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Cellular immune response to infection by different genotypes of hepatitis C virus

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Abstract

The present study aimed to estimate the cellular immune response to infection by different HCV genotypes in some Egyptian patients with chronic liver disease. HCV-RNA and HCV genotypes were conducted using type-specific primers to amplify the core region of HCV genome. The levels of IFN-gamma and TNF-alpha, PIIINP, SOD enzyme were also determined. The results showed that genotype 4 was the prevalent one (88.57%).Genotype 3 was found mixed with genotype 2 and 4 (11.42% and 34.28% respectively). Genotype 1 was absent from patients under study. No significant differences were observed in the level of IFN-gamma interferon, SOD enzyme and ALT enzyme between those genotypes. On the other hand the level of TNF-alpha was greatly increased in patients infected with mixed HCV genotypes 3, 4 compared with patients with genotype 4 and those infected with mixed genotypes 2, 3. Otherwise PIIINP was decreased significantly of the same group compared with other groups.

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Authors and Affiliations

  1. Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Sadat City, Egypt

    Mohamed El-Shahat Ebeid

  2. Zoology Department, Faculty of Science (Damietta), Mansoura University, Damietta, Egypt

    Kadry Abdel-Kader El-Bakry

  3. Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Minufiya University, Sadat City, Egypt, P.O 22857-79

    Mohamed El-Shahat Ebeid

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  1. Mohamed El-Shahat Ebeid
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  2. Kadry Abdel-Kader El-Bakry
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Correspondence to Mohamed El-Shahat Ebeid.

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Ebeid, M.ES., El-Bakry, K.AK. Cellular immune response to infection by different genotypes of hepatitis C virus. Indian J Clin Biochem 24, 234–240 (2009). https://doi.org/10.1007/s12291-009-0044-8

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  • DOI: https://doi.org/10.1007/s12291-009-0044-8

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