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Indian Journal of Clinical Biochemistry

, Volume 23, Issue 3, pp 227–232 | Cite as

Normal delivery induced stress alters glutathione peroxidase and TNF-α in elderly primigravidas mononuclear cells

  • Nasreen Noor
  • Najmul IslamEmail author
  • Shagufta Moin
  • Abbas Ali Mahdi
  • Sapna Jaiswal
  • Farzana Bano
Article

Abstract

To probe the glutathione peroxidase (GPx) activity coupled to ROS and TNF-α levels in younger versus elderly primigravidas. Monocytes were isolated from blood of young and elderly primigravidas after 12 hrs, 24 hrs and 14 days postpartum. Age-matched healthy female donors served as controls. Thereafter, the above monocytes were subjected to evaluation of GPx activity, levels of pro-inflammatory cytokine TNF-α and reactivity/specificity towards anti-TNF-α antibody. Suppression in GPx activity with simultaneous enhancement of TNF-α levels in monocytes was observed after 12 hrs and 24 hrs of spontaneous labor pain-induced maternal delivery through normal vaginal route. Conversely, an enhancement of GPx activity and decrease in TNF-α levels were observed on day 14 of postpartum, thereby indicating reversal of the impaired neutralizing mechanisms. The antioxidant network combating ROS was stronger in younger primigravidas. Capability of TNF-α and ROS suppression was somewhat lesser in elderly primigravidas even 14 days of postpartum via normal vaginal route. The capability of GPx activity enhancement with simultaneous suppression in ROS and TNF-α, decreases in elderly primigravidas. This may act as one of the important parameters leading to a variety of complications encountered by elderly primigravidas.

Key words

Tumor Necrosis Factor-alpha (TNF-α) Monocytes Glutathione peroxidase 

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Copyright information

© Association of Clinical Biochemists of India 2008

Authors and Affiliations

  • Nasreen Noor
    • 1
  • Najmul Islam
    • 1
    • 2
    Email author
  • Shagufta Moin
    • 1
  • Abbas Ali Mahdi
    • 1
    • 3
  • Sapna Jaiswal
    • 1
    • 3
  • Farzana Bano
    • 1
    • 4
  1. 1.Department of Obstetrics & GynecologyJ. N. Medical College, A. M. U.AligarhIndia
  2. 2.Department of Biochemistry, Faculty of MedicineJ. N. Medical College, A. M. U.AligarhIndia
  3. 3.Department of BiochemistryKing George’s Medical UniversityLucknowIndia
  4. 4.Department of BiochemistryEra’s Lucknow Medical CollegeLucknowIndia

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