335–340.

    Article  PubMed  Google Scholar 

  12. Kellogg P, Fridovich. Superoxide, H2O2 and single oxygen in lipid peroxidation by xanthine oxidase system. J Biol Chem 1975;250:8812–8817.

    CAS  PubMed  Google Scholar 

  13. Fuchs C, Dom D, Fuchs CA, Henning HV, Meintosh C, Scheler F. Fluoride determination in plasma by ion selective electrode: a simplified method for the clinical laboratory. Clin Chim Acta 1975;60:157–167.

    Article  CAS  PubMed  Google Scholar 

  14. APHA, Standard Methods for the Examination Water and Waste Water, 17th edition, AWWA, Water Pollution Control Federation, 1989.

  15. Delles C, Schneider MP, Oehmer S, Fleischmann I, Fleischmann EF, Schmieder RE. Increased response of renal perfusion to the antioxidant vitamin C in type 2 diabetes. Nephrol Dialysis Transplantation 2004;19(10):2513–2518.

    Article  CAS  Google Scholar 

  16. Fuleihan GEH, Seifter J, Scott J, Brown EM. Calcium-Regulated Renal Calcium Handling in Healthy Men: Relationship to Sodium Handling. J Clin Endocrinol Metabol 1998;83(7):2366–2372.

    Article  Google Scholar 

  17. Dinman BD, Bovard WJ, Bonney TB, Cohen JM, Colwell MO. Absorption and excretion of fluoride immediately after exposure, Part I. J Occupational Med 1976a;18:7–13.

    CAS  Google Scholar 

  18. Teotia M, Teotea SP, Singh KP. Endemic chronic fluoride toxicity and dietary calcium deficiency interaction syndromes of metabolic bone diseases & deformities in India in year 2000. Ind J Paedr 1998;65(3):371–381.

    Article  CAS  Google Scholar 

  19. Gupta SK, Gupta RC, Gupta K, Trivedi HP. Changes in serum seromucoid following compensatory Hyperparathyroidism: a sequel to chronic fluoride ingestion. Ind J Clin Biochem 2008;23(2):176–180.

    Article  CAS  Google Scholar 

  20. Birkner E, Grucka-Mamczar E, Kasperczyk S, Zalejska FJ, Kasperczyk A, et al. The influence of sodium fluoride on the concentration of malondialdehyde and 7-ketocholesterol and the Activity of superoxide dismutase in blood Plasma of rabbits with experimental Hypercholesterolemia, Research report. Fluoride 2008;41(3):199–205.

    CAS  Google Scholar 

  21. Inkielewicz I, Rogowska M, Krechmiak J, Gdansk. Lipid peroxidation and antioxidant enzyme activity in rats exposed to fluoride and ethanol, Research report. Fluoride 2006;39(1):53–59.

    CAS  Google Scholar 

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Correspondence to Sunil Kumar Gupta.

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Ailani, V., Gupta, R.C., Gupta, S.K. et al. Oxidative stress in cases of chronic fluoride intoxication. Indian J Clin Biochem 24, 426 (2009). https://doi.org/10.1007/s12291-009-0076-0

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  • DOI: https://doi.org/10.1007/s12291-009-0076-0

Key Words

  • Fluorosis
  • Super oxide dismutase
  • Serum fluoride
  • Urinary fluoride
  • Drinking water fluoride