Abstract
Aspergillus fumigatus a fungal pathogen is implicated in a spectrum of allergic and invasive disorders in humans. Validation of transcriptome of pathogen is essential for understanding its virulence mechanism and to identify new therapeutic targets/diagnostic markers. In order to rapidly identify genes of Aspergillus fumigatus we adopted sequencing of cDNA clones. Our earlier effort has lead to identification of 68 expressed sequence tags of Aspergillus fumigatus. Present study describes 52 more expressed sequence tags generated by sequencing 200 phage clones of a non-normalized cDNA library. One of the cDNA clones comprised of the complete coding region for tetratricopeptide repeat domain protein gene. Various homology search algorithms were employed to assign functions to expressed sequence tags coding for hypothetical proteins, and relevance of these expressed sequence tags or their protein products as drug targets/diagnostic markers was examined by searching for homologues in fungi and human.
Similar content being viewed by others
References
Nierman WC, Pain A, Anderson MJ, Wortman JR, Kim HS, Arroyo J, et al. Genomic sequence of the pathogenic and allergenic filamentous fungus Aspergillus fumigatus. Nature 2005; 438:1151–1156.
Ronning CM, Fedorova ND, Bowyer P, Coulson R, Goldman G, Kim HS, et al. Genomics of Aspergillus fumigatus. Rev Iberoam Micol 2005; 22:223–228.
Rudd S. Expressed sequence tags: alternative or complement to whole genome sequences? Trends Plant Sci 2003; 8:321–329.
Brendel V, Zhu W. Computational modeling of gene structure in Arabidopsis thaliana. Plant Mol Biol 2002; 48: 49–58.
Haas BJ, Volfovsky N, Town CD, Troukhan M, Alexandrov N, Feldmann KA, Flavell RB, White O, Salzberg SL. Fulllength messenger RNA sequences greatly improve genome annotation. Genome Biol 2002; 3:RESEARCH0029.
Shankar J, Nigam S, Saxena S, Madan T, Sarma PU. Identification and assignment of function to the genes of Aspergillus fumigatus expressed at 37 degrees C. J Eukaryot Microbiol 2004; 51:428–432.
D’Andrea LD, Regan L. TPR proteins: the versatile helix. Trends Biochem Sci 2003; 28:655–662.
O’Donnell KL, Osmani AH, Osmani SA, Morris NR. bimA encodes a member of the tetratricopeptide repeat family of proteins and is required for the completion of mitosis in Aspergillus nidulans. J Cell Sci 1991; 99:711–719.
Feng B, Zhao CH, Tanaka S, Imanaka H, Imamura K, Nakanishi K. TPR domain of Ser/Thr phosphatase of Aspergillus oryzae shows no auto-inhibitory effect on the dephosphorylation activity. Int J Biol Macromol 2007; 41: 281–285.
Chung S, Mondon P, Chang YC, Kwon-Chung KJ. Cryptococcus neoformans with a mutation in the tetratricopeptide repeat-containing gene, CCN1, causes subcutaneous lesions but fails to cause systemic infection. Infect Immun 2003; 71:1988–1994.
Kaneko A, Umeyama T, Utena-Abe Y, Yamagoe S, Niimi M, Uehara Y. Tcc1p, a novel protein containing the tetratricopeptide repeat motif, interacts with Tup1p to regulate morphological transition and virulence in Candida albicans. Eukaryot Cell 2006; 5:1894–1905.
Kessler MM, Willins DA, Zeng Q, Del Mastro RG, Cook R, Doucette-Stamm L, et al. The use of direct cDNA selection to rapidly and effectively identify genes in the fungus Aspergillus fumigatus. Fungal Genet Biol 2002; 36:59–70.
Markaryan A, Zaborina O, Punj V, Chakrabarty AM. Adenylate kinase as a virulence factor of Pseudomonas aeruginosa. J Bacteriol 2001; 183:3345–3352.
Solano C, Garcia B, Valle J, Berasain C, Ghigo JM, Gamazo C, Lasa I. Genetic analysis of Salmonella enteritidis biofilm formation: critical role of cellulose. Mol Microbiol 2002; 43:793–808.
Clemmer KM, Sturgill GM, Veenstra A, Rather PN. Functional characterization of Escherichia coli GlpG and additional rhomboid proteins using an aarA mutant of Providencia stuartii. J Bacteriol 2006; 188:3415–3419.
Shen HD, Lin WL, Tam MF, Chou H, Wang CW, Tsai JJ, Wang SR, Han SH. Identification of vacuolar serine proteinase as a major allergen of Aspergillus fumigatus by immunoblotting and N-terminal amino acid sequence analysis. Clin Experimental Allergy 2001; 31:295–302.
Purkayastha S, Madan T, Shah A, Krishnamurthy HG, Sarma PU. Multifunctional antigens of A. fumigatus and specific antibodies. Appl Biochem Biotechnol 2000; 83:271–283; discussion 283–276, 297–313.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Upadhyay, S.K., Shankar, J., Singh, Y. et al. Expressed sequence tags of Aspergillus fumigatus: Extension of catalogue and their evaluation as putative drug targets and/or diagnostic markers. Indian J Clin Biochem 24, 131–136 (2009). https://doi.org/10.1007/s12291-009-0024-z
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12291-009-0024-z