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Down-Regulation of CXCR4 in Mesenchymal Stem Cells by Septic Serum

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Indian Journal of Hematology and Blood Transfusion Aims and scope Submit manuscript

Abstract

Background

Sepsis is one of the main concerns of health and one of the leading causes of death in hospitals. It is essential to manage sepsis in hospitalized patients. In recent years, cell therapy has been considered as a new approach to treat sepsis. This study evaluated the effect of CXCR4 as one of the main proteins involved in the homing of mesenchymal stem cells in the sepsis serum in mice model.

Methods

Mouse sepsis model was induced by injection of E.coli and biochemical analyses was done to confirm the organ failure. Mesenchymal stem cells (MSCs) derived from bone marrow were separated into sepsis and control groups. In the sepsis serum group, MSCs were treated with sepsis serum at two time points: 24 and 48 h. Quantitative RT-PCR and flow cytometry were performed to determine the mRNA expression of CXCR4 in sepsis serum group compared to control group. Also, a migration assay was done to assess the migration capacity of bone marrow MSCs during inflammation and treatment in sepsis.

Results

Our result showed that treatment with sepsis serum can control migration by decrease in CXCR4 level (P ≤ 0.05) compared to control group. Moreover it was also reported that sepsis serum decreased mRNA expression of CXCR4 in MScs.

Conclusions

In our study, MSCs treated with septic serum were no longer able to migrate . Probably many variables such as source, dose, injection time, and injection route of MSCs after sepsis induction in the animal models are key factors for successful cell therapy.

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Acknowledgements

The present work was supported financially by Shahroud University of Medical Sciences ( Grant No.9828).

Funding

The present work was supported financially by Shahroud University of Medical Sciences (Grant No.9828).

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Authors and Affiliations

  1. School of Biology, Damghan University, Damghan, Iran

    Mohammad Amin Ghanbari & Taghi Lashkar Bolouki

  2. School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Pirasteh Norouzi

  3. Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Fatemeh Sadat Bitaraf

  4. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Haniye Bakhshi

  5. Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran

    Amir Atashi

Authors
  1. Mohammad Amin Ghanbari
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  2. Taghi Lashkar Bolouki
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  3. Pirasteh Norouzi
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  4. Fatemeh Sadat Bitaraf
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  5. Haniye Bakhshi
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  6. Amir Atashi
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Correspondence to Amir Atashi.

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Ghanbari, M.A., Lashkar Bolouki, T., Norouzi, P. et al. Down-Regulation of CXCR4 in Mesenchymal Stem Cells by Septic Serum. Indian J Hematol Blood Transfus 38, 718–725 (2022). https://doi.org/10.1007/s12288-022-01560-y

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  • DOI: https://doi.org/10.1007/s12288-022-01560-y

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