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Evaluation of a New Method of Leukocyte Extractions from the Leukoreduction Filter

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Abstract

This study’s purpose was to optimize the leukocyte extraction protocol and evaluate the efficacy of this new protocol. 12BioR blood filters were collected from Tehran Blood Transfusion Center. A twosyringe system and Multi-step rinsing were designed for cell extraction. The final purpose of this optimization was: (1) removed the residual RBCs, (2) reversed the leukocyte trapping process, and (3) remove the microparticles to obtain the high yield of target cells. Finally, Extracted cells were evaluated by Automated Cell count; Samples smear differential cell count, Trypan blue, and Annexin-PI staining. The results showed that on average 11.88 × 108 ± 3.32 leukocytes recovered after indirect washing and that the mean count of granulocytes, lymphocytes, and Monocyte in this sample was 5.24 ± 2.18 × 108, 5.57 ± 1.74 × 108, and 0.56 ± 0.38 × 108 respectively. Also, the mean percent of manual differential cell count after concentration was 42.81%, 41.80%, and 15.82% for granulocytes, lymphocytes, and monocytes respectively. Moreover, viability and apoptosis assay showed > 95% viability in mononuclear cells recovered from LRFs. It is concluded that the use of a double-syringe system and RBC and microparticles removal from leukoreduction filters lead to acceptable viable leukocyte count that can be used in in vitro and in vivo studies.

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Acknowledgments

The study was financially and materially supported by the High Institute for Research and Education in Transfusion Medicine.

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Author notes

  1. Co-first authors: Zahra Abbasi Malati, Ali Akbar Pourfathollah.

Authors and Affiliations

  1. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

    Zahra Abbasi Malati, Ali Akbar Pourfathollah, Sahar Balagholi & Mohammad Reza Javan

  2. Immunology Department Faculty Medical Science, Tarbiat Modares University, Tehran, Iran

    Ali Akbar Pourfathollah

  3. Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Rasul Dabbaghi & Sahar Balagholi

Authors
  1. Zahra Abbasi Malati
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  2. Ali Akbar Pourfathollah
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  3. Rasul Dabbaghi
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  4. Sahar Balagholi
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  5. Mohammad Reza Javan
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Correspondence to Sahar Balagholi.

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Malati, Z.A., Pourfathollah, A.A., Dabbaghi, R. et al. Evaluation of a New Method of Leukocyte Extractions from the Leukoreduction Filter. Indian J Hematol Blood Transfus 39, 478–486 (2023). https://doi.org/10.1007/s12288-022-01618-x

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  • DOI: https://doi.org/10.1007/s12288-022-01618-x

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