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A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma

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Thawed fresh frozen plasma (FFP) if not used within 6 h, may have to be discarded due to the risk of contamination and uncertainty about its quality. The main objective of this study was to evaluate the levels of coagulation Factor II (FII), Factor VIII (FVIII), fibrinogen and bacterial growth in thawed refrozen FFP. Thirty FFP samples were collected from healthy donors. FFP were thawed in water bath at 37 °C for 20–25 min. Approximately 10 mL of plasma from each FFP unit was tested for FII, FVIII, fibrinogen and sterility. The thawed FFP units were then kept at 4 °C for 6 h before being refrozen and stored at − 20 °C. Two weeks later, the refrozen FFP were thawed again and representative samples were analysed as before. There was a significant decline in the mean FVIII level, from 155.77% to 85.6% at second thaw. The mean FII level increased significantly from 74.9% to 82%, whereas the mean fibrinogen level fell from 3.34g/L to 3.28 g/L, but the decline was not statistically significant. There was no bacterial contamination in all samples at both time points. Refrozen plasma may be considered as an alternative to the storage of thawed unused FFP provided they are kept in a controlled environment to reduce wastage. These thawed refrozen FFP can be used later in bleeding cases like other FFP as the levels of FVIII are still within the standard haematology range (0.5–2 IU/mL) and above the minimal level of 30% coagulation factors required for adequate haemostasis.

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We would like to thank UKM Medical Centre for providing a research Grant (Code: FF-2016-075). We would also like to thank the staff, UKMMC for their technical help in this study.


This study was funded by UKM Medical Centre (Research Grant Code: FF-2016 075).

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Correspondence to Chooi Fun Leong.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee (Secretariat for Medical Research and Innovation, National Medical Research Registry) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.”

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Informed consent was obtained from all individual participants included in the study. A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma.

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Draman, R., Yousuf, R., Abdul Aziz, S. et al. A Preliminary Study on Coagulation Parameters and Sterility of Thawed Refrozen Fresh Frozen Plasma. Indian J Hematol Blood Transfus 36, 112–116 (2020).

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