Abstract
Hemolysis, a crucial feature of Sickle cell disease (SCD), is a key player for cellular activation leading to various complications including thrombosis. In response to hemolysis, platelets get activated and release components that are necessary for further platelet activation and aggregation. Thus, it is believed that platelets contribute to the development of thrombotic complications. Platelets in SCD are expected to be affected due to common cause of hemolysis. To measure the surface markers of platelets including P-Selectin, Phosphatidyl Serine and integrin αIIbβ3 in SCD patients and healthy controls in order to understand the status of the platelets in SCD. To measure the surface markers of activated platelets using flow cytometry. Since mitochondria and calcium play an important role in cellular functions, the mitochondrial membrane potential and calcium content of platelets in SCD were also evaluated using flow cytometry. In the present study, we have observed significant increase of calcium level in SCD platelets. Further, the loss of mitochondrial membrane potential in SCD platelets was found to be significantly higher when compared to platelets of healthy controls. Though the surface markers of activated platelets in SCD remain unchanged, increased level of calcium and mitochondrial membrane potential loss suggest that the platelets in SCD are more prone to become activated. In order to understand the status of the platelets in SCD, apart from the surface markers, it is also important to assess the calcium levels and mitochondrial membrane potential of platelets.
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Acknowledgements
The authors thank the funding agency, Science and Engineering Research Board, Department of Science and Technology (DST-SERB), Government of India, INDIA (Grant ID: EMR/2016/000955) for supporting this study. The authors also thank the participants who volunteered for this study.
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SM, CLR and KK collected samples, performed experiments and collected data. RR helped with the sample collection and clinical data collection. FA critically revised the manuscript for important intellectual content. RS and MM supported the clinical data, interpreted and validated the manuscript. MK designed the study, supervised the project, interpreted the data and wrote the manuscript. All authors have approved the final article.
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This study was approved by the institutional human ethics committee (IEC-514). All procedures followed were in accordance with the ethical standards of the institutional committee on human experimentation and with the Helsinki Declaration of 1975, as revised in 2008 (5). All persons gave their informed consent prior to their inclusion in the study. Informed consent was obtained from all patients for being included in the study.
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Maharana, S., Roy, C.L., Kishor, K. et al. Depolarized Mitochondrial Membrane Potential and Elevated Calcium in Platelets of Sickle Cell Disease. Indian J Hematol Blood Transfus 39, 565–571 (2023). https://doi.org/10.1007/s12288-023-01640-7
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DOI: https://doi.org/10.1007/s12288-023-01640-7