Role of Therapeutic Plasma Exchange in Resolution of Hyperhemolysis in a Patient Living with HIV/AIDS (PLHA) Diagnosed with Paroxysmal Cold Haemoglobinuria: A Case Report”


Paroxysmal cold haemoglobinuria (PCH) is an acquired cause of haemolytic anaemia. It is caused by the biphasic IgG antibodies that sensitize and attach the red cells at lower temperature; detach and rebind on rewarming. Donath-Landsteiner antibody test is the diagnostic test for PCH. Management of PCH mainly includes supportive care but sometimes disease becomes severe and life-threatening. We describe a similar severe and life-threatening case of PCH who was managed by plasma exchange followed by packed red cell transfusion.


Paroxysmal cold haemoglobinuria (PCH) is a rare type of autoimmune haemolytic anemia (AIHA) seen due to IgG biphasic hemolysin which is capable of binding the red cells at lower temperature resulting in complement mediated intravascular haemolysis at body temperature. The hemolysin involved was first described by Donath-Landsteiner in 1904 and is known as donath-landsteiner (DL) antibody. It is described as an abrupt onset of severe anaemia and hemoglobinuria.[1]

Historically, PCH was commonly encountered in patients with secondary or tertiary syphilis, which is rarely seen now. [1, 2] It presents commonly as an acute transient condition that is secondary to a viral infection (mumps, measles, chicken pox, Epstein-Barr virus, cytomegalovirus, influenza, parvovirus B19, coxsackie virus and adenovirus) particularly in young children. However, it may also be associated with immunologic disorders such as diffuse large B-cell lymphoma and chronic lymphocytic leukemia. At times the cause may remain unknown i.e. a specific pathogen is not identified in these patients. [3,4,5,6,7]

Pathophysiologically, the disease represents molecular mimicry and cross reactivity of the antibodies stimulated by the viruses with the normal red cells, resulting in alteration of T suppressor cell function leading to autoantibody formation [1]. The DL antibody (autoantibody) has shown specificity for the P antigen which is expressed on red cells and microorganism cell wall. Donath-landsteiner (DL) test is considered as the gold standard test for diagnosing patients presenting with PCH. [2] There are handful of case reports demonstrating the role of plasma exchange in PCH. Burman AR et al. [8] in 2002 tried first plasma exchange on paediatric patient and observed clinical improvement. Here, we report a case highlighting the role of therapeutic plasma exchange (TPE) in resolving hyperhaemolysis in a 35-year-old female patient living with HIV/AIDS (PLHA) diagnosed with PCH.

Case Report

A 35 year old female presented with complaints of palpitations, generalized weakness, shortness of breath and dizziness of one month duration which was associated with dark colour stools and urine since three days. She was admitted to our hospital with above mentioned complaints for further management. Patient was a known case of HIV/AIDS (diagnosed in 2006) in clinical stage 3 on anti-retroviral therapy (ART: zidovudine, lamivudine and nevirapine). Past history also revealed that patient was a chronic alcoholic and has also been diagnosed with chronic pancreatitis. On examination, patient had severe pallor, sinus tachycardia with pulse rate of 112 beats per minute, breathlessness with SpO2 of 94% on room air and blood pressure of 100/60 mm Hg. She received supportive care along with fluid resuscitation, intravenous (I.V.) iron, vitamin B12, folic acid, pantoprazole, and ceftriaxone. At the time of admission, her baseline haemoglobin was 3.7 g/dL for which she also transfused with two unit of packed red blood cells (PRBCs). Table 1 summarises her base line laboratory parameters.

Table 1 Base line laboratory parameters

Next day, clinicians noticed that patient was passing dark coloured urine. Laboratory investigations revealed further fall in haemoglobin, raised total bilirubin, increased lactate dehydrogenase (LDH) levels, undetectable haptoglobin levels and haemoglobinuria. Peripheral smear revealed anisopoikilocytosis, spherocytes and polychromasia which were suggestive of haemolytic anemia and bone marrow aspirate showed normoblast with megaloblast with myeloid series in various stages of maturation and normal megakaryocytes. Her ART was changed to abacavir, lamivudine and efavirenz to rule out drug induced haemolysis. Samples were sent for transfusion reaction workup as per the transfusion medicine (TM) departmental policy.

In view of intravascular haemolysis with haemoglobinuria, the initial differential diagnoses [9] for this patient included (1) mismatched blood transfusion: which was ruled out with repeat blood grouping and cross match confirmation, (2) septicaemia: which was ruled out as patient’s blood cultures were negative, (3) microangiopathic anaemia, march haemoglobinuria, favism: which were ruled out after taking detailed history with absence of symptoms, (4) paroxysmal nocturnal haemoglobinuria (PNH): flowcytometry was normal for CD-55 and CD-59, (5) G6PD deficiency: which was ruled out as G6PD levels were within normal limits and (6) paroxysmal cold haemoglobinuria (PCH): patient’s mono-specific direct antiglobulin test (DAT) was negative for IgG and 1 + positive for C3d. Direct and indirect DL test was positive, though the P specificity of antibody could not be confirmed. ELISA was positive for parvovirus B19 IgG antibodies but polymerase chain reaction (PCR) was negative for parvovirus DNA.

In view of non-responding anaemia and risk of flare up of opportunistic infections in HIV/AIDS with immunosuppressive therapy, the case was discussed with TM specialist and therapeutic plasma exchange (TPE) followed by PRBC transfusion was planned. A total of three procedures of TPE were performed using central venous access with 4% albumin as primary replacement fluid and ACD-A as anticoagulant. An average of one plasma volume was removed per procedure. TPE procedure 1 was initiated at patient hemoglobin (Hb) of 3.2 g/dl and haematocrit of 10%. Total 3731 ml of whole blood was processed and 2511 ml of the plasma was removed (one plasma volume) and replaced with 2458 ml of 4% albumin, normal saline followed by two-unit PRBC transfusion. Removed plasma was dark brown as shown in Fig. 1. Hemoglobin content of the removed plasma bag was calculated as 5 g (0.2 g/dl). On next day patient Hb was raised to 8 g/dl but patient persistently complained of dark colour urine though she was symptomatically better with improvement in palpitation, breathlessness and increase in appetite. Monospecific DCT was negative after the procedure. Hb was maintained above 8 g/dl for next three days but fell to 6 g/dl on the fourth day. Second procedure of TPE was planned and about 3488 ml of whole blood was processed and 1787 ml of the plasma was removed and replaced with 1653 ml of 4% albumin, normal saline followed by one-unit PRBC transfusion. Hb content of removed plasma bag was 1.2 g i.e. lesser than compared with procedure 1. Patient improved symptomatically and did not complain any further haemoglobinuria following the second procedure of TPE. Before discharge, third procedure of plasma exchange was done and removed plasma was clear straw-coloured. No complications noted during and after the procedure. Average plasma volume exchanged was around 2500 ml and replaced with 2300 ml with albumin and saline. Patient also received 10% calcium gluconate prophylactically during the procedure to rule out any citrate related toxicity. In view of low haemoglobin at the beginning of the procedure, cell separator was primed with PRBC. Mid-procedure patient’s sample was drawn to check any signs of haemolysis. Table 2 summarises her plasma exchange procedural details.

Fig. 1

a, b and c Colour of removed plasma bag during the 1st, 2nd and 3rd plasma exchange session. d, e and f Urine samples demonstrating improvement with respect to clearance of PFHb and reduction in bilirubin levels from 1st to the 3rd session of plasma exchange

Table 2 Details of plasma-exchange procedure

At the time of discharge (day 15 of admission), patient was symptomatically better and hemodynamically stable. She was discharged on pantaprozole, folic acid, oral iron supplements and ART therapy. At two months follow up, patient was well and laboratory investigations showed haemoglobin level of 10.5 g/dL and negative DAT.


PCH was first described by Dressler in 1854 but DL test which is considered gold standard for PCH was first described by Donath Landsteiner in 1904. [10] Antibody identified in PCH is biphasic IgG which was responsible for paroxysms of severe anaemia and haemoglobinuria. [1] Red cells get sensitized at lower temperature where antibody binds and latter detaches and rebinds at body temperature activating the complement and causes intravascular red cell lysis. This antibody has been called “biphasic” due to different temperature of attachment and causing lysis of red cells. [6] In the past PCH was seen in patients of secondary and tertiary syphilis. Other causes include viral illnesses such as mumps, measles, chicken pox, Epstein-Barr virus, cytomegalovirus, influenza, parvovirus B19, coxsackie virus and adenovirus, and immunologic disorders such as diffuse large B-cell lymphoma and chronic lymphocytic leukemia. [3,4,5,6,7] In this present case we discuss the role of therapeutic plasma exchange (TPE) in resolving hyperhemolysis in a 35-year-old female patient living with HIV/AIDS (PLHA) diagnosed with PCH.

Despite repeated compatible PRBC transfusions, haemoglobin of the patient was not raised and after every transfusion patient complaint of haemoglobinuria. Transfusion of warmed PRBC is not advocated as causative antibody does not react in compatibility testing at temperature greater than 4 °C [11]. This temperature dependent feature of PCH differentiates it from cold agglutinin disease (CAD) in which cold autoantibodies have a titre of > 1000 and are reactive at 30 °C. [11] American society for Apheresis (ASFA) mentions regarding role of plasma exchange in autoimmune hemolytic anemia- severe cold and warm type, however there is no specific mention regarding paroxysmal cold haemoglobinuria. Rational for plasma exchange in PCH was to remove low affinity DL antibody which are mostly intravascular. Because of transient and brief production of DL antibody and without significant rebound increase in production, plasma exchange was tried to remove any formed DL antibodies. Similar case was reported by Burman AR et al. [8] in 2002 in which plasma exchange was first tried and authors observed similar improvement as we observed in our case. After first procedure of plasma exchange patient’s general condition improved. Removed plasma was dark brown in colour with haemoglobin content of 5 g. With the third procedure of plasma exchange, removed plasma was straw coloured. There was continuous improvement in general condition and laboratory parameters thereafter.


This is probably the first reported case on paroxysmal cold haemoglobinuria who underwent plasma exchange in HIV positive patient presenting with intravascular haemolysis. Patient had dramatic response after the procedure. Plasma exchange can be added as an effective adjuvant therapy in treating severe presentation of the disease especially in patients where immunosuppression is not advocated.



Packed red blood cell


Direct antiglobulin test


Lactate dehydrogenase


People living with HIV/AIDS


Therapeutic plasma exchange

DL antibody:

Donath-landsteiner antibody


Autoimmune haemolytic anaemia


Anti retroviral therapy


Paroxysmal nocturnal haemoglobinuria


Paroxysmal cold haemoglobinuria


American society of apheresis


Transfusion medicine


Indirect antiglobulin test


Acid citrate dextrose-A


Human immunodeficiency disease


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We are thankful to the patient for permitting us to use their case for presentation.



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Correspondence to Anubhav Gupta.

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Gupta, A., Chaudhary, K., Bhatta, A. et al. Role of Therapeutic Plasma Exchange in Resolution of Hyperhemolysis in a Patient Living with HIV/AIDS (PLHA) Diagnosed with Paroxysmal Cold Haemoglobinuria: A Case Report”. Indian J Hematol Blood Transfus (2020).

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  • Paroxysmal cold haemoglobinuria
  • Plasma exchange
  • Donath-landsteiner (DL) antibody