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Frequency of HLA alleles and KIR Ligands in Acute Myeloid Leukemia in Indian Cohort

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Abstract

Relationship between various combinations of KIR ligands and HLA alleles have been studied in several diseases. The aim of this retrospective study was to estimate the frequency of HLA alleles and KIR ligands among acute myeloid leukemia patients and healthy controls in order to examine the possible association of HLA alleles and KIR ligands with AML. A total of 439 acute myeloid leukemia patients and 1317 unrelated, healthy ethnic Indian controls were included in the study. HLA typing was performed using PCR-SSP. KIR ligands were assigned by using the KIR ligand Calculator. The frequency of HLA alleles and KIR ligands in patients was then compared with the controls. As compared to controls, frequencies of HLA-A*03 and HLA-B*35 were increased in AML patients, whereas, that of HLA-C*03 was decreased. Frequencies of HLA-A*03 and HLA-C*15 were increased in male patients, however, no significant difference was observed in female patients as compared to controls. In the pediatric group, the frequencies of HLA-A*01 was decreased and that of HLA-A*03 and HLA-B*18 were increased, whereas, frequencies of HLA-B*13 was decreased and that of HLA-B*27 was increased in the adult patients. In the haplotype analysis, the frequency of HLA-A*24/B*35/DRB1*15 was increased in overall patients. In adult group, the frequency of HLA-A*01/B*44/DRB1*07 was increased in patients than in controls. No significant association was observed between KIR ligands and susceptibility/ protection to AML. Our results indicate that certain HLA alleles and haplotypes have presumptive positive or negative role in conferring protection/susceptibility to AML.

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Abbreviations

AML:

Acute myeloid Leukemia

KIR:

Killer cell immunoglobulin-like receptor

NK cell:

Natural killer cell

LD:

Linkage disequilibrium

HSCT:

Hematopoietic stem cell transplantation

ALL:

Acute lymphoblastic Leukemia

WAS:

Genome-wide association studies

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Acknowledgements

We wish to acknowledge the technical assistance by Ms. Pallavi Rane, Department of Statistics, Advanced Centre for Treatment, Research and Education in Cancer.

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Authors and Affiliations

  1. Scientific Officer “E”, Transplant Immunology and Immunogenetics Laboratory, Advanced Centre for Treatment, Research and Education in Cancer, CCE, Tata Memorial Centre, Plot No.1 & 2, Sector 22, Kharghar, Navi Mumbai, 410210, India

    Selma Zenia D’Silva, Shalaka Kadam, Manisha Tambe & Meenakshi Singh

  2. Department of Transfusion Medicine, Tata Memorial Hospital, Mumbai, India

    Anand Bodade

  3. Department of Oncology, St. Jude Children’s Research Hospital, Memphis, TN, 38105, USA

    Sandeep Dhanda

  4. Department of Medical Oncology, Tata Memorial Hospital, Mumbai, India

    Bhausaheb Bagal, Manju Sengar, Hasmukh Jain, Lingaraj Nayak & Avinash Bonda

  5. Homi Bhabha National Institute (HBNI), Mumbai, India

    Bhausaheb Bagal, Manju Sengar, Hasmukh Jain, Lingaraj Nayak, Avinash Bonda, Sachin Punatar, Anant Gokarn, Navin Khattry & Meenakshi Singh

  6. Advanced Centre for Treatment, Research and Education in Cancer, Bone Marrow Transplant Unit, Tata Memorial Centre, Kharghar, India

    Sachin Punatar, Anant Gokarn & Navin Khattry

Authors
  1. Selma Zenia D’Silva
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  2. Anand Bodade
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  3. Shalaka Kadam
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  4. Manisha Tambe
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  10. Avinash Bonda
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  11. Sachin Punatar
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  12. Anant Gokarn
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  13. Navin Khattry
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  14. Meenakshi Singh
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Correspondence to Meenakshi Singh.

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D’Silva, S.Z., Bodade, A., Kadam, S. et al. Frequency of HLA alleles and KIR Ligands in Acute Myeloid Leukemia in Indian Cohort. Indian J Hematol Blood Transfus 39, 50–56 (2023). https://doi.org/10.1007/s12288-022-01550-0

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